Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence

Invasive candidiasis is caused mainly by Candida albicans, but other Candida species have increasing etiologies. These species show different virulence and susceptibility levels to antifungal drugs. The aims of this study were to evaluate the usefulness of the non-conventional model Caenorhabditis e...

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Autores: Ortega Riveros, Marcelo, De la Pinta Aresti, Iker, Marcos Arias, Cristina, Ezpeleta Lobato, Guillermo, Quindós Andrés, Guillermo, Eraso Barrio, María Elena
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/76045
Acceso en línea:http://hdl.handle.net/10810/76045
Access Level:acceso abierto
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spelling Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulenceOrtega Riveros, MarceloDe la Pinta Aresti, IkerMarcos Arias, CristinaEzpeleta Lobato, GuillermoQuindós Andrés, GuillermoEraso Barrio, María ElenaInvasive candidiasis is caused mainly by Candida albicans, but other Candida species have increasing etiologies. These species show different virulence and susceptibility levels to antifungal drugs. The aims of this study were to evaluate the usefulness of the non-conventional model Caenorhabditis elegans to assess the in vivo virulence of seven different Candida species and to compare the virulence in vivo with the in vitro production of proteinases and phospholipases, hemolytic activity and biofilm development capacity. One culture collection strain of each of seven Candida species (C. albicans, Candida dubliniensis, Candida glabrata, Candida krusei, Candida metapsilosis, Candida orthopsilosis and Candida parapsilosis) was studied. A double mutant C. elegans AU37 strain (glp-4;sek-1) was infected with Candida by ingestion, and the analysis of nematode survival was performed in liquid medium every 24 h until 120 h. Candida establishes a persistent lethal infection in the C. elegans intestinal tract. C. albicans and C. krusei were the most pathogenic species, whereas C. dubliniensis infection showed the lowest mortality. C. albicans was the only species with phospholipase activity, was the greatest producer of aspartyl proteinase and had a higher hemolytic activity. C. albicans and C. krusei caused higher mortality than the rest of the Candida species studied in the C. elegans model of candidiasis.Springer202520252017info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/76045reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://doi.org/10.1007/s11046-017-0142-8info:eu-repo/semantics/openAccess© 2017, Springer Science Business Media Dordrechtoai:addi.ehu.eus:10810/760452026-06-18T09:23:17Z
dc.title.none.fl_str_mv Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence
title Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence
spellingShingle Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence
Ortega Riveros, Marcelo
title_short Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence
title_full Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence
title_fullStr Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence
title_full_unstemmed Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence
title_sort Usefulness of the non-conventional Caenorhabditis elegans model to assess Candida virulence
dc.creator.none.fl_str_mv Ortega Riveros, Marcelo
De la Pinta Aresti, Iker
Marcos Arias, Cristina
Ezpeleta Lobato, Guillermo
Quindós Andrés, Guillermo
Eraso Barrio, María Elena
author Ortega Riveros, Marcelo
author_facet Ortega Riveros, Marcelo
De la Pinta Aresti, Iker
Marcos Arias, Cristina
Ezpeleta Lobato, Guillermo
Quindós Andrés, Guillermo
Eraso Barrio, María Elena
author_role author
author2 De la Pinta Aresti, Iker
Marcos Arias, Cristina
Ezpeleta Lobato, Guillermo
Quindós Andrés, Guillermo
Eraso Barrio, María Elena
author2_role author
author
author
author
author
description Invasive candidiasis is caused mainly by Candida albicans, but other Candida species have increasing etiologies. These species show different virulence and susceptibility levels to antifungal drugs. The aims of this study were to evaluate the usefulness of the non-conventional model Caenorhabditis elegans to assess the in vivo virulence of seven different Candida species and to compare the virulence in vivo with the in vitro production of proteinases and phospholipases, hemolytic activity and biofilm development capacity. One culture collection strain of each of seven Candida species (C. albicans, Candida dubliniensis, Candida glabrata, Candida krusei, Candida metapsilosis, Candida orthopsilosis and Candida parapsilosis) was studied. A double mutant C. elegans AU37 strain (glp-4;sek-1) was infected with Candida by ingestion, and the analysis of nematode survival was performed in liquid medium every 24 h until 120 h. Candida establishes a persistent lethal infection in the C. elegans intestinal tract. C. albicans and C. krusei were the most pathogenic species, whereas C. dubliniensis infection showed the lowest mortality. C. albicans was the only species with phospholipase activity, was the greatest producer of aspartyl proteinase and had a higher hemolytic activity. C. albicans and C. krusei caused higher mortality than the rest of the Candida species studied in the C. elegans model of candidiasis.
publishDate 2017
dc.date.none.fl_str_mv 2017
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/76045
url http://hdl.handle.net/10810/76045
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1007/s11046-017-0142-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
© 2017, Springer Science Business Media Dordrecht
eu_rights_str_mv openAccess
rights_invalid_str_mv © 2017, Springer Science Business Media Dordrecht
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
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