Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals

Metals play an essential role in cellular homeostasis and are key components of several formulations currently used in the clinic. Synchrotron-based X-ray microscopy at submicron resolution is a powerful approach to map intracellular elemental distributions and to monitor how these patterns change u...

Full description

Bibliographic Details
Authors: Tamargo Azpilicueta, Joaquín, Giner Arroyo, Rafael Luis, Rivero-García, Pablo, Díaz-Moreno, Sofía, Gómez González, Miguel A, Telfer, Abbey, Campanella, Michelangelo, Rosa Acosta, Miguel Ángel de la, Díaz Moreno, Irene
Format: article
Status:Published version
Publication Date:2026
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:dnet:idus________::2e5cab247861029a7cbffe548f234fb6
Online Access:https://hdl.handle.net/11441/186062
https://doi.org/10.1002/cmtd.202500152
Access Level:Open access
Keyword:Metallomics
Metalloproteins
Spectromicroscopy
X-ray absorption spectroscopy
X-ray fluorescence microscopy
id ES_090e11dbb3cc21ec55fbfa8683120fa6
oai_identifier_str oai:dnet:idus________::2e5cab247861029a7cbffe548f234fb6
network_acronym_str ES
network_name_str España
repository_id_str
spelling Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular MetalsTamargo Azpilicueta, JoaquínGiner Arroyo, Rafael LuisRivero-García, PabloDíaz-Moreno, SofíaGómez González, Miguel ATelfer, AbbeyCampanella, MichelangeloRosa Acosta, Miguel Ángel de laDíaz Moreno, IreneMetallomicsMetalloproteinsSpectromicroscopyX-ray absorption spectroscopyX-ray fluorescence microscopyMetals play an essential role in cellular homeostasis and are key components of several formulations currently used in the clinic. Synchrotron-based X-ray microscopy at submicron resolution is a powerful approach to map intracellular elemental distributions and to monitor how these patterns change upon genetic or pharmacological perturbations. However, existing sample-preparation protocols often rely on costly and highly specialized equipment for vitrification and dehydration, limiting their widespread adoption. Here, we present an adapted plunge-freezing and freeze-drying workflow that enables the preparation of mammalian cell samples for X-ray fluorescence (XRF) and X-ray absorption spectroscopy (XAS) studies with submicron resolution in a cost-effective and versatile manner. Furthermore, we define acquisition parameters optimized for the reliable detection of low-abundance metals, such as endogenous iron. We anticipate that this accessible protocol will facilitate the broader implementation of synchrotron-based inner-shell spectromicroscopy in cell biology.WileyBioquímica Vegetal y Biología MolecularMinisterio de Ciencia, Innovación y Universidades (MICIU). EspañaAgencia Estatal de Investigación. EspañaJunta de Andalucía2026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/186062https://doi.org/10.1002/cmtd.202500152reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésChemistry - Methods, 6 (3), e202500152. PID2021-126663NB-I00PID2024-157414NB-I00BIO-198info:eu-repo/semantics/openAccessoai:dnet:idus________::2e5cab247861029a7cbffe548f234fb62026-06-17T12:51:07Z
dc.title.none.fl_str_mv Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals
title Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals
spellingShingle Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals
Tamargo Azpilicueta, Joaquín
Metallomics
Metalloproteins
Spectromicroscopy
X-ray absorption spectroscopy
X-ray fluorescence microscopy
title_short Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals
title_full Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals
title_fullStr Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals
title_full_unstemmed Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals
title_sort Broadening the Workflow for Synchrotron-Based X-Ray Fluorescence and X-Ray Absorption Spectroscopy Imaging of Low-Abundance Cellular Metals
dc.creator.none.fl_str_mv Tamargo Azpilicueta, Joaquín
Giner Arroyo, Rafael Luis
Rivero-García, Pablo
Díaz-Moreno, Sofía
Gómez González, Miguel A
Telfer, Abbey
Campanella, Michelangelo
Rosa Acosta, Miguel Ángel de la
Díaz Moreno, Irene
author Tamargo Azpilicueta, Joaquín
author_facet Tamargo Azpilicueta, Joaquín
Giner Arroyo, Rafael Luis
Rivero-García, Pablo
Díaz-Moreno, Sofía
Gómez González, Miguel A
Telfer, Abbey
Campanella, Michelangelo
Rosa Acosta, Miguel Ángel de la
Díaz Moreno, Irene
author_role author
author2 Giner Arroyo, Rafael Luis
Rivero-García, Pablo
Díaz-Moreno, Sofía
Gómez González, Miguel A
Telfer, Abbey
Campanella, Michelangelo
Rosa Acosta, Miguel Ángel de la
Díaz Moreno, Irene
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Ministerio de Ciencia, Innovación y Universidades (MICIU). España
Agencia Estatal de Investigación. España
Junta de Andalucía
dc.subject.none.fl_str_mv Metallomics
Metalloproteins
Spectromicroscopy
X-ray absorption spectroscopy
X-ray fluorescence microscopy
topic Metallomics
Metalloproteins
Spectromicroscopy
X-ray absorption spectroscopy
X-ray fluorescence microscopy
description Metals play an essential role in cellular homeostasis and are key components of several formulations currently used in the clinic. Synchrotron-based X-ray microscopy at submicron resolution is a powerful approach to map intracellular elemental distributions and to monitor how these patterns change upon genetic or pharmacological perturbations. However, existing sample-preparation protocols often rely on costly and highly specialized equipment for vitrification and dehydration, limiting their widespread adoption. Here, we present an adapted plunge-freezing and freeze-drying workflow that enables the preparation of mammalian cell samples for X-ray fluorescence (XRF) and X-ray absorption spectroscopy (XAS) studies with submicron resolution in a cost-effective and versatile manner. Furthermore, we define acquisition parameters optimized for the reliable detection of low-abundance metals, such as endogenous iron. We anticipate that this accessible protocol will facilitate the broader implementation of synchrotron-based inner-shell spectromicroscopy in cell biology.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/186062
https://doi.org/10.1002/cmtd.202500152
url https://hdl.handle.net/11441/186062
https://doi.org/10.1002/cmtd.202500152
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Chemistry - Methods, 6 (3), e202500152.
PID2021-126663NB-I00
PID2024-157414NB-I00
BIO-198
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403083741593600
score 15,198674

Similar Items