Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples

Blue mold caused by Penicillium expansum is a major postharvest disease of pome fruit. Several mechanisms possibly involved in P. expansum pathogenicity and virulence. However, factors that mediate pathogenicity and virulence are largely not yet characterized. In this work we analyzed P. expansum pr...

Descripción completa

Detalles Bibliográficos
Autores: Levin, E., Kishore, A., Ballester Frutos, Ana Rosa, Raphael, G., Feigenberg, O., Liu, Y., Norelli, J., González-Candelas, Luis, Wisniewski, Michael, Droby, Samir
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/173615
Acceso en línea:http://hdl.handle.net/10261/173615
Access Level:acceso abierto
Palabra clave:Penicillium expansum
Virulence
Host-pathogen interaction
Secretome
Authophagy
id ES_0909f5fd9da67dbad321c11989e2e3e4
oai_identifier_str oai:digital.csic.es:10261/173615
network_acronym_str ES
network_name_str España
repository_id_str
spelling Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on applesLevin, E.Kishore, A.Ballester Frutos, Ana RosaRaphael, G.Feigenberg, O.Liu, Y.Norelli, J.González-Candelas, LuisWisniewski, MichaelDroby, SamirPenicillium expansumVirulenceHost-pathogen interactionSecretomeAuthophagyBlue mold caused by Penicillium expansum is a major postharvest disease of pome fruit. Several mechanisms possibly involved in P. expansum pathogenicity and virulence. However, factors that mediate pathogenicity and virulence are largely not yet characterized. In this work we analyzed P. expansum predicted secretome to reveal potential genes that have a role in host-pathogen interaction. A prediction pipeline was designed using an approach that combines common effector features, transcriptomic data and homology to proteins reported to be involved in pathogenicity of other pytopathogenic fungi. Among 297 genes predicted in P. expansum secretome, 103 genes (35%) were found to code for hydrolytic enzymes. The majority of the secreted enzymes are carbohydrate-degrading enzymes among which five coding for pectin-degrading enzymes are highly induced during the infection and decay of apple fruit by P. expansum, indicating that they may represent an important aspect of pathogenicity and virulence. Applying the pipeline we have predicted 17 candidate genes coding for proteins that are most likely involved in pathogenicity and virulence. One of the top candidates is a subtilisin-related peptidase, S8 (PePRT), proteolytic enzyme highly expressed in planta, and potentially involved in authophagy process. Deletion of PePRT-coding gene resulted in reduced virulence of P. expansum on apples. Moreover, ΔPeprt exhibited decreased sporulation as well as affected mycelial morphology and internal mycelial cell structure.We thank Israel Science Foundation (ISF) and National Natural Science Foundation of China (NSFC): 1936/14, awarded to Prof. Samir Droby and Prof. Yongsheng Liu. Israel Binational Agricultural Research and Development Fund (BARD), US-4774-14C: awarded to Prof. Samir Droby and Dr. Michael Wisniewski, and Spanish Ministry of Economy and Competitiveness (Research Grant AGL2014-55802-R) and the Generalitat Valenciana, Spain (Grant PROMETEOII/2014/027) awarded to Dr. González-Candelas.Peer reviewedElsevierGeneralitat ValencianaIsrael Science FoundationNational Natural Science Foundation of ChinaUnited States - Israel Binational Agricultural Research and Development FundMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/173615reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-55802-Rhttps://doi.org/10.1016/j.postharvbio.2018.10.011Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1736152026-05-22T06:33:51Z
dc.title.none.fl_str_mv Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
title Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
spellingShingle Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
Levin, E.
Penicillium expansum
Virulence
Host-pathogen interaction
Secretome
Authophagy
title_short Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
title_full Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
title_fullStr Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
title_full_unstemmed Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
title_sort Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
dc.creator.none.fl_str_mv Levin, E.
Kishore, A.
Ballester Frutos, Ana Rosa
Raphael, G.
Feigenberg, O.
Liu, Y.
Norelli, J.
González-Candelas, Luis
Wisniewski, Michael
Droby, Samir
author Levin, E.
author_facet Levin, E.
Kishore, A.
Ballester Frutos, Ana Rosa
Raphael, G.
Feigenberg, O.
Liu, Y.
Norelli, J.
González-Candelas, Luis
Wisniewski, Michael
Droby, Samir
author_role author
author2 Kishore, A.
Ballester Frutos, Ana Rosa
Raphael, G.
Feigenberg, O.
Liu, Y.
Norelli, J.
González-Candelas, Luis
Wisniewski, Michael
Droby, Samir
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Generalitat Valenciana
Israel Science Foundation
National Natural Science Foundation of China
United States - Israel Binational Agricultural Research and Development Fund
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Penicillium expansum
Virulence
Host-pathogen interaction
Secretome
Authophagy
topic Penicillium expansum
Virulence
Host-pathogen interaction
Secretome
Authophagy
description Blue mold caused by Penicillium expansum is a major postharvest disease of pome fruit. Several mechanisms possibly involved in P. expansum pathogenicity and virulence. However, factors that mediate pathogenicity and virulence are largely not yet characterized. In this work we analyzed P. expansum predicted secretome to reveal potential genes that have a role in host-pathogen interaction. A prediction pipeline was designed using an approach that combines common effector features, transcriptomic data and homology to proteins reported to be involved in pathogenicity of other pytopathogenic fungi. Among 297 genes predicted in P. expansum secretome, 103 genes (35%) were found to code for hydrolytic enzymes. The majority of the secreted enzymes are carbohydrate-degrading enzymes among which five coding for pectin-degrading enzymes are highly induced during the infection and decay of apple fruit by P. expansum, indicating that they may represent an important aspect of pathogenicity and virulence. Applying the pipeline we have predicted 17 candidate genes coding for proteins that are most likely involved in pathogenicity and virulence. One of the top candidates is a subtilisin-related peptidase, S8 (PePRT), proteolytic enzyme highly expressed in planta, and potentially involved in authophagy process. Deletion of PePRT-coding gene resulted in reduced virulence of P. expansum on apples. Moreover, ΔPeprt exhibited decreased sporulation as well as affected mycelial morphology and internal mycelial cell structure.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/173615
url http://hdl.handle.net/10261/173615
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-55802-R
https://doi.org/10.1016/j.postharvbio.2018.10.011
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403082940481536
score 15.812429

Similares en LA Referencia