Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)

Trypanosoma cruzi, the causative agent of Chagas disease, is divided into six Discrete Typing Units (DTUs): TcI-TcVI. We aimed to identify T. cruzi DTUs in Latin-American migrants in the Barcelona area (Spain) and to assess different molecular typing approaches for the characterization of T. cruzi g...

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Autores: Abras Feliu, Alba, Gállego Culleré, M. (Montserrat), Muñoz Batet, M. Carmen, Juiz, Natalia, Ramírez, Juan Carlos, Cura, Carolina, Tebar, Silvia, Fernández Arévalo, Anna, Pinazo, Maria-Jesus, Torre, Leonardo de la, Posada, Elizabeth, Navarro, Ferran, Espinal, Paula, Ballart Ferrer, J. Cristina, Portús Vinyeta, Montserrat, Gascón i Brustenga, Joaquim, Schijman, Alejandro G.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/204082
Acceso en línea:https://hdl.handle.net/2445/204082
Access Level:acceso abierto
Palabra clave:Malaltia de Chagas
Tripanosoma
Chagas' disease
Trypanosoma
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spelling Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)Abras Feliu, AlbaGállego Culleré, M. (Montserrat)Muñoz Batet, M. CarmenJuiz, NataliaRamírez, Juan CarlosCura, CarolinaTebar, SilviaFernández Arévalo, AnnaPinazo, Maria-JesusTorre, Leonardo de laPosada, ElizabethNavarro, FerranEspinal, PaulaBallart Ferrer, J. CristinaPortús Vinyeta, MontserratGascón i Brustenga, JoaquimSchijman, Alejandro G.Malaltia de ChagasTripanosomaChagas' diseaseTrypanosomaTrypanosoma cruzi, the causative agent of Chagas disease, is divided into six Discrete Typing Units (DTUs): TcI-TcVI. We aimed to identify T. cruzi DTUs in Latin-American migrants in the Barcelona area (Spain) and to assess different molecular typing approaches for the characterization of T. cruzi genotypes. Seventy-five peripheral blood samples were analyzed by two real-time PCR methods (qPCR) based on satellite DNA (SatDNA) and kinetoplastid DNA (kDNA). The 20 samples testing positive in both methods, all belonging to Bolivian individuals, were submitted to DTU characterization using two PCR-based flowcharts: multiplex qPCR using TaqMan probes (MTq-PCR), and conventional PCR. These samples were also studied by sequencing the SatDNA and classified as type I (TcI/III), type II (TcII/IV) and type I/II hybrid (TcV/VI). Ten out of the 20 samples gave positive results in the flowcharts: TcV (5 samples), TcII/V/VI (3) and mixed infections by TcV plus TcII (1) and TcV plus TcII/VI (1). By SatDNA sequencing, we classified the 20 samples, 19 as type I/II and one as type I. The most frequent DTU identified by both flowcharts, and suggested by SatDNA sequencing in the remaining samples with low parasitic loads, TcV, is common in Bolivia and predominant in peripheral blood. The mixed infection by TcV-TcII was detected for the first time simultaneously in Bolivian migrants. PCR-based flowcharts are very useful to characterize DTUs during acute infection. SatDNA sequence analysis cannot discriminate T. cruzi populations at the level of a single DTU but it enabled us to increase the number of characterized cases in chronically infected patients.Elsevier2023202320172023info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion6 p.application/pdfhttps://hdl.handle.net/2445/204082Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.parint.2016.12.003Parasitology International, 2017, vol. 66, num.2, p. 83-88https://doi.org/10.1016/j.parint.2016.12.003cc-by-nc-nd (c) Japanese Society of Parasitology, 2017http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2040822026-05-29T05:05:01Z
dc.title.none.fl_str_mv Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
title Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
spellingShingle Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
Abras Feliu, Alba
Malaltia de Chagas
Tripanosoma
Chagas' disease
Trypanosoma
title_short Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
title_full Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
title_fullStr Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
title_full_unstemmed Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
title_sort Identification of Trypanosoma cruzi Discrete Typing Units (DTUs) in Latin-American migrants in Barcelona (Spain)
dc.creator.none.fl_str_mv Abras Feliu, Alba
Gállego Culleré, M. (Montserrat)
Muñoz Batet, M. Carmen
Juiz, Natalia
Ramírez, Juan Carlos
Cura, Carolina
Tebar, Silvia
Fernández Arévalo, Anna
Pinazo, Maria-Jesus
Torre, Leonardo de la
Posada, Elizabeth
Navarro, Ferran
Espinal, Paula
Ballart Ferrer, J. Cristina
Portús Vinyeta, Montserrat
Gascón i Brustenga, Joaquim
Schijman, Alejandro G.
author Abras Feliu, Alba
author_facet Abras Feliu, Alba
Gállego Culleré, M. (Montserrat)
Muñoz Batet, M. Carmen
Juiz, Natalia
Ramírez, Juan Carlos
Cura, Carolina
Tebar, Silvia
Fernández Arévalo, Anna
Pinazo, Maria-Jesus
Torre, Leonardo de la
Posada, Elizabeth
Navarro, Ferran
Espinal, Paula
Ballart Ferrer, J. Cristina
Portús Vinyeta, Montserrat
Gascón i Brustenga, Joaquim
Schijman, Alejandro G.
author_role author
author2 Gállego Culleré, M. (Montserrat)
Muñoz Batet, M. Carmen
Juiz, Natalia
Ramírez, Juan Carlos
Cura, Carolina
Tebar, Silvia
Fernández Arévalo, Anna
Pinazo, Maria-Jesus
Torre, Leonardo de la
Posada, Elizabeth
Navarro, Ferran
Espinal, Paula
Ballart Ferrer, J. Cristina
Portús Vinyeta, Montserrat
Gascón i Brustenga, Joaquim
Schijman, Alejandro G.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malaltia de Chagas
Tripanosoma
Chagas' disease
Trypanosoma
topic Malaltia de Chagas
Tripanosoma
Chagas' disease
Trypanosoma
description Trypanosoma cruzi, the causative agent of Chagas disease, is divided into six Discrete Typing Units (DTUs): TcI-TcVI. We aimed to identify T. cruzi DTUs in Latin-American migrants in the Barcelona area (Spain) and to assess different molecular typing approaches for the characterization of T. cruzi genotypes. Seventy-five peripheral blood samples were analyzed by two real-time PCR methods (qPCR) based on satellite DNA (SatDNA) and kinetoplastid DNA (kDNA). The 20 samples testing positive in both methods, all belonging to Bolivian individuals, were submitted to DTU characterization using two PCR-based flowcharts: multiplex qPCR using TaqMan probes (MTq-PCR), and conventional PCR. These samples were also studied by sequencing the SatDNA and classified as type I (TcI/III), type II (TcII/IV) and type I/II hybrid (TcV/VI). Ten out of the 20 samples gave positive results in the flowcharts: TcV (5 samples), TcII/V/VI (3) and mixed infections by TcV plus TcII (1) and TcV plus TcII/VI (1). By SatDNA sequencing, we classified the 20 samples, 19 as type I/II and one as type I. The most frequent DTU identified by both flowcharts, and suggested by SatDNA sequencing in the remaining samples with low parasitic loads, TcV, is common in Bolivia and predominant in peripheral blood. The mixed infection by TcV-TcII was detected for the first time simultaneously in Bolivian migrants. PCR-based flowcharts are very useful to characterize DTUs during acute infection. SatDNA sequence analysis cannot discriminate T. cruzi populations at the level of a single DTU but it enabled us to increase the number of characterized cases in chronically infected patients.
publishDate 2017
dc.date.none.fl_str_mv 2017
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/204082
url https://hdl.handle.net/2445/204082
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.parint.2016.12.003
Parasitology International, 2017, vol. 66, num.2, p. 83-88
https://doi.org/10.1016/j.parint.2016.12.003
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Japanese Society of Parasitology, 2017
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Japanese Society of Parasitology, 2017
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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