Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria

Electroporation is the most widely used and efficient method to transform mycobacteria. Through this technique, fast- and slow-growing mycobacteria with smooth and rough morphotypes have been successfully transformed. However, transformation efficiencies differ widely between species and strains. In...

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Detalhes bibliográficos
Autores: Campo Pérez, Víctor, Cendra Gascón, María del Mar|||0000-0002-2100-2406, Julian Gómez, Esther, Torrents Serra, Eduard
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/359689
Acesso em linha:https://hdl.handle.net/2117/359689
https://dx.doi.org/10.1016/j.nbt.2021.02.003
Access Level:acceso abierto
Palavra-chave:Electroporation
Mycobacteria
Clump
Desalted DNA
Mycolicibacterium brumae
Mycobacteroides abscessus
Electroporació
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
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spelling Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteriaCampo Pérez, VíctorCendra Gascón, María del Mar|||0000-0002-2100-2406Julian Gómez, EstherTorrents Serra, EduardElectroporationMycobacteriaClumpDesalted DNAElectroporationMycolicibacterium brumaeMycobacteroides abscessusElectroporacióÀrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::MicrobiologiaElectroporation is the most widely used and efficient method to transform mycobacteria. Through this technique, fast- and slow-growing mycobacteria with smooth and rough morphotypes have been successfully transformed. However, transformation efficiencies differ widely between species and strains. In this study, the smooth and rough morphotypes of Mycobacteroides abscessus and Mycolicibacterium brumae were used to improve current electroporation procedures for fast-growing rough mycobacteriaElsevier20212021-07-0120222022-01-14journal articlehttp://purl.org/coar/resource_type/c_6501CVoRhttp://purl.org/coar/version/c_e19f295774971610info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/359689https://dx.doi.org/10.1016/j.nbt.2021.02.003reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3596892026-05-27T15:37:01Z
dc.title.none.fl_str_mv Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
title Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
spellingShingle Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
Campo Pérez, Víctor
Electroporation
Mycobacteria
Clump
Desalted DNA
Electroporation
Mycolicibacterium brumae
Mycobacteroides abscessus
Electroporació
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
title_short Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
title_full Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
title_fullStr Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
title_full_unstemmed Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
title_sort Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria
dc.creator.none.fl_str_mv Campo Pérez, Víctor
Cendra Gascón, María del Mar|||0000-0002-2100-2406
Julian Gómez, Esther
Torrents Serra, Eduard
author Campo Pérez, Víctor
author_facet Campo Pérez, Víctor
Cendra Gascón, María del Mar|||0000-0002-2100-2406
Julian Gómez, Esther
Torrents Serra, Eduard
author_role author
author2 Cendra Gascón, María del Mar|||0000-0002-2100-2406
Julian Gómez, Esther
Torrents Serra, Eduard
author2_role author
author
author
dc.subject.none.fl_str_mv Electroporation
Mycobacteria
Clump
Desalted DNA
Electroporation
Mycolicibacterium brumae
Mycobacteroides abscessus
Electroporació
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
topic Electroporation
Mycobacteria
Clump
Desalted DNA
Electroporation
Mycolicibacterium brumae
Mycobacteroides abscessus
Electroporació
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia
description Electroporation is the most widely used and efficient method to transform mycobacteria. Through this technique, fast- and slow-growing mycobacteria with smooth and rough morphotypes have been successfully transformed. However, transformation efficiencies differ widely between species and strains. In this study, the smooth and rough morphotypes of Mycobacteroides abscessus and Mycolicibacterium brumae were used to improve current electroporation procedures for fast-growing rough mycobacteria
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-07-01
2022
2022-01-14
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
CVoR
http://purl.org/coar/version/c_e19f295774971610
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/359689
https://dx.doi.org/10.1016/j.nbt.2021.02.003
url https://hdl.handle.net/2117/359689
https://dx.doi.org/10.1016/j.nbt.2021.02.003
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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