Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold

Adult articular cartilage has a limited capacity for growth and regeneration and, with injury, new cellular or biomaterial-based therapeutic platforms are required to promote repair. Tissue engineering aims to produce cartilage-like tissues that recreate the complex mechanical and biological propert...

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Detalles Bibliográficos
Autores: Semino, Carlos, Abella, Jordi, Recha Sancho, Lourdes Georgina, Moutos, Franklin T., Guilak, Farshid
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/1002
Acceso en línea:http://hdl.handle.net/20.500.14342/1002
https://doi.org/10.3390/ma9060472
Access Level:acceso abierto
Palabra clave:Biomimètica
Cartílags
Cartilage tissue engineering
3D cell culture
Human chondrocytes
Cell differentiation
Biomimetic materials
57
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spelling Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffoldSemino, CarlosAbella, JordiRecha Sancho, Lourdes GeorginaMoutos, Franklin T.Guilak, FarshidBiomimèticaCartílagsCartilage tissue engineering3D cell cultureHuman chondrocytesCell differentiationBiomimetic materials57Adult articular cartilage has a limited capacity for growth and regeneration and, with injury, new cellular or biomaterial-based therapeutic platforms are required to promote repair. Tissue engineering aims to produce cartilage-like tissues that recreate the complex mechanical and biological properties found in vivo. In this study, a unique composite scaffold was developed by infiltrating a three-dimensional (3D) woven microfiber poly (ε-caprolactone) (PCL) scaffold with the RAD16-I self-assembling nanofibers to obtain multi-scale functional and biomimetic tissue-engineered constructs. The scaffold was seeded with expanded dedifferentiated human articular chondrocytes and cultured for four weeks in control and chondrogenic growth conditions. The composite constructs were compared to control constructs obtained by culturing cells with 3D woven PCL scaffolds or RAD16-I independently. High viability and homogeneous cell distribution were observed in all three scaffolds used during the term of the culture. Moreover, gene and protein expression profiles revealed that chondrogenic markers were favored in the presence of RAD16-I peptide (PCL/RAD composite or alone) under chondrogenic induction conditions. Further, constructs displayed positive staining for toluidine blue, indicating the presence of synthesized proteoglycans. Finally, mechanical testing showed that constructs containing the PCL scaffold maintained the initial shape and viscoelastic behavior throughout the culture period, while constructs with RAD16-I scaffold alone contracted during culture time into a stiffer and compacted structure. Altogether, these results suggest that this new composite scaffold provides important mechanical requirements for a cartilage replacement, while providing a biomimetic microenvironment to re-establish the chondrogenic phenotype of human expanded articular chondrocytes.MDPIUniversitat Ramon Llull. IQS2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion18 p.http://hdl.handle.net/20.500.14342/1002https://doi.org/10.3390/ma9060472reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésMaterials. Vol.9, n.6 (2016), 472info:eu-repo/grantAgreement/AO Foundation/CRP ACI/BIOCARTinfo:eu-repo/grantAgreement/AO Foundation/CRP ACI/OSTEOCHON3Dinfo:eu-repo/grantAgreement/NHI/AR50245/info:eu-repo/grantAgreement/NHI/AR48852/info:eu-repo/grantAgreement/NHI/AG15768/info:eu-repo/grantAgreement/NHI/AR48182/info:eu-repo/grantAgreement/NHI/AG46927/info:eu-repo/grantAgreement/SUR del DEC i FSE/FI/2015FI_B2 00109Attribution 4.0 International© L'autor/ahttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/10022026-05-29T05:05:01Z
dc.title.none.fl_str_mv Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold
title Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold
spellingShingle Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold
Semino, Carlos
Biomimètica
Cartílags
Cartilage tissue engineering
3D cell culture
Human chondrocytes
Cell differentiation
Biomimetic materials
57
title_short Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold
title_full Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold
title_fullStr Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold
title_full_unstemmed Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold
title_sort Dedifferentiated human articular chondrocytes redifferentiate to a cartilage-like tissue phenotype in a poly(ε-caprolactone)/self-assembling peptide composite scaffold
dc.creator.none.fl_str_mv Semino, Carlos
Abella, Jordi
Recha Sancho, Lourdes Georgina
Moutos, Franklin T.
Guilak, Farshid
author Semino, Carlos
author_facet Semino, Carlos
Abella, Jordi
Recha Sancho, Lourdes Georgina
Moutos, Franklin T.
Guilak, Farshid
author_role author
author2 Abella, Jordi
Recha Sancho, Lourdes Georgina
Moutos, Franklin T.
Guilak, Farshid
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Biomimètica
Cartílags
Cartilage tissue engineering
3D cell culture
Human chondrocytes
Cell differentiation
Biomimetic materials
57
topic Biomimètica
Cartílags
Cartilage tissue engineering
3D cell culture
Human chondrocytes
Cell differentiation
Biomimetic materials
57
description Adult articular cartilage has a limited capacity for growth and regeneration and, with injury, new cellular or biomaterial-based therapeutic platforms are required to promote repair. Tissue engineering aims to produce cartilage-like tissues that recreate the complex mechanical and biological properties found in vivo. In this study, a unique composite scaffold was developed by infiltrating a three-dimensional (3D) woven microfiber poly (ε-caprolactone) (PCL) scaffold with the RAD16-I self-assembling nanofibers to obtain multi-scale functional and biomimetic tissue-engineered constructs. The scaffold was seeded with expanded dedifferentiated human articular chondrocytes and cultured for four weeks in control and chondrogenic growth conditions. The composite constructs were compared to control constructs obtained by culturing cells with 3D woven PCL scaffolds or RAD16-I independently. High viability and homogeneous cell distribution were observed in all three scaffolds used during the term of the culture. Moreover, gene and protein expression profiles revealed that chondrogenic markers were favored in the presence of RAD16-I peptide (PCL/RAD composite or alone) under chondrogenic induction conditions. Further, constructs displayed positive staining for toluidine blue, indicating the presence of synthesized proteoglycans. Finally, mechanical testing showed that constructs containing the PCL scaffold maintained the initial shape and viscoelastic behavior throughout the culture period, while constructs with RAD16-I scaffold alone contracted during culture time into a stiffer and compacted structure. Altogether, these results suggest that this new composite scaffold provides important mechanical requirements for a cartilage replacement, while providing a biomimetic microenvironment to re-establish the chondrogenic phenotype of human expanded articular chondrocytes.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/1002
https://doi.org/10.3390/ma9060472
url http://hdl.handle.net/20.500.14342/1002
https://doi.org/10.3390/ma9060472
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Materials. Vol.9, n.6 (2016), 472
info:eu-repo/grantAgreement/AO Foundation/CRP ACI/BIOCART
info:eu-repo/grantAgreement/AO Foundation/CRP ACI/OSTEOCHON3D
info:eu-repo/grantAgreement/NHI/AR50245/
info:eu-repo/grantAgreement/NHI/AR48852/
info:eu-repo/grantAgreement/NHI/AG15768/
info:eu-repo/grantAgreement/NHI/AR48182/
info:eu-repo/grantAgreement/NHI/AG46927/
info:eu-repo/grantAgreement/SUR del DEC i FSE/FI/2015FI_B2 00109
dc.rights.none.fl_str_mv Attribution 4.0 International
© L'autor/a
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
© L'autor/a
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 18 p.
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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