Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay

Fenhexamid is a new-generation agrochemical extensively used nowadays to protect high valuable crops and fruits from fungus attacks. In this paper, the development of an indirect competitive immunoassay for the determination of fenhexamid residues in fruit samples is described. After optimisation of...

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Detalles Bibliográficos
Autores: Esteve Turrillas, Francesc A., Abad Fuentes, Antonio, Mercader Badia, Josep Vicent
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/112287
Acceso en línea:http://hdl.handle.net/10261/112287
Access Level:acceso abierto
Palabra clave:Monoclonal antibody
Rapid methods
Pesticide residues
Fungicide
Food safety
Validation
Hapten
ELISA
Immunoassay
Fenhexamid
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spelling Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assayEsteve Turrillas, Francesc A.Abad Fuentes, AntonioMercader Badia, Josep VicentMonoclonal antibodyRapid methodsPesticide residuesFungicideFood safetyValidationHaptenELISAImmunoassayFenhexamidFenhexamid is a new-generation agrochemical extensively used nowadays to protect high valuable crops and fruits from fungus attacks. In this paper, the development of an indirect competitive immunoassay for the determination of fenhexamid residues in fruit samples is described. After optimisation of immunoreagent concentrations, the assay, based on monoclonal antibody FHo4#27, showed high sensitivity, and selectivity, with a limit of detection for fenhexamid standards in buffer of 0.04μg/L. The influence of several physico-chemical factors, including pH, ionic strength, solvent tolerance, and matrix interferences on the analytical parameters of the standard curve was studied. For spiked liquid food samples like grape must, just a direct 1:50 dilution in water was sufficient to attain recoveries between 100% and 110%. For kiwifruit and strawberry samples, a double extraction procedure with methanol is proposed in this study, which afforded recovery values that ranged between 103% and 116%. The limit of quantification of the developed assay was 5μg/kg for grape must, 10μg/kg for kiwifruits, and 25μg/kg for strawberries. The optimised assay was compared with a reference procedure based on liquid chromatography using incurred samples from the market, and an excellent correlation between both methods was found. © 2010 Elsevier Ltd.This work was financed by Generalitat Valenciana (GVPRE/2008/236) and Ministerio de Educación y Ciencia (AGL2006-12750-C02-01/ALI) and cofinanced by FEDER funds. J.V. Mercader and F.A. Esteve-Turrillas were hired by the CSIC; the former under a contract of the I3P Program and the latter under the Juan de la Cierva Program of the Spanish Ministry of Innovation and Science and cofinanced by the European Social Fund.Peer ReviewedElsevier2015201520112015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/112287reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1122872026-05-22T06:33:51Z
dc.title.none.fl_str_mv Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay
title Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay
spellingShingle Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay
Esteve Turrillas, Francesc A.
Monoclonal antibody
Rapid methods
Pesticide residues
Fungicide
Food safety
Validation
Hapten
ELISA
Immunoassay
Fenhexamid
title_short Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay
title_full Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay
title_fullStr Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay
title_full_unstemmed Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay
title_sort Determination of fenhexamid residues in grape must, kiwifruit, and strawberry samples by enzyme-linked immunosorbent assay
dc.creator.none.fl_str_mv Esteve Turrillas, Francesc A.
Abad Fuentes, Antonio
Mercader Badia, Josep Vicent
author Esteve Turrillas, Francesc A.
author_facet Esteve Turrillas, Francesc A.
Abad Fuentes, Antonio
Mercader Badia, Josep Vicent
author_role author
author2 Abad Fuentes, Antonio
Mercader Badia, Josep Vicent
author2_role author
author
dc.subject.none.fl_str_mv Monoclonal antibody
Rapid methods
Pesticide residues
Fungicide
Food safety
Validation
Hapten
ELISA
Immunoassay
Fenhexamid
topic Monoclonal antibody
Rapid methods
Pesticide residues
Fungicide
Food safety
Validation
Hapten
ELISA
Immunoassay
Fenhexamid
description Fenhexamid is a new-generation agrochemical extensively used nowadays to protect high valuable crops and fruits from fungus attacks. In this paper, the development of an indirect competitive immunoassay for the determination of fenhexamid residues in fruit samples is described. After optimisation of immunoreagent concentrations, the assay, based on monoclonal antibody FHo4#27, showed high sensitivity, and selectivity, with a limit of detection for fenhexamid standards in buffer of 0.04μg/L. The influence of several physico-chemical factors, including pH, ionic strength, solvent tolerance, and matrix interferences on the analytical parameters of the standard curve was studied. For spiked liquid food samples like grape must, just a direct 1:50 dilution in water was sufficient to attain recoveries between 100% and 110%. For kiwifruit and strawberry samples, a double extraction procedure with methanol is proposed in this study, which afforded recovery values that ranged between 103% and 116%. The limit of quantification of the developed assay was 5μg/kg for grape must, 10μg/kg for kiwifruits, and 25μg/kg for strawberries. The optimised assay was compared with a reference procedure based on liquid chromatography using incurred samples from the market, and an excellent correlation between both methods was found. © 2010 Elsevier Ltd.
publishDate 2011
dc.date.none.fl_str_mv 2011
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/112287
url http://hdl.handle.net/10261/112287
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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