Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 14-04-2021

Detalles Bibliográficos
Autor: Kidibule, Peter Elias
Tipo de recurso: tesis doctoral
Fecha de publicación:2021
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/696376
Acceso en línea:http://hdl.handle.net/10486/696376
Access Level:acceso abierto
Palabra clave:Oligosacáridos
Enzimas
Biopolímeros
Biología y Biomedicina / Biología
id ES_08c7152b9d3ebe3ca5a18e3e928e341d
oai_identifier_str oai:repositorio.uam.es:10486/696376
network_acronym_str ES
network_name_str España
repository_id_str
spelling Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalystsUtilización de materiales quitinolíticos para la obtención de oligosacáridos bioactivos. Caracterización de biocatalizadores y productosKidibule, Peter EliasOligosacáridosEnzimasBiopolímerosBiología y Biomedicina / BiologíaTesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 14-04-2021Esta tesis tiene embargado el acceso al texto completo hasta el 14-10-2022Chitin, the most abundant natural biopolymer in aquatic environment, is composed of repeating β-1-4 linked N-acetyl-β-D-glucosamine (GlcNAc) units, and constitutes the structural component of protective biological structures such as fungal cell walls, crustaceans and insect exoskeletons. Recently, enzymes acting on chitin and chitosan (a deacetylation product of chitin) have gained strong interest due to wide range of biotechnological applications of these polymer derivatives. The biological activity of chitin, chitosan and/ particularly chitooligosaccharides (COS) has made these compounds the targets for the industrial and medical sectors. In this context chitinases and chitosanases are essential glycosyl hydrolases (GH) for the biotransformation of chitin and chitosan into COS by hydrolyzing the β-1,4-linkages. In this work, two fungal chitinases, the endo-chitinase Chit33 and the exo-chitinase Chit42, both from Trichoderma harzianum were expressed in Pichia pastoris, using fed-batch fermentation, to 0.63 and 3 g/L, respectively. The heterologous proteins were purified, characterized biochemically and their kinetic parameters on different types of chitinolytic materials analyzed. Numerous variants of both enzymes were also obtained using rational targeted mutation based on their structural determinants. Utilization of the two enzymes (Chit42 also immobilized on magnetic nanoparticles and chitosan beads) as well as that of commercial preparations based on Bacillus spp cells extracts were analyzed for their application in the production of fully acetylated (faCOS), fully deacetylated (fdCOS) and partially acetylated (paCOS) COS mixtures. The product profiles were analyzed, when possible characterized using different chromatographic techniques and MALDI-TOF mass spectrometry, and their biological activity evaluated. COS mixtures in the range of 0.5-2 and 2-10 kDa produced from colloidal chitin and chitosan clearly exhibited antioxidant activity. The mixtures of fdCOS and faCOS apparently showed anti-inflammatory activity and some of them even a neuroprotective effect. The biological properties of COS obtained from chitin and chitosan gives biotechnological potential to both, the biocatalyst producing them and the products that they generateThis work was supported by the EU EMFF-Blue Economy-2018-FISH4FISH-863697 project, the Spanish Ministry of Economy and Competitiveness [BIO2016-76601-C3-1/-2], the Spanish Ministry of Science and Innovation PID2019-105838RB-C32/-31, Fundación Ramón Areces [XIX Call of Research Grants in Life and Material Sciences], and by an institutional grant from Fundación Ramón Areces to the Centro de Biología MolecularFernández Lobato, MaríaDepartamento de Biología MolecularFacultad de Ciencias20212021-04-14doctoral thesishttp://purl.org/coar/resource_type/c_db06NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10486/696376reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6963762026-06-23T12:46:27Z
dc.title.none.fl_str_mv Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts
Utilización de materiales quitinolíticos para la obtención de oligosacáridos bioactivos. Caracterización de biocatalizadores y productos
title Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts
spellingShingle Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts
Kidibule, Peter Elias
Oligosacáridos
Enzimas
Biopolímeros
Biología y Biomedicina / Biología
title_short Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts
title_full Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts
title_fullStr Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts
title_full_unstemmed Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts
title_sort Using chitinolytic materials to obtain bioactive oligosaccharides. Characterization of biocatalysts
dc.creator.none.fl_str_mv Kidibule, Peter Elias
author Kidibule, Peter Elias
author_facet Kidibule, Peter Elias
author_role author
dc.contributor.none.fl_str_mv Fernández Lobato, María
Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Oligosacáridos
Enzimas
Biopolímeros
Biología y Biomedicina / Biología
topic Oligosacáridos
Enzimas
Biopolímeros
Biología y Biomedicina / Biología
description Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 14-04-2021
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-04-14
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/696376
url http://hdl.handle.net/10486/696376
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403065311821824
score 15.301603

Similares en LA Referencia