The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis

[EN] Living organisms have developed finely regulated homeostatic networks to mitigate the effects of environmental fluctuations in transition metal micronutrients, including iron, zinc, and copper. In Saccharomyces cerevisiae, , the tandem zinc-finger protein Cth2 post-transcriptionally regulates g...

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Autores: Andrés-Bordería, Amparo, Mazuque-Pons, L., Romeu-Perales, M., Garcia-Molina, Antoni, Martínez Pastor, María Teresa, Sanz, Amparo, Puig, Sergi, Peñarrubia, L., Pérez-García, Ana, Andrés-Colás, Nuria|||0000-0002-1455-2119
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/209528
Acceso en línea:https://riunet.upv.es/handle/10251/209528
Access Level:acceso abierto
Palabra clave:Arabidopsis thaliana
ARE
Copper
Post-transcriptional regulation
Zinc
BIOQUIMICA Y BIOLOGIA MOLECULAR
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oai_identifier_str oai:riunet.upv.es:10251/209528
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network_name_str España
repository_id_str
dc.title.none.fl_str_mv The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
title The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
spellingShingle The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
Andrés-Bordería, Amparo
Arabidopsis thaliana
ARE
Copper
Post-transcriptional regulation
Zinc
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
title_full The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
title_fullStr The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
title_full_unstemmed The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
title_sort The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
dc.creator.none.fl_str_mv Andrés-Bordería, Amparo
Mazuque-Pons, L.
Romeu-Perales, M.
Garcia-Molina, Antoni
Martínez Pastor, María Teresa
Sanz, Amparo
Puig, Sergi
Peñarrubia, L.
Pérez-García, Ana
Andrés-Colás, Nuria|||0000-0002-1455-2119
author Andrés-Bordería, Amparo
author_facet Andrés-Bordería, Amparo
Mazuque-Pons, L.
Romeu-Perales, M.
Garcia-Molina, Antoni
Martínez Pastor, María Teresa
Sanz, Amparo
Puig, Sergi
Peñarrubia, L.
Pérez-García, Ana
Andrés-Colás, Nuria|||0000-0002-1455-2119
author_role author
author2 Mazuque-Pons, L.
Romeu-Perales, M.
Garcia-Molina, Antoni
Martínez Pastor, María Teresa
Sanz, Amparo
Puig, Sergi
Peñarrubia, L.
Pérez-García, Ana
Andrés-Colás, Nuria|||0000-0002-1455-2119
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Generalitat Valenciana
Agencia Estatal de Investigación
Ministerio de Ciencia e Innovación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Arabidopsis thaliana
ARE
Copper
Post-transcriptional regulation
Zinc
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic Arabidopsis thaliana
ARE
Copper
Post-transcriptional regulation
Zinc
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] Living organisms have developed finely regulated homeostatic networks to mitigate the effects of environmental fluctuations in transition metal micronutrients, including iron, zinc, and copper. In Saccharomyces cerevisiae, , the tandem zinc-finger protein Cth2 post-transcriptionally regulates gene expression under conditions of iron deficiency by controlling the levels of mRNAs that code for non-essential ferroproteins. The molecular mechanism involves Cth2 binding to AU-rich elements present in the 3 ' ' untranslated region of target mRNAs, negatively affecting their stability and translation. Arabidopsis thaliana has two TZF proteins homologous to yeast Cth2, C3H14 and C3H15, which participate in cell wall remodelling. The present work examines the expression of representative metal homeostasis genes with putative AREs in plants with altered levels of C3H14 and C3H15 grown under varying metal availabilities. The results suggest that C3H15 may act as a post-transcriptional plant modulator of metal adequacy, as evidenced by the expression of SPL7, , the main transcriptional regulator under copper deficiency, and PETE2, , which encodes plastocyanin. In contrast to S. cerevisiae, , the plant C3H15 affects copper and zinc homeostasis rather than iron. When grown under copper-deficient conditions, adult C3H15OE OE plants exhibit lower chlorophyll content and photosynthetic efficiency compared to control plants, suggesting accelerated senescence. Likewise, metal content in C3H15OE(OE) plants under copper deficiency shows altered mobilization of copper and zinc to seeds. These data suggest that the C3H15 protein plays a role in modulating both cell wall remodelling and metal homeostasis. The interaction between these processes may be the cause of altered metal translocation.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-11-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/209528
url https://riunet.upv.es/handle/10251/209528
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-116940RB-I00 MECANISMOS DE ADAPTACION A CONDICIONES NO OPTIMAS DE COBRE Y HIERRO EN LEVADURAS Y PLANTAS
Generalitat Valenciana https://doi.org/10.13039/501100003359 CIGE%2F2021%2F092
Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 PID2023-148124OB-I00
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
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repository.mail.fl_str_mv
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spelling The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasisAndrés-Bordería, AmparoMazuque-Pons, L.Romeu-Perales, M.Garcia-Molina, AntoniMartínez Pastor, María TeresaSanz, AmparoPuig, SergiPeñarrubia, L.Pérez-García, AnaAndrés-Colás, Nuria|||0000-0002-1455-2119Arabidopsis thalianaARECopperPost-transcriptional regulationZincBIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Living organisms have developed finely regulated homeostatic networks to mitigate the effects of environmental fluctuations in transition metal micronutrients, including iron, zinc, and copper. In Saccharomyces cerevisiae, , the tandem zinc-finger protein Cth2 post-transcriptionally regulates gene expression under conditions of iron deficiency by controlling the levels of mRNAs that code for non-essential ferroproteins. The molecular mechanism involves Cth2 binding to AU-rich elements present in the 3 ' ' untranslated region of target mRNAs, negatively affecting their stability and translation. Arabidopsis thaliana has two TZF proteins homologous to yeast Cth2, C3H14 and C3H15, which participate in cell wall remodelling. The present work examines the expression of representative metal homeostasis genes with putative AREs in plants with altered levels of C3H14 and C3H15 grown under varying metal availabilities. The results suggest that C3H15 may act as a post-transcriptional plant modulator of metal adequacy, as evidenced by the expression of SPL7, , the main transcriptional regulator under copper deficiency, and PETE2, , which encodes plastocyanin. In contrast to S. cerevisiae, , the plant C3H15 affects copper and zinc homeostasis rather than iron. When grown under copper-deficient conditions, adult C3H15OE OE plants exhibit lower chlorophyll content and photosynthetic efficiency compared to control plants, suggesting accelerated senescence. Likewise, metal content in C3H15OE(OE) plants under copper deficiency shows altered mobilization of copper and zinc to seeds. These data suggest that the C3H15 protein plays a role in modulating both cell wall remodelling and metal homeostasis. The interaction between these processes may be the cause of altered metal translocation.We acknowledge Dr Patricia Casino for her help with the overlapping of the TZF domains, Dr Zhou for the C3H14, C3H15 overexpressing seeds and c3h14c3h15 (+/-) +/-) mutant and Marta Olmos for her technical support. A. Sanz obtained a sabbatical year grant (UV-PDI_SAB-156039). We acknowledge the SCSIE (Universitat de Valencia) for the ICP-MS service. This research was funded by the Spanish Ministry of Economy and Competitiveness "PID2020-116940RB-I00 and PID2023-148124OB-I00 funded by MCIN/AEI/10.13039/501100011033" and CIGE/2021/092 funded by Generalitat Valenciana.ElsevierInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalGeneralitat ValencianaAgencia Estatal de InvestigaciónMinisterio de Ciencia e InnovaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-11-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/209528reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-116940RB-I00 MECANISMOS DE ADAPTACION A CONDICIONES NO OPTIMAS DE COBRE Y HIERRO EN LEVADURAS Y PLANTASGeneralitat Valenciana https://doi.org/10.13039/501100003359 CIGE%2F2021%2F092Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 PID2023-148124OB-I00open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2095282026-06-13T07:49:27Z
score 15,81155

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