Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain

Background: Platanus acerifolia (London plane tree) is a deciduous tree of the Platanaceae family. Sensitization to this plant varies with geography. Madrid, located in central Spain, has one of the highest London plane tree pollen concentration levels on the Iberian Peninsula. Objectives: We evalua...

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Autores: Nuñez Borque, E., Betancor, D., Fernández Bravo, S., Gómez Cardeñosa, A., Esteban, V., Garrido Arandia, M., de las Heras, M., Pastor Vargas, Carlos, Cuesta Herranz, J.
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/109680
Acceso en línea:https://hdl.handle.net/20.500.14352/109680
Access Level:acceso abierto
Palabra clave:577.1
Plane tree
Platanus acerifolia
Allergen
Pollen allergy
Pla a 2
Alergología
Bioquímica (Química)
3207.01 Alergias
2302 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/109680
network_acronym_str ES
network_name_str España
repository_id_str
spelling Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central SpainNuñez Borque, E.Betancor, D.Fernández Bravo, S.Gómez Cardeñosa, A.Esteban, V.Garrido Arandia, M.de las Heras, M.Pastor Vargas, CarlosCuesta Herranz, J.577.1Plane treePlatanus acerifoliaAllergenPollen allergyPla a 2AlergologíaBioquímica (Química)3207.01 Alergias2302 BioquímicaBackground: Platanus acerifolia (London plane tree) is a deciduous tree of the Platanaceae family. Sensitization to this plant varies with geography. Madrid, located in central Spain, has one of the highest London plane tree pollen concentration levels on the Iberian Peninsula. Objectives: We evaluated both the clinical characteristics and the molecular sensitization pattern of patients with allergy to London plane tree pollen in the region of Madrid. Patients and Methods: Thirty-eight patients allergic to London plane tree pollen were selected according to their clinical symptoms and positive results in skin prick testing and/or specific IgE determination. Serum was collected, and allergen components were evaluated using immunodetection techniques as well as ImmunoCAP. The IgE-binding proteins detected were identified and characterized using mass spectrometry. Results: Analysis of serum samples from allergic patients revealed 9 IgE-binding bands in London plane tree pollen extract. Among these, the 45-kDa protein, which corresponded to Pla a 2, was detected in 76.3% of patients. However, the 18-kDa (Pla a 1) and 9-kDa (Pla a 3) bands were detected in 44.7% and 23.7% of sera, respectively. These results were confirmed using purified proteins. Characterization of the allergen revealed the 27-kDa protein to be glutathione-S-transferase. Conclusions: The molecular profile of patients sensitized to London plane tree pollen differs from that reported in studies from other locations. In the population we studied, the prevalence of Pla a 2 was higher than that of Pla a 1 and Pla a 3. In addition, the minor allergen previously referred to as Pla a 4 was characterized as glutathione-S-transferase.EsmonUniversidad Complutense de Madrid20222022-10-1020222022-10-10journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/109680reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available RD16%2F0006%2F0035 Asma, Reacciones Adversas y Alérgicas (ARADYAL)Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available RD16%2F0006%2F0014 Asma, Reacciones Adversas y Alérgicas (ARADYAL)open accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1096802026-06-02T12:44:21Z
dc.title.none.fl_str_mv Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain
title Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain
spellingShingle Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain
Nuñez Borque, E.
577.1
Plane tree
Platanus acerifolia
Allergen
Pollen allergy
Pla a 2
Alergología
Bioquímica (Química)
3207.01 Alergias
2302 Bioquímica
title_short Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain
title_full Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain
title_fullStr Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain
title_full_unstemmed Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain
title_sort Allergen Profile of London Plane Tree Pollen: Clinical and Molecular Pattern in Central Spain
dc.creator.none.fl_str_mv Nuñez Borque, E.
Betancor, D.
Fernández Bravo, S.
Gómez Cardeñosa, A.
Esteban, V.
Garrido Arandia, M.
de las Heras, M.
Pastor Vargas, Carlos
Cuesta Herranz, J.
author Nuñez Borque, E.
author_facet Nuñez Borque, E.
Betancor, D.
Fernández Bravo, S.
Gómez Cardeñosa, A.
Esteban, V.
Garrido Arandia, M.
de las Heras, M.
Pastor Vargas, Carlos
Cuesta Herranz, J.
author_role author
author2 Betancor, D.
Fernández Bravo, S.
Gómez Cardeñosa, A.
Esteban, V.
Garrido Arandia, M.
de las Heras, M.
Pastor Vargas, Carlos
Cuesta Herranz, J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.1
Plane tree
Platanus acerifolia
Allergen
Pollen allergy
Pla a 2
Alergología
Bioquímica (Química)
3207.01 Alergias
2302 Bioquímica
topic 577.1
Plane tree
Platanus acerifolia
Allergen
Pollen allergy
Pla a 2
Alergología
Bioquímica (Química)
3207.01 Alergias
2302 Bioquímica
description Background: Platanus acerifolia (London plane tree) is a deciduous tree of the Platanaceae family. Sensitization to this plant varies with geography. Madrid, located in central Spain, has one of the highest London plane tree pollen concentration levels on the Iberian Peninsula. Objectives: We evaluated both the clinical characteristics and the molecular sensitization pattern of patients with allergy to London plane tree pollen in the region of Madrid. Patients and Methods: Thirty-eight patients allergic to London plane tree pollen were selected according to their clinical symptoms and positive results in skin prick testing and/or specific IgE determination. Serum was collected, and allergen components were evaluated using immunodetection techniques as well as ImmunoCAP. The IgE-binding proteins detected were identified and characterized using mass spectrometry. Results: Analysis of serum samples from allergic patients revealed 9 IgE-binding bands in London plane tree pollen extract. Among these, the 45-kDa protein, which corresponded to Pla a 2, was detected in 76.3% of patients. However, the 18-kDa (Pla a 1) and 9-kDa (Pla a 3) bands were detected in 44.7% and 23.7% of sera, respectively. These results were confirmed using purified proteins. Characterization of the allergen revealed the 27-kDa protein to be glutathione-S-transferase. Conclusions: The molecular profile of patients sensitized to London plane tree pollen differs from that reported in studies from other locations. In the population we studied, the prevalence of Pla a 2 was higher than that of Pla a 1 and Pla a 3. In addition, the minor allergen previously referred to as Pla a 4 was characterized as glutathione-S-transferase.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-10-10
2022
2022-10-10
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/109680
url https://hdl.handle.net/20.500.14352/109680
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available RD16%2F0006%2F0035 Asma, Reacciones Adversas y Alérgicas (ARADYAL)
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available RD16%2F0006%2F0014 Asma, Reacciones Adversas y Alérgicas (ARADYAL)
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Esmon
publisher.none.fl_str_mv Esmon
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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