Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis

Otx1 belongs to the paired class of homeobox genes and plays a pivotal role in brain development. Here, we show that Otx1 is expressed in hematopoietic pluripotent and erythroid progenitor cells. Moreover, bone marrow cells from mice lacking Otx1 exhibit a cell-autonomous impairment of the erythroid...

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Autores: Levantini, Elena, Giorgetti, Alessandra, Cerisoli, Francesco, Traggiai, Elisabetta, Guidi, Alessandra, Martin, Richard, Acampora, Dario, Aplan, Peter D., Keller, Gordon, Simeone, Antonio, Iscove, Norman N., Hoang, Trang, Magli, Maria Cristina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/176544
Acceso en línea:https://hdl.handle.net/2445/176544
Access Level:acceso abierto
Palabra clave:Hematopoesi
Fisiologia
Factors de transcripció
Hematopoiesis
Physiology
Transcription factors
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spelling Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesisLevantini, ElenaGiorgetti, AlessandraCerisoli, FrancescoTraggiai, ElisabettaGuidi, AlessandraMartin, RichardAcampora, DarioAplan, Peter D.Keller, GordonSimeone, AntonioIscove, Norman N.Hoang, TrangMagli, Maria CristinaHematopoesiFisiologiaFactors de transcripcióHematopoiesisPhysiologyTranscription factorsOtx1 belongs to the paired class of homeobox genes and plays a pivotal role in brain development. Here, we show that Otx1 is expressed in hematopoietic pluripotent and erythroid progenitor cells. Moreover, bone marrow cells from mice lacking Otx1 exhibit a cell-autonomous impairment of the erythroid compartment. In agreement with these results, molecular analysis revealed decreased levels of erythroid genes that include the SCL and GATA-1 transcription factors. Accordingly, a gain of function of SCL rescues the erythroid deficiency in Otx1-/- mice. Taken together, our findings indicate a function for Otx1 in the regulation of blood cell production. There is growing evidence suggesting that common cellular and molecular mechanisms orchestrate differentiation in various tissues. Homeobox-containing genes seem to be strong candidate genes to regulate a number of developmental processes, including neurogenesis and hematopoiesis. Members of the Otx family (Otx1, Otx2, Otx3, and Crx) are the vertebrate homologues of the Drosophila head gap gene orthodenticle and encode transcription factors containing a bicoid-like homeodomain. They are temporally and spatially regulated during development and seem to be required for proper head and sense organ patterning. Otx1, Otx2, and Otx3 show partially overlapping, but distinct expression patterns, and Otx2, the first to be activated during development, plays a major role in gastrulation and in the early specification of the anterior neural plate. In contrast, Otx1 shows a later onset and is involved in corticogenesis, sense organ development, and pituitary function. Mice bearing targeted deletion of Otx1 are affected by a permanent epileptic phenotype and show multiple brain abnormalities and morphological defects of the acoustic and visual sense organs. In addition, at the prepubescent stage, they exhibit transient dwarfism and hypogonadism because of low levels of pituitary hormones. In the present study, we have investigated whether Otx1 also plays a role in blood cell production, as several homeobox genes of different families are involved in normal and/or malignant hematopoiesis.National Academy of Sciences2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/176544Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://www.pnas.org/content/100/18Proceedings of the National Academy of Sciences of the United States of America - PNAS, 2003, vol. 100, num. 18, p. 10299-10303(c) National Academy of Sciences, 2003info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1765442026-05-27T06:46:51Z
dc.title.none.fl_str_mv Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis
title Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis
spellingShingle Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis
Levantini, Elena
Hematopoesi
Fisiologia
Factors de transcripció
Hematopoiesis
Physiology
Transcription factors
title_short Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis
title_full Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis
title_fullStr Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis
title_full_unstemmed Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis
title_sort Unsuspected role of the brain morphogenetic gene Otx1 in hematopoiesis
dc.creator.none.fl_str_mv Levantini, Elena
Giorgetti, Alessandra
Cerisoli, Francesco
Traggiai, Elisabetta
Guidi, Alessandra
Martin, Richard
Acampora, Dario
Aplan, Peter D.
Keller, Gordon
Simeone, Antonio
Iscove, Norman N.
Hoang, Trang
Magli, Maria Cristina
author Levantini, Elena
author_facet Levantini, Elena
Giorgetti, Alessandra
Cerisoli, Francesco
Traggiai, Elisabetta
Guidi, Alessandra
Martin, Richard
Acampora, Dario
Aplan, Peter D.
Keller, Gordon
Simeone, Antonio
Iscove, Norman N.
Hoang, Trang
Magli, Maria Cristina
author_role author
author2 Giorgetti, Alessandra
Cerisoli, Francesco
Traggiai, Elisabetta
Guidi, Alessandra
Martin, Richard
Acampora, Dario
Aplan, Peter D.
Keller, Gordon
Simeone, Antonio
Iscove, Norman N.
Hoang, Trang
Magli, Maria Cristina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hematopoesi
Fisiologia
Factors de transcripció
Hematopoiesis
Physiology
Transcription factors
topic Hematopoesi
Fisiologia
Factors de transcripció
Hematopoiesis
Physiology
Transcription factors
description Otx1 belongs to the paired class of homeobox genes and plays a pivotal role in brain development. Here, we show that Otx1 is expressed in hematopoietic pluripotent and erythroid progenitor cells. Moreover, bone marrow cells from mice lacking Otx1 exhibit a cell-autonomous impairment of the erythroid compartment. In agreement with these results, molecular analysis revealed decreased levels of erythroid genes that include the SCL and GATA-1 transcription factors. Accordingly, a gain of function of SCL rescues the erythroid deficiency in Otx1-/- mice. Taken together, our findings indicate a function for Otx1 in the regulation of blood cell production. There is growing evidence suggesting that common cellular and molecular mechanisms orchestrate differentiation in various tissues. Homeobox-containing genes seem to be strong candidate genes to regulate a number of developmental processes, including neurogenesis and hematopoiesis. Members of the Otx family (Otx1, Otx2, Otx3, and Crx) are the vertebrate homologues of the Drosophila head gap gene orthodenticle and encode transcription factors containing a bicoid-like homeodomain. They are temporally and spatially regulated during development and seem to be required for proper head and sense organ patterning. Otx1, Otx2, and Otx3 show partially overlapping, but distinct expression patterns, and Otx2, the first to be activated during development, plays a major role in gastrulation and in the early specification of the anterior neural plate. In contrast, Otx1 shows a later onset and is involved in corticogenesis, sense organ development, and pituitary function. Mice bearing targeted deletion of Otx1 are affected by a permanent epileptic phenotype and show multiple brain abnormalities and morphological defects of the acoustic and visual sense organs. In addition, at the prepubescent stage, they exhibit transient dwarfism and hypogonadism because of low levels of pituitary hormones. In the present study, we have investigated whether Otx1 also plays a role in blood cell production, as several homeobox genes of different families are involved in normal and/or malignant hematopoiesis.
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/176544
url https://hdl.handle.net/2445/176544
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://www.pnas.org/content/100/18
Proceedings of the National Academy of Sciences of the United States of America - PNAS, 2003, vol. 100, num. 18, p. 10299-10303
dc.rights.none.fl_str_mv (c) National Academy of Sciences, 2003
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) National Academy of Sciences, 2003
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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