DNA methylation in plants: mechanisms and tools for targeted manipulation.

[EN] DNA methylation is an epigenetic mark that regulates multiple processes, such as gene expression and genome stability. Mutants and pharmacological treatments have been instrumental in the study of this mark in plants, although their genome-wide effect complicates the direct association between...

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Detalles Bibliográficos
Autor: Gallego Bartolomé, Javier
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/201043
Acceso en línea:https://riunet.upv.es/handle/10251/201043
Access Level:acceso abierto
Palabra clave:Artificial zinc finger proteins
CRISPR SunTag
DNA methylation
RNA-directed DNA methylation
Transcriptional gene silencing
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spelling DNA methylation in plants: mechanisms and tools for targeted manipulation.Gallego Bartolomé, JavierArtificial zinc finger proteinsCRISPR SunTagDNA methylationRNA-directed DNA methylationTranscriptional gene silencing[EN] DNA methylation is an epigenetic mark that regulates multiple processes, such as gene expression and genome stability. Mutants and pharmacological treatments have been instrumental in the study of this mark in plants, although their genome-wide effect complicates the direct association between changes in methylation and a particular phenotype. A variety of tools that allow locus-specific manipulation of DNA methylation can be used to assess its direct role in specific processes, as well as to create novel epialleles. Recently, new tools that recruit the methylation machinery directly to target loci through programmable DNA-binding proteins have expanded the tool kit available to researchers. This review provides an overview of DNA methylation in plants and discusses the tools that have recently been developed for its manipulation.I would like to thank Drs Steve Jacobsen, Jake Harris, German Martinez, Maria A. Nohales, Miguel Blazquez and David Alabadi, as well as three anonymous reviewers for their critical reading of this manuscript and insightful comments. I would also like to apologize to colleagues whose work is not included in this manuscript due to space limitations. JG-B is funded by a Marie Curie Reintegration grant (MSCA-IF EU project 835599).Blackwell PublishingEuropean CommissionRepositorio Institucional de la Universitat Politècnica de València Riunet20202020-07-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/201043reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 H2020 835599open accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2010432026-06-13T07:49:27Z
dc.title.none.fl_str_mv DNA methylation in plants: mechanisms and tools for targeted manipulation.
title DNA methylation in plants: mechanisms and tools for targeted manipulation.
spellingShingle DNA methylation in plants: mechanisms and tools for targeted manipulation.
Gallego Bartolomé, Javier
Artificial zinc finger proteins
CRISPR SunTag
DNA methylation
RNA-directed DNA methylation
Transcriptional gene silencing
title_short DNA methylation in plants: mechanisms and tools for targeted manipulation.
title_full DNA methylation in plants: mechanisms and tools for targeted manipulation.
title_fullStr DNA methylation in plants: mechanisms and tools for targeted manipulation.
title_full_unstemmed DNA methylation in plants: mechanisms and tools for targeted manipulation.
title_sort DNA methylation in plants: mechanisms and tools for targeted manipulation.
dc.creator.none.fl_str_mv Gallego Bartolomé, Javier
author Gallego Bartolomé, Javier
author_facet Gallego Bartolomé, Javier
author_role author
dc.contributor.none.fl_str_mv European Commission
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Artificial zinc finger proteins
CRISPR SunTag
DNA methylation
RNA-directed DNA methylation
Transcriptional gene silencing
topic Artificial zinc finger proteins
CRISPR SunTag
DNA methylation
RNA-directed DNA methylation
Transcriptional gene silencing
description [EN] DNA methylation is an epigenetic mark that regulates multiple processes, such as gene expression and genome stability. Mutants and pharmacological treatments have been instrumental in the study of this mark in plants, although their genome-wide effect complicates the direct association between changes in methylation and a particular phenotype. A variety of tools that allow locus-specific manipulation of DNA methylation can be used to assess its direct role in specific processes, as well as to create novel epialleles. Recently, new tools that recruit the methylation machinery directly to target loci through programmable DNA-binding proteins have expanded the tool kit available to researchers. This review provides an overview of DNA methylation in plants and discusses the tools that have recently been developed for its manipulation.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-07-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/201043
url https://riunet.upv.es/handle/10251/201043
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 H2020 835599
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reserva de todos los derechos
http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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