Solea senegalensis sperm cryopreservation: New insights on sperm quality

[EN] Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It...

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Detalles Bibliográficos
Autores: Fernández Riesco, Marta, Oliveira, Catarina, Soares, Florbela, Gavaia, Paulo J., Dinis, María T., Cabrita, Elsa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Ajuntament de Barcelona
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/21700
Acceso en línea:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0186542
http://hdl.handle.net/10612/21700
Access Level:acceso abierto
Palabra clave:Biología
Bilogía molecular
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spelling Solea senegalensis sperm cryopreservation: New insights on sperm qualityFernández Riesco, MartaOliveira, CatarinaSoares, FlorbelaGavaia, Paulo J.Dinis, María T.Cabrita, ElsaBiologíaBilogía molecular[EN] Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation.SIPROMAR ProgramPublic Library of ScienceBiologia CelularFacultad de Ciencias de la Salud2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0186542http://hdl.handle.net/10612/21700reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Ajuntament de BarcelonaInglésREPLING 31-03-05-FEP-69KLING 31-03-05- FEP-73http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/217002026-06-24T12:43:27Z
dc.title.none.fl_str_mv Solea senegalensis sperm cryopreservation: New insights on sperm quality
title Solea senegalensis sperm cryopreservation: New insights on sperm quality
spellingShingle Solea senegalensis sperm cryopreservation: New insights on sperm quality
Fernández Riesco, Marta
Biología
Bilogía molecular
title_short Solea senegalensis sperm cryopreservation: New insights on sperm quality
title_full Solea senegalensis sperm cryopreservation: New insights on sperm quality
title_fullStr Solea senegalensis sperm cryopreservation: New insights on sperm quality
title_full_unstemmed Solea senegalensis sperm cryopreservation: New insights on sperm quality
title_sort Solea senegalensis sperm cryopreservation: New insights on sperm quality
dc.creator.none.fl_str_mv Fernández Riesco, Marta
Oliveira, Catarina
Soares, Florbela
Gavaia, Paulo J.
Dinis, María T.
Cabrita, Elsa
author Fernández Riesco, Marta
author_facet Fernández Riesco, Marta
Oliveira, Catarina
Soares, Florbela
Gavaia, Paulo J.
Dinis, María T.
Cabrita, Elsa
author_role author
author2 Oliveira, Catarina
Soares, Florbela
Gavaia, Paulo J.
Dinis, María T.
Cabrita, Elsa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Biologia Celular
Facultad de Ciencias de la Salud
dc.subject.none.fl_str_mv Biología
Bilogía molecular
topic Biología
Bilogía molecular
description [EN] Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed. In the present study, motility and lipid peroxidation were performed as specific tests allowing us to discard cryopreservation conditions such as methanol as internal cryoprotectant and bovine serum albumin as external cryoprotectant. In addition, a caspase 3/7 detection by flow cytometry was performed to analyze apoptosis activity in the best selected conditions. Moreover, new highly sensitive tests based on transcript number detection have recently been described in fish sperm cryopreservation. For this reason, a transcript level detection assay was performed on certain oxidative and chaperone genes related to fertilization ability and embryo development (hsp70, hsp90BB, hsp90AA, gpx) to select the best cryopreservation conditions. DMSO+ egg yolk proved to be the best cryoprotectant combination in terms of transcript level. This study describes an optimized cryopreservation protocol for Solea senegalensis sperm demonstrating for the first time that transcript degradation is the most sensitive predictor of cell status in this species after cryopreservation.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0186542
http://hdl.handle.net/10612/21700
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0186542
http://hdl.handle.net/10612/21700
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv REPLING 31-03-05-FEP-69
KLING 31-03-05- FEP-73
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Ajuntament de Barcelona
instname_str Ajuntament de Barcelona
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
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repository.mail.fl_str_mv
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