Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms

64 páginas, 7 figuras, 5 tablas, 4 figuras suplementarias, 3 tablas suplementarias. -- The definitve version is available at: http://www.elsevier.com

Bibliographic Details
Authors: Obolo Mvoulouga, Prosper, Oleaga, Ana, Manzano Román, Raúl, Pérez Sánchez, Ricardo
Format: article
Status:Versión aceptada para publicación
Publication Date:2018
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/165401
Online Access:http://hdl.handle.net/10261/165401
Access Level:Open access
Keyword:Ornithodoros moubata
Mialome
Vaccines
Midgut antigens
Recombinant antigens
Synthetic peptides
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spelling Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithmsObolo Mvoulouga, ProsperOleaga, AnaManzano Román, RaúlPérez Sánchez, RicardoOrnithodoros moubataMialomeVaccinesMidgut antigensRecombinant antigensSynthetic peptides64 páginas, 7 figuras, 5 tablas, 4 figuras suplementarias, 3 tablas suplementarias. -- The definitve version is available at: http://www.elsevier.comThe African argasid tick Ornithodoros moubata transmits two important pathogens, the African swine fever virus and the spirochete Borrelia duttoni, the cause of human relapsing fever. To date, only conventional control measures such as widespread application of acaricides, strict control measures, and animal movement restrictions have been implemented to confine these diseases. Vaccines against tick infestations have the potential to be among the most efficacious interventions for the management of these diseases. Plasma membrane-associated proteins upregulated in tick midgut cells in response to blood feeding and digestion are thought to play vital functions in tick physiology and in the transmission of tick-borne pathogens. In addition, their antigenic extracellular regions are easily accessible to antibodies synthesised by immunised hosts, which makes them interesting targets for tick vaccine design. The mialomes (midgut transcriptomes and proteomes) of unfed O. moubata females and of engorged females at 48 h post-feeding have recently been obtained, providing a wealth of predicted midgut protein sequences. In the current study, these mialomes were screened using in silico tools to select predicted antigenic transmembrane proteins that were upregulated after feeding (516 proteins). The functionally annotatable proteins from this list (396 proteins) were then manually inspected following additional criteria in order to select a finite and easy-manageable number of candidate antigens for tick vaccine design. The extracellular antigenic regions of five of these candidates were obtained either as truncated recombinant proteins or as KLH-conjugated synthetic peptides, formulated in Freund’s adjuvant, and individually administered to rabbits to assess their immunogenicity and protective potential against infestations by O. moubata and the Iberian species Ornithodoros erraticus. All candidates were highly immunogenic, but provided low protection against the O. moubata infestations (ranging from 7% to 39%). Interestingly, all candidates except one also protected against infestations by O. erraticus, achieving higher efficacies against this species (from 20% to 66%). According to their protective potential, three of the five antigens tested (Om17, Om86 and OM99) were considered little suitable for use in tick vaccines, while the other two (OM85 and OM03) were considered useful antigens for tick vaccine development, deserving further studies.This work was supported by project AGL2013–42745-P funded by a grant from the Spanish Ministry of Economy and Competitiveness.Peer reviewedElsevierMinisterio de Economía, Industria y Competitividad (España)Oleaga, Ana [0000-0002-8019-7354]Manzano Román, Raúl [0000-0002-0165-2604]Pérez Sánchez, Ricardo [0000-0001-8875-439X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/165401reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-42745-Phttps://doi.org/10.1016/j.ttbdis.2018.04.015Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1654012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms
title Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms
spellingShingle Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms
Obolo Mvoulouga, Prosper
Ornithodoros moubata
Mialome
Vaccines
Midgut antigens
Recombinant antigens
Synthetic peptides
title_short Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms
title_full Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms
title_fullStr Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms
title_full_unstemmed Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms
title_sort Evaluation of the protective efficacy of Ornithodoros moubata midgut membrane antigens selected using omics and in silico prediction algorithms
dc.creator.none.fl_str_mv Obolo Mvoulouga, Prosper
Oleaga, Ana
Manzano Román, Raúl
Pérez Sánchez, Ricardo
author Obolo Mvoulouga, Prosper
author_facet Obolo Mvoulouga, Prosper
Oleaga, Ana
Manzano Román, Raúl
Pérez Sánchez, Ricardo
author_role author
author2 Oleaga, Ana
Manzano Román, Raúl
Pérez Sánchez, Ricardo
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
Oleaga, Ana [0000-0002-8019-7354]
Manzano Román, Raúl [0000-0002-0165-2604]
Pérez Sánchez, Ricardo [0000-0001-8875-439X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ornithodoros moubata
Mialome
Vaccines
Midgut antigens
Recombinant antigens
Synthetic peptides
topic Ornithodoros moubata
Mialome
Vaccines
Midgut antigens
Recombinant antigens
Synthetic peptides
description 64 páginas, 7 figuras, 5 tablas, 4 figuras suplementarias, 3 tablas suplementarias. -- The definitve version is available at: http://www.elsevier.com
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/165401
url http://hdl.handle.net/10261/165401
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-42745-P
https://doi.org/10.1016/j.ttbdis.2018.04.015
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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