Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies

<p><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Purpose:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> </span><em style="background-color:rgb( 255 , 255 , 255 );c...

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Autores: Carvajal Vidal, Paulina Andrea, Folle, Camila, Marqués Villavecchia, Ana M., Díaz-Garrido, Natalia, Sánchez-López, E. (Elena), Suñer Carbó, J. (Joaquim), Halbaut, Lyda, Mallandrich Miret, Mireia, Espina García, Marta, Badía Palacín, Josefa, Baldomà Llavinés, Laura, García López, María Luisa, Calpena Campmany, Ana Cristina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/207864
Acceso en línea:https://hdl.handle.net/2445/207864
Access Level:acceso abierto
Palabra clave:Gels (Farmàcia)
Emulsions (Farmàcia)
Nanopartícules
Gels (Pharmacy)
Emulsions (Pharmacy)
Nanoparticles
id ES_084f52a35eb05ffccdb27d854b4342aa
oai_identifier_str oai:diposit.ub.edu:2445/207864
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies
title Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies
spellingShingle Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies
Carvajal Vidal, Paulina Andrea
Gels (Farmàcia)
Emulsions (Farmàcia)
Nanopartícules
Gels (Pharmacy)
Emulsions (Pharmacy)
Nanoparticles
title_short Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies
title_full Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies
title_fullStr Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies
title_full_unstemmed Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies
title_sort Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal Pathologies
dc.creator.none.fl_str_mv Carvajal Vidal, Paulina Andrea
Folle, Camila
Marqués Villavecchia, Ana M.
Díaz-Garrido, Natalia
Sánchez-López, E. (Elena)
Suñer Carbó, J. (Joaquim)
Halbaut, Lyda
Mallandrich Miret, Mireia
Espina García, Marta
Badía Palacín, Josefa
Baldomà Llavinés, Laura
García López, María Luisa
Calpena Campmany, Ana Cristina
author Carvajal Vidal, Paulina Andrea
author_facet Carvajal Vidal, Paulina Andrea
Folle, Camila
Marqués Villavecchia, Ana M.
Díaz-Garrido, Natalia
Sánchez-López, E. (Elena)
Suñer Carbó, J. (Joaquim)
Halbaut, Lyda
Mallandrich Miret, Mireia
Espina García, Marta
Badía Palacín, Josefa
Baldomà Llavinés, Laura
García López, María Luisa
Calpena Campmany, Ana Cristina
author_role author
author2 Folle, Camila
Marqués Villavecchia, Ana M.
Díaz-Garrido, Natalia
Sánchez-López, E. (Elena)
Suñer Carbó, J. (Joaquim)
Halbaut, Lyda
Mallandrich Miret, Mireia
Espina García, Marta
Badía Palacín, Josefa
Baldomà Llavinés, Laura
García López, María Luisa
Calpena Campmany, Ana Cristina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gels (Farmàcia)
Emulsions (Farmàcia)
Nanopartícules
Gels (Pharmacy)
Emulsions (Pharmacy)
Nanoparticles
topic Gels (Farmàcia)
Emulsions (Farmàcia)
Nanopartícules
Gels (Pharmacy)
Emulsions (Pharmacy)
Nanoparticles
description <p><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Purpose:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> </span><em style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Acne vulgaris</em><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> is one of the most prevalent dermal disorders affecting skin health and appearance. To date, there is no effective cure for this pathology, and the majority of marketed formulations eliminate both healthy and pathological microbiota. Therefore, hereby we propose the encapsulation of an antimicrobial natural compound (thymol) loaded into lipid nanostructured systems to be topically used against acne.</span><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Methods:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> To address this issue, nanostructured lipid carriers (NLC) capable of encapsulating thymol, a natural compound used for the treatment of acne vulgaris, were developed either using ultrasonication probe or high-pressure homogenization and optimized using 2</span><sup style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">2</sup><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">-star factorial design by analyzing the effect of NLC composition on their physicochemical parameters. These NLC were optimized using a design of experiments approach and were characterized using different physicochemical techniques. Moreover, short-term stability and cell viability using HaCat cells were assessed. Antimicrobial efficacy of the developed NLC was assessed in vitro and ex vivo.</span><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Results:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> NLC encapsulating thymol were developed and optimized and demonstrated a prolonged thymol release. The formulation was dispersed in gels and a screening of several gels was carried out by studying their rheological properties and their skin retention abilities. From them, carbomer demonstrated the capacity to be highly retained in skin tissues, specifically in the epidermis and dermis layers. Moreover, antimicrobial assays against healthy and pathological skin pathogens demonstrated the therapeutic efficacy of thymol-loaded NLC gelling systems since NLC are more efficient in slowly reducing </span><em style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">C. acnes</em><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> viability, but they possess lower antimicrobial activity against </span><em style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">S. epidermidis</em><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">, compared to free thymol.</span><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Conclusion:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> Thymol was successfully loaded into NLC and dispersed in gelling systems, demonstrating that it is a suitable candidate for topical administration against acne vulgaris by eradicating pathogenic bacteria while preserving the healthy skin microbiome.</span></p>
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/207864
url https://hdl.handle.net/2445/207864
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.2147/IJN.S433686
International Journal of Nanomedicine, 2024, vol. 19, p. 1225-1248
https://doi.org/10.2147/IJN.S433686
dc.rights.none.fl_str_mv cc-by-nc (c) Folle C. et al., 2024
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Folle C. et al., 2024
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Dove Medical Press
publisher.none.fl_str_mv Dove Medical Press
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403035250196480
spelling Gel-Dispersed Nanostructures Lipid Carriers Loading Thymol Designed for Dermal PathologiesCarvajal Vidal, Paulina AndreaFolle, CamilaMarqués Villavecchia, Ana M.Díaz-Garrido, NataliaSánchez-López, E. (Elena) Suñer Carbó, J. (Joaquim)Halbaut, LydaMallandrich Miret, MireiaEspina García, MartaBadía Palacín, JosefaBaldomà Llavinés, LauraGarcía López, María LuisaCalpena Campmany, Ana CristinaGels (Farmàcia)Emulsions (Farmàcia)NanopartículesGels (Pharmacy)Emulsions (Pharmacy)Nanoparticles<p><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Purpose:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> </span><em style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Acne vulgaris</em><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> is one of the most prevalent dermal disorders affecting skin health and appearance. To date, there is no effective cure for this pathology, and the majority of marketed formulations eliminate both healthy and pathological microbiota. Therefore, hereby we propose the encapsulation of an antimicrobial natural compound (thymol) loaded into lipid nanostructured systems to be topically used against acne.</span><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Methods:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> To address this issue, nanostructured lipid carriers (NLC) capable of encapsulating thymol, a natural compound used for the treatment of acne vulgaris, were developed either using ultrasonication probe or high-pressure homogenization and optimized using 2</span><sup style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">2</sup><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">-star factorial design by analyzing the effect of NLC composition on their physicochemical parameters. These NLC were optimized using a design of experiments approach and were characterized using different physicochemical techniques. Moreover, short-term stability and cell viability using HaCat cells were assessed. Antimicrobial efficacy of the developed NLC was assessed in vitro and ex vivo.</span><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Results:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> NLC encapsulating thymol were developed and optimized and demonstrated a prolonged thymol release. The formulation was dispersed in gels and a screening of several gels was carried out by studying their rheological properties and their skin retention abilities. From them, carbomer demonstrated the capacity to be highly retained in skin tissues, specifically in the epidermis and dermis layers. Moreover, antimicrobial assays against healthy and pathological skin pathogens demonstrated the therapeutic efficacy of thymol-loaded NLC gelling systems since NLC are more efficient in slowly reducing </span><em style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">C. acnes</em><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> viability, but they possess lower antimicrobial activity against </span><em style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">S. epidermidis</em><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">, compared to free thymol.</span><strong style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )">Conclusion:</strong><span style="background-color:rgb( 255 , 255 , 255 );color:rgb( 85 , 85 , 85 )"> Thymol was successfully loaded into NLC and dispersed in gelling systems, demonstrating that it is a suitable candidate for topical administration against acne vulgaris by eradicating pathogenic bacteria while preserving the healthy skin microbiome.</span></p>Dove Medical Press2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/207864Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.2147/IJN.S433686International Journal of Nanomedicine, 2024, vol. 19, p. 1225-1248https://doi.org/10.2147/IJN.S433686cc-by-nc (c) Folle C. et al., 2024http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2078642026-05-27T06:46:51Z
score 15,300719

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