Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.

Context: Circulating microRNAs (miRNAs) are emerging biomarkers of thyroid cancer. Objective: This study sought to identify the profile of circulating miRNAs and its response to human recombinant TSH (rhTSH) in thyroid cancer patients with recurrent/persistent disease. Methods: We obtained serum sam...

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Detalhes bibliográficos
Autores: Jankovic Miljus, Jelena, Guillén-Sacoto, María Augusta, Makiadi-Alvarado, Jennifer, Wert-Lamas, León, Ramirez-Moya, Julia, Robledo, Mercedes, Santisteban, Pilar, Riesco Eizaguirre, Garcilaso
Tipo de documento: artigo
Data de publicação:2022
País:España
Recursos:Universidad Francisco de Vitoria
Repositório:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
Idioma:inglês
OAI Identifier:oai:ddfv.ufv.es:10641/3777
Acesso em linha:https://hdl.handle.net/10641/3777
Access Level:Acceso aberto
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spelling Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.Jankovic Miljus, JelenaGuillén-Sacoto, María AugustaMakiadi-Alvarado, JenniferWert-Lamas, LeónRamirez-Moya, JuliaRobledo, MercedesSantisteban, PilarRiesco Eizaguirre, GarcilasoContext: Circulating microRNAs (miRNAs) are emerging biomarkers of thyroid cancer. Objective: This study sought to identify the profile of circulating miRNAs and its response to human recombinant TSH (rhTSH) in thyroid cancer patients with recurrent/persistent disease. Methods: We obtained serum samples from 30 patients with differentiated thyroid cancer, 14 with recurrent/persistent disease and 16 with complete remission. We used next-generation sequencing to define the miRnomes along with a comprehensive quantitative PCR (qPCR) validation using 2 different platforms. We made a transversal study by comparing serum miRNA profiles of patients with or without recurrent/persistent disease and a longitudinal study looking at differences before and after rhTSH stimulation. Selected miRNAs were then studied in human thyroid cancer cell lines TPC-1, FTC-133, and OCUT-2 in response to TSH stimulation. Results: We could not demonstrate any consistent differences in serum profiles of known miRNAs between patients with and without recurrent/ persistent disease or before and after rhTSH stimulation. However, our sequencing data revealed 2 putative novel miRNAs that rise with rhTSH stimulation in the serums of patients with recurrent/persistent disease. We further confirmed by qPCR the upregulation of these putative miRNAs both in serums and in TSH-stimulated cells. We also show miRNAs that are good candidates for housekeeping genes in the serum of patients independently of the levels of TSH. Conclusions: The present study does not provide evidence that known miRNAs can be used as circulating markers for recurrence of thyroid cancer. However, we suggest that novel miRNA molecules may be related to thyroid cancer pathogenesis.20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10641/3777reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoriainstname:Universidad Francisco de VitoriaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ddfv.ufv.es:10641/37772026-06-11T12:44:57Z
dc.title.none.fl_str_mv Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.
title Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.
spellingShingle Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.
Jankovic Miljus, Jelena
title_short Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.
title_full Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.
title_fullStr Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.
title_full_unstemmed Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.
title_sort Circulating MicroRNA Profiles as Potential Biomarkers for Differentiated Thyroid Cancer Recurrence.
dc.creator.none.fl_str_mv Jankovic Miljus, Jelena
Guillén-Sacoto, María Augusta
Makiadi-Alvarado, Jennifer
Wert-Lamas, León
Ramirez-Moya, Julia
Robledo, Mercedes
Santisteban, Pilar
Riesco Eizaguirre, Garcilaso
author Jankovic Miljus, Jelena
author_facet Jankovic Miljus, Jelena
Guillén-Sacoto, María Augusta
Makiadi-Alvarado, Jennifer
Wert-Lamas, León
Ramirez-Moya, Julia
Robledo, Mercedes
Santisteban, Pilar
Riesco Eizaguirre, Garcilaso
author_role author
author2 Guillén-Sacoto, María Augusta
Makiadi-Alvarado, Jennifer
Wert-Lamas, León
Ramirez-Moya, Julia
Robledo, Mercedes
Santisteban, Pilar
Riesco Eizaguirre, Garcilaso
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
description Context: Circulating microRNAs (miRNAs) are emerging biomarkers of thyroid cancer. Objective: This study sought to identify the profile of circulating miRNAs and its response to human recombinant TSH (rhTSH) in thyroid cancer patients with recurrent/persistent disease. Methods: We obtained serum samples from 30 patients with differentiated thyroid cancer, 14 with recurrent/persistent disease and 16 with complete remission. We used next-generation sequencing to define the miRnomes along with a comprehensive quantitative PCR (qPCR) validation using 2 different platforms. We made a transversal study by comparing serum miRNA profiles of patients with or without recurrent/persistent disease and a longitudinal study looking at differences before and after rhTSH stimulation. Selected miRNAs were then studied in human thyroid cancer cell lines TPC-1, FTC-133, and OCUT-2 in response to TSH stimulation. Results: We could not demonstrate any consistent differences in serum profiles of known miRNAs between patients with and without recurrent/ persistent disease or before and after rhTSH stimulation. However, our sequencing data revealed 2 putative novel miRNAs that rise with rhTSH stimulation in the serums of patients with recurrent/persistent disease. We further confirmed by qPCR the upregulation of these putative miRNAs both in serums and in TSH-stimulated cells. We also show miRNAs that are good candidates for housekeeping genes in the serum of patients independently of the levels of TSH. Conclusions: The present study does not provide evidence that known miRNAs can be used as circulating markers for recurrence of thyroid cancer. However, we suggest that novel miRNA molecules may be related to thyroid cancer pathogenesis.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10641/3777
url https://hdl.handle.net/10641/3777
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
instname:Universidad Francisco de Vitoria
instname_str Universidad Francisco de Vitoria
reponame_str DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
collection DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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