Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies

This is an open access article distributed under the Creative Commons Attribution License (CC BY).

Detalles Bibliográficos
Autores: Soler, María, Estévez, M. Carmen, Álvarez, Mar, Otte, Marinus A., Sepúlveda, Borja, Lechuga, Laura M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/131510
Acceso en línea:http://hdl.handle.net/10261/131510
Access Level:acceso abierto
Palabra clave:Clinical diagnosis
Gold nanodisks
Nanoplasmonic biosensor
SPR biosensor
Direct immunoassay
ProLinker™
Antibody immobilization
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spelling Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategiesSoler, MaríaEstévez, M. CarmenÁlvarez, MarOtte, Marinus A.Sepúlveda, BorjaLechuga, Laura M.Clinical diagnosisGold nanodisksNanoplasmonic biosensorSPR biosensorDirect immunoassayProLinker™Antibody immobilizationThis is an open access article distributed under the Creative Commons Attribution License (CC BY).This article belongs to the Special Issue Plasmonics and Nanoplasmonics Biosensors.Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis.The work has been done thanks to the MULTIBIOPLAS project (TEC2099-08729) and the INNBIOD project (INNPACTO Subprogram, OPT-2011-1429-010000) from Spanish Ministry of Economy and Competitiveness. M. S. acknowledges financial support from “Programa de Formación de Personal Investigador (FPI)” from the Spanish Ministry of Economy and Competitiveness.We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).Peer ReviewedMultidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas (España)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016201620142016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/131510reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/s140202239Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1315102026-05-22T06:33:51Z
dc.title.none.fl_str_mv Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
title Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
spellingShingle Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
Soler, María
Clinical diagnosis
Gold nanodisks
Nanoplasmonic biosensor
SPR biosensor
Direct immunoassay
ProLinker™
Antibody immobilization
title_short Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
title_full Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
title_fullStr Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
title_full_unstemmed Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
title_sort Direct detection of protein biomarkers in human fluids using site-specific antibody immobilization strategies
dc.creator.none.fl_str_mv Soler, María
Estévez, M. Carmen
Álvarez, Mar
Otte, Marinus A.
Sepúlveda, Borja
Lechuga, Laura M.
author Soler, María
author_facet Soler, María
Estévez, M. Carmen
Álvarez, Mar
Otte, Marinus A.
Sepúlveda, Borja
Lechuga, Laura M.
author_role author
author2 Estévez, M. Carmen
Álvarez, Mar
Otte, Marinus A.
Sepúlveda, Borja
Lechuga, Laura M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas (España)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Clinical diagnosis
Gold nanodisks
Nanoplasmonic biosensor
SPR biosensor
Direct immunoassay
ProLinker™
Antibody immobilization
topic Clinical diagnosis
Gold nanodisks
Nanoplasmonic biosensor
SPR biosensor
Direct immunoassay
ProLinker™
Antibody immobilization
description This is an open access article distributed under the Creative Commons Attribution License (CC BY).
publishDate 2014
dc.date.none.fl_str_mv 2014
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/131510
url http://hdl.handle.net/10261/131510
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/s140202239
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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