Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency

Purpose: Folic acid (FA) is an essential nutrient for normal embryonic development. FA deficiency (FAD) in maternal diet increases the risk of several defects among the progeny, especially, neural tube defects. The eye begins its development from the neural tube; however, the relationship between FA...

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Detalhes bibliográficos
Autores: Sijilmassi, Ouafa, López Alonso, José Manuel, Río Sevilla, Aurora Del, Murillo González, Jorge Alfonso, Barrio Asensio, María Del Carmen
Formato: artículo
Fecha de publicación:2018
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/13141
Acesso em linha:https://hdl.handle.net/20.500.14352/13141
Access Level:acceso abierto
Palavra-chave:577.164.17:611.84
612.015.641.7
519.22-76
617.7
Folic acid deficiency
Ocular biometry
Eye abnormalities
Development
Statistical analysis
Estadística aplicada
Bioquímica (Biología)
Anatomía ocular
Óptica fisiológica
2302 Bioquímica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/13141
network_acronym_str ES
network_name_str España
repository_id_str
spelling Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid DeficiencySijilmassi, OuafaLópez Alonso, José ManuelRío Sevilla, Aurora DelMurillo González, Jorge AlfonsoBarrio Asensio, María Del Carmen577.164.17:611.84612.015.641.7519.22-76617.7Folic acid deficiencyOcular biometryEye abnormalitiesDevelopmentStatistical analysisEstadística aplicadaBioquímica (Biología)Anatomía ocularÓptica fisiológica2302 BioquímicaPurpose: Folic acid (FA) is an essential nutrient for normal embryonic development. FA deficiency (FAD) in maternal diet increases the risk of several defects among the progeny, especially, neural tube defects. The eye begins its development from the neural tube; however, the relationship between FAD and ocular development in the offspring has been little explored and it isn’t known how the FAD affects the formation of the eye. Our objective was to analyze the effect of maternal FAD on mouse embryos ocular biometry. Methods: Female mice C57/BL/6J were distributed into three different groups, according to the assigned diet: control group fed a standard FA diet (2 mg FA/kg), FAD group for short term fed (0 mg FA/kg + 1% succinylsulfathiazole) from the day after mating until day 14.5 of gestation, and FAD group for long term fed the same FA-deficient diet for 6 weeks prior mating and continued with this diet during gestation. A total of 57 embryos (19 embryos of each dietary group) at 14.5 gestational days were evaluated. As indicators of changes in ocular biometry, we analyze two parameters: area and circularity of the lens and whole eye, and the area of the retina. The program used in the treatment and selection of the areas of interest was ImageJ. The statistical analysis was performed by IBM SPSS Statistics 19. Results: Regarding the measures of the area, FA-deficient lenses and eyes were smaller than that of controls. We have also observed increase in the size of the neural retina, spatially, in embryos from females fed FAD diet during long term. On the other hand, as regard to circularity measures, we have seen that eyes and lenses were more circular than control. Conclusion: Maternal FAD diet for a very short term generates morphological changes in ocular structures to the offspring.Taylor & FrancisUniversidad Complutense de Madrid20182018-11-0120182018-11-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/13141reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/131412026-06-02T12:44:21Z
dc.title.none.fl_str_mv Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
title Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
spellingShingle Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
Sijilmassi, Ouafa
577.164.17:611.84
612.015.641.7
519.22-76
617.7
Folic acid deficiency
Ocular biometry
Eye abnormalities
Development
Statistical analysis
Estadística aplicada
Bioquímica (Biología)
Anatomía ocular
Óptica fisiológica
2302 Bioquímica
title_short Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
title_full Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
title_fullStr Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
title_full_unstemmed Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
title_sort Biometric Alterations of Mouse Embryonic Eye Structures Due to Short-Term Folic Acid Deficiency
dc.creator.none.fl_str_mv Sijilmassi, Ouafa
López Alonso, José Manuel
Río Sevilla, Aurora Del
Murillo González, Jorge Alfonso
Barrio Asensio, María Del Carmen
author Sijilmassi, Ouafa
author_facet Sijilmassi, Ouafa
López Alonso, José Manuel
Río Sevilla, Aurora Del
Murillo González, Jorge Alfonso
Barrio Asensio, María Del Carmen
author_role author
author2 López Alonso, José Manuel
Río Sevilla, Aurora Del
Murillo González, Jorge Alfonso
Barrio Asensio, María Del Carmen
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.164.17:611.84
612.015.641.7
519.22-76
617.7
Folic acid deficiency
Ocular biometry
Eye abnormalities
Development
Statistical analysis
Estadística aplicada
Bioquímica (Biología)
Anatomía ocular
Óptica fisiológica
2302 Bioquímica
topic 577.164.17:611.84
612.015.641.7
519.22-76
617.7
Folic acid deficiency
Ocular biometry
Eye abnormalities
Development
Statistical analysis
Estadística aplicada
Bioquímica (Biología)
Anatomía ocular
Óptica fisiológica
2302 Bioquímica
description Purpose: Folic acid (FA) is an essential nutrient for normal embryonic development. FA deficiency (FAD) in maternal diet increases the risk of several defects among the progeny, especially, neural tube defects. The eye begins its development from the neural tube; however, the relationship between FAD and ocular development in the offspring has been little explored and it isn’t known how the FAD affects the formation of the eye. Our objective was to analyze the effect of maternal FAD on mouse embryos ocular biometry. Methods: Female mice C57/BL/6J were distributed into three different groups, according to the assigned diet: control group fed a standard FA diet (2 mg FA/kg), FAD group for short term fed (0 mg FA/kg + 1% succinylsulfathiazole) from the day after mating until day 14.5 of gestation, and FAD group for long term fed the same FA-deficient diet for 6 weeks prior mating and continued with this diet during gestation. A total of 57 embryos (19 embryos of each dietary group) at 14.5 gestational days were evaluated. As indicators of changes in ocular biometry, we analyze two parameters: area and circularity of the lens and whole eye, and the area of the retina. The program used in the treatment and selection of the areas of interest was ImageJ. The statistical analysis was performed by IBM SPSS Statistics 19. Results: Regarding the measures of the area, FA-deficient lenses and eyes were smaller than that of controls. We have also observed increase in the size of the neural retina, spatially, in embryos from females fed FAD diet during long term. On the other hand, as regard to circularity measures, we have seen that eyes and lenses were more circular than control. Conclusion: Maternal FAD diet for a very short term generates morphological changes in ocular structures to the offspring.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-11-01
2018
2018-11-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/13141
url https://hdl.handle.net/20.500.14352/13141
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402998803791872
score 15,300719

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