GuaB3, an overlooked enzyme in cyanobacteria's toolbox that sheds light on IMP dehydrogenase evolution

[EN]IMP dehydrogenase and GMP reductase are enzymes from the same protein family with analogous structures and catalytic mechanisms that have gained attention because of their essential roles in nucleotide metabolism and as potential drug targets. This study focusses on GuaB3, a less-explored enzyme...

Full description

Bibliographic Details
Authors: Hernández-Gómez, Alejandro, Irisarri, Iker, Fernández-Justel, David, Peláez Lamamie de C. Arroyo, Rafael, Jiménez García, Alberto, Revuelta, José Luis, Balsera, Mónica, Martínez Buey, Rubén
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Universidad de Salamanca (USAL)
Repository:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/168980
Online Access:http://hdl.handle.net/10366/168980
Access Level:Open access
Keyword:MP Dehydrogenase
Cyanobacteria
Nucleotide metabolism
Catalysis
Protein evolution
Phylogeny
Nucleotides
IMP Dehydrogenase
IMP deshidrogenasa
nucleótidos
filogenia
catálisis
Description
Summary:[EN]IMP dehydrogenase and GMP reductase are enzymes from the same protein family with analogous structures and catalytic mechanisms that have gained attention because of their essential roles in nucleotide metabolism and as potential drug targets. This study focusses on GuaB3, a less-explored enzyme within this family. Phylogenetic analysis uncovers GuaB3's independent evolution from other members of the family and it predominantly occurs in Cyanobacteria. Within this group, GuaB3 functions as a unique IMP dehydrogenase, while its counterpart in Actinobacteria has a yet unknown function. Synechocystis sp. PCC6803 GuaB3 structures demonstrate differences in the active site compared to canonical IMP dehydrogenases, despite shared catalytic mechanisms. These findings highlight the essential role of GuaB3 in Cyanobacteria, provide insights into the diversity and evolution of the IMP dehydrogenase protein family, and reveal a distinctive characteristic in nucleotide metabolism, potentially aiding in combating harmful cyanobacterial blooms-a growing concern for humans and wildlife.

Similar Items