High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary
Subterranean estuaries are biogeochemically active coastal sites resulting from the underground mixing of fresh aquifer groundwater and seawater. In these systems, microbial activity can largely transform the chemical elements that may reach the sea through submarine groundwater discharge (SGD), but...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/377668 |
| Acceso en línea: | https://hdl.handle.net/2117/377668 https://dx.doi.org/10.1111/mec.16695 |
| Access Level: | acceso abierto |
| Palabra clave: | Groundwater Aquatic prokaryotic communities Coastal aquifer Microbial activity Mixing zone Patescibacteria Seawater intrusion Submarine groundwater discharge Subterranean estuaries Terrestrial–marine interface Aigües subterrànies Àrees temàtiques de la UPC::Enginyeria civil::Geologia::Hidrologia subterrània |
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| dc.title.none.fl_str_mv |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary |
| title |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary |
| spellingShingle |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary Ruiz González, Clara Groundwater Aquatic prokaryotic communities Coastal aquifer Microbial activity Mixing zone Patescibacteria Seawater intrusion Submarine groundwater discharge Subterranean estuaries Terrestrial–marine interface Aigües subterrànies Àrees temàtiques de la UPC::Enginyeria civil::Geologia::Hidrologia subterrània |
| title_short |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary |
| title_full |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary |
| title_fullStr |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary |
| title_full_unstemmed |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary |
| title_sort |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuary |
| dc.creator.none.fl_str_mv |
Ruiz González, Clara Rodríguez Pie, Lara Maister, Olena Rodellas, Valentí Alorda Keinglass, Aaron Diego Feliu, Marc|||0000-0002-4782-3210 Folch Sancho, Albert|||0000-0002-8490-1038 Garcia Orellana, Jordi Gasol, Josep Maria |
| author |
Ruiz González, Clara |
| author_facet |
Ruiz González, Clara Rodríguez Pie, Lara Maister, Olena Rodellas, Valentí Alorda Keinglass, Aaron Diego Feliu, Marc|||0000-0002-4782-3210 Folch Sancho, Albert|||0000-0002-8490-1038 Garcia Orellana, Jordi Gasol, Josep Maria |
| author_role |
author |
| author2 |
Rodríguez Pie, Lara Maister, Olena Rodellas, Valentí Alorda Keinglass, Aaron Diego Feliu, Marc|||0000-0002-4782-3210 Folch Sancho, Albert|||0000-0002-8490-1038 Garcia Orellana, Jordi Gasol, Josep Maria |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Groundwater Aquatic prokaryotic communities Coastal aquifer Microbial activity Mixing zone Patescibacteria Seawater intrusion Submarine groundwater discharge Subterranean estuaries Terrestrial–marine interface Aigües subterrànies Àrees temàtiques de la UPC::Enginyeria civil::Geologia::Hidrologia subterrània |
| topic |
Groundwater Aquatic prokaryotic communities Coastal aquifer Microbial activity Mixing zone Patescibacteria Seawater intrusion Submarine groundwater discharge Subterranean estuaries Terrestrial–marine interface Aigües subterrànies Àrees temàtiques de la UPC::Enginyeria civil::Geologia::Hidrologia subterrània |
| description |
Subterranean estuaries are biogeochemically active coastal sites resulting from the underground mixing of fresh aquifer groundwater and seawater. In these systems, microbial activity can largely transform the chemical elements that may reach the sea through submarine groundwater discharge (SGD), but little is known about the microorganisms thriving in these land-sea transition zones. We present the first spatially-resolved characterization of the bacterial assemblages along a coastal aquifer in the NW Mediterranean, considering the entire subsurface salinity gradient. Combining bulk heterotrophic activity measurements, flow cytometry, microscopy and 16S rRNA gene sequencing we find large variations in prokaryotic abundances, cell size, activity and diversity at both the horizontal and vertical scales that reflect the pronounced physicochemical gradients. The parts of the transect most influenced by freshwater were characterized by smaller cells and lower prokaryotic abundances and heterotrophic production, but some activity hotspots were found at deep low-oxygen saline groundwater sites enriched in nitrite and ammonium. Diverse, heterogeneous and highly endemic communities dominated by Proteobacteria, Patescibacteria, Desulfobacterota and Bacteroidota were observed throughout the aquifer, pointing to clearly differentiated prokaryotic niches across these transition zones and little microbial connectivity between groundwater and Mediterranean seawater habitats. Finally, experimental manipulations unveiled large increases in community heterotrophic activity driven by fast growth of some rare and site-specific groundwater Proteobacteria. Our results indicate that prokaryotic communities within subterranean estuaries are highly heterogeneous in terms of biomass, activity and diversity, suggesting that their role in transforming nutrients will also vary spatially within these terrestrial–marine transition zones. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022-11-01 2022 2022-12-05 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2117/377668 https://dx.doi.org/10.1111/mec.16695 |
| url |
https://hdl.handle.net/2117/377668 https://dx.doi.org/10.1111/mec.16695 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
| dc.source.none.fl_str_mv |
reponame:UPCommons. Portal del coneixement obert de la UPC instname:Universitat Politècnica de Catalunya (UPC) |
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Universitat Politècnica de Catalunya (UPC) |
| reponame_str |
UPCommons. Portal del coneixement obert de la UPC |
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UPCommons. Portal del coneixement obert de la UPC |
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1869402974194761728 |
| spelling |
High spatial heterogeneity and low connectivity of bacterial communities along a Mediterranean subterranean estuaryRuiz González, ClaraRodríguez Pie, LaraMaister, OlenaRodellas, ValentíAlorda Keinglass, AaronDiego Feliu, Marc|||0000-0002-4782-3210Folch Sancho, Albert|||0000-0002-8490-1038Garcia Orellana, JordiGasol, Josep MariaGroundwaterAquatic prokaryotic communitiesCoastal aquiferMicrobial activityMixing zonePatescibacteriaSeawater intrusionSubmarine groundwater dischargeSubterranean estuariesTerrestrial–marine interfaceAigües subterràniesÀrees temàtiques de la UPC::Enginyeria civil::Geologia::Hidrologia subterràniaSubterranean estuaries are biogeochemically active coastal sites resulting from the underground mixing of fresh aquifer groundwater and seawater. In these systems, microbial activity can largely transform the chemical elements that may reach the sea through submarine groundwater discharge (SGD), but little is known about the microorganisms thriving in these land-sea transition zones. We present the first spatially-resolved characterization of the bacterial assemblages along a coastal aquifer in the NW Mediterranean, considering the entire subsurface salinity gradient. Combining bulk heterotrophic activity measurements, flow cytometry, microscopy and 16S rRNA gene sequencing we find large variations in prokaryotic abundances, cell size, activity and diversity at both the horizontal and vertical scales that reflect the pronounced physicochemical gradients. The parts of the transect most influenced by freshwater were characterized by smaller cells and lower prokaryotic abundances and heterotrophic production, but some activity hotspots were found at deep low-oxygen saline groundwater sites enriched in nitrite and ammonium. Diverse, heterogeneous and highly endemic communities dominated by Proteobacteria, Patescibacteria, Desulfobacterota and Bacteroidota were observed throughout the aquifer, pointing to clearly differentiated prokaryotic niches across these transition zones and little microbial connectivity between groundwater and Mediterranean seawater habitats. Finally, experimental manipulations unveiled large increases in community heterotrophic activity driven by fast growth of some rare and site-specific groundwater Proteobacteria. Our results indicate that prokaryotic communities within subterranean estuaries are highly heterogeneous in terms of biomass, activity and diversity, suggesting that their role in transforming nutrients will also vary spatially within these terrestrial–marine transition zones.This work was funded by the Spanish Ministry of Science, Innovation and Universities (MICINN) through the GRAMMI project (RTI2018-099740-J-I00), the Ramon y Cajal contract (RYC2019-026758-I) and partly by the project MEDISTRAES III (PID2019-110311RB-C22 and PID2019-110212RB-C21). Additional financial support was provided by grants SPIP2020-02595 (Ministry for the Ecological Transition and the Demographic Challenge [MITECO]) with funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S). JGO acknowledges the financial support of the Spanish Ministry of Science, Innovation and Universities, through the “Maria de Maeztu” programme for Units of Excellence (CEX2019-000940-M). VR acknowledges financial support from the Beatriu de Pinós postdoctoral programme of the Catalan Government (2019-BP-00241). AAK acknowledges financial support from ICTA “Unit of Excellence” (MinECo, MDM2015-0552-17-1) and PhD fellowship, BES-2017-080740. The coauthor AF is a Serra Húnter Fellow. MDF acknowledges the economic support from the FI-2017 fellowships of the Generalitat de Catalunya autonomous government (2017FI_B_00365). The authors would like to thank the support of the Generalitat de Catalunya to MERS (2017 SGR-1588), GHS (2017 SGR 1485) and 2017SGR/156, for additional funding. We would like to thank SIMMAR (Serveis Integrals de Manteniment del Maresme) and the Consell Comarcal del Maresme in the construction of the research site. A special word for Jordi Garcia-Orellana, great mentor and friend, who left us recently and unexpectedly, and without whom this and much of the ongoing work would not exist. May he rest in peace.Peer Reviewed20222022-11-0120222022-12-05journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/377668https://dx.doi.org/10.1111/mec.16695reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3776682026-05-27T15:37:01Z |
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15,301603 |