Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols

In this study, we have evaluated by HPLC-DAD, DLS and MALDI-TOF a synergic effect of the coexistence of two salivary-PRP fractions (basic-PRPs and acidic PRPs) on the interaction with flavanols. Results obtained showed noticeable enhancement of the interaction between (epi)catechin and PRPs when bot...

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Detalles Bibliográficos
Autores: Ramos-Pineda, A.M., García-Estévez, I., Soares, S., de Freitas, V., Dueñas, M., Escribano Bailón, María Teresa
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/161882
Acceso en línea:http://hdl.handle.net/10366/161882
Access Level:acceso abierto
Palabra clave:Salivary proline-rich proteins
Interaction
Flavan-3-ols
Astringency
HPLC-DAD
MALDI-TOF
Dynamic-light-scattering
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spelling Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanolsRamos-Pineda, A.M.García-Estévez, I.Soares, S.de Freitas, V.Dueñas, M.Escribano Bailón, María TeresaSalivary proline-rich proteinsInteractionFlavan-3-olsAstringencyHPLC-DADMALDI-TOFDynamic-light-scatteringIn this study, we have evaluated by HPLC-DAD, DLS and MALDI-TOF a synergic effect of the coexistence of two salivary-PRP fractions (basic-PRPs and acidic PRPs) on the interaction with flavanols. Results obtained showed noticeable enhancement of the interaction between (epi)catechin and PRPs when both types of proteins are blended. Up to 30 soluble aggregates have been tentatively identified with molecular weight from 4680 to 35,851. (epi)Catechins seem to bind preferentially bPRPs than aPRPs, although the medium size aggregates flavanol-bPRPs formed could favour the interaction with aPRPs giving rise to soluble mixed aggregates.Elsevier202520252019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/161882reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAGL2017-84793-C2-1-R0377_IBERPHENOL_6_EAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1618822026-06-07T06:28:51Z
dc.title.none.fl_str_mv Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols
title Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols
spellingShingle Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols
Ramos-Pineda, A.M.
Salivary proline-rich proteins
Interaction
Flavan-3-ols
Astringency
HPLC-DAD
MALDI-TOF
Dynamic-light-scattering
title_short Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols
title_full Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols
title_fullStr Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols
title_full_unstemmed Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols
title_sort Synergistic effect of mixture of two proline-rich-protein salivary families (aPRP and bPRP) on the interaction with wine flavanols
dc.creator.none.fl_str_mv Ramos-Pineda, A.M.
García-Estévez, I.
Soares, S.
de Freitas, V.
Dueñas, M.
Escribano Bailón, María Teresa
author Ramos-Pineda, A.M.
author_facet Ramos-Pineda, A.M.
García-Estévez, I.
Soares, S.
de Freitas, V.
Dueñas, M.
Escribano Bailón, María Teresa
author_role author
author2 García-Estévez, I.
Soares, S.
de Freitas, V.
Dueñas, M.
Escribano Bailón, María Teresa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Salivary proline-rich proteins
Interaction
Flavan-3-ols
Astringency
HPLC-DAD
MALDI-TOF
Dynamic-light-scattering
topic Salivary proline-rich proteins
Interaction
Flavan-3-ols
Astringency
HPLC-DAD
MALDI-TOF
Dynamic-light-scattering
description In this study, we have evaluated by HPLC-DAD, DLS and MALDI-TOF a synergic effect of the coexistence of two salivary-PRP fractions (basic-PRPs and acidic PRPs) on the interaction with flavanols. Results obtained showed noticeable enhancement of the interaction between (epi)catechin and PRPs when both types of proteins are blended. Up to 30 soluble aggregates have been tentatively identified with molecular weight from 4680 to 35,851. (epi)Catechins seem to bind preferentially bPRPs than aPRPs, although the medium size aggregates flavanol-bPRPs formed could favour the interaction with aPRPs giving rise to soluble mixed aggregates.
publishDate 2019
dc.date.none.fl_str_mv 2019
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/161882
url http://hdl.handle.net/10366/161882
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv AGL2017-84793-C2-1-R
0377_IBERPHENOL_6_E
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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