Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats

10 páginas, 4 tablas, 1 figura.

Detalles Bibliográficos
Autores: Ramos Morales, Eva, Arco Pérez, Ana, Martín García, A. Ignacio, Yáñez Ruiz, David R., Frutos, Pilar, Hervás, Gonzalo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/107709
Acceso en línea:http://hdl.handle.net/10261/107709
Access Level:acceso abierto
Palabra clave:Fermentation
Microorganisms
Rumen cannula
Small ruminants
Stomach tube
id ES_0745dbae14aa2a5c73c5f17e95bddade
oai_identifier_str oai:digital.csic.es:10261/107709
network_acronym_str ES
network_name_str España
repository_id_str
spelling Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goatsRamos Morales, EvaArco Pérez, AnaMartín García, A. IgnacioYáñez Ruiz, David R.Frutos, PilarHervás, GonzaloFermentationMicroorganismsRumen cannulaSmall ruminantsStomach tube10 páginas, 4 tablas, 1 figura.Rumen cannulation is the reference method for collection of representative samples of rumen digesta. However, it is not always viable, which obliges to depend on less invasive techniques, such as stomach tubing. The aim of this work was to study if the differences in fermentation parameters and rumen microbial populations observed between species (sheep and goats), diets (forage and forage plus concentrate) and sampling times (pre- and post-feeding) are consistent when collecting the samples through stomach tube or rumen cannula, in an attempt to validate the use of the former as an alternative to the latter. Four sheep and four goats, fitted with ruminal cannula, were fed either forage (F diet; alfalfa hay) or forage plus concentrate (1:1; FC diet), in two 15-day periods. At the end of each period (days 14 and 15), samples of rumen digesta were taken by stomach tube and rumen cannula, before and 4. h after morning feeding, for determination of ruminal fermentation parameters (pH, and lactate, ammonia and total VFA concentrations). The three main rumen microbial groups (bacteria, protozoa and methanogenic archaea) and two fibrolytic bacteria ( Ruminococcus flavefaciens and Fibrobacter succinogenes) were quantified by real-time PCR and, additionally, PCR-DGGE analysis of the bacterial community on the rumen digesta samples collected post-feeding was carried out. Overall, sampling through ruminal cannula and stomach tube gave similar results regarding fermentation parameters when comparing species, diets and sampling times. Despite samples for microbiology assays contained liquid plus solid fractions when collected through rumen cannula and mostly liquid when collected through stomach tube, both techniques showed certain consistency in the effects of treatments on the rumen microbiota ( e.g., both revealed no differences between species in total bacteria, archaea and R. flavefaciens concentrations, and higher protozoa numbers in goats than in sheep). However, there was also some discrepancy regarding microorganism concentrations, particularly concerning sampling times ( e.g., differences between pre- and post-feeding samplings were only observed in rumen cannula samples for total bacteria and methanogenic archaea, and in stomach tube samples for R. flavefaciens concentrations). Therefore, this study supports that non-invasive stomach tubing is a feasible alternative to surgical rumen cannulation in sheep and goats to examine ruminal fermentation. Nonetheless, caution should be taken when using this technique to assess the structure and composition of the rumen microbial community.This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO; AGL2011-27218 and AGL2011-23700). E. Ramos-Morales acknowledge the receipt of a research contract from the Spanish National Research Council (CSIC), Junta de Ampliación de Estudios (JAEdoc) Programme.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/107709reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.anifeedsci.2014.09.016Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1077092026-05-22T06:33:51Z
dc.title.none.fl_str_mv Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats
title Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats
spellingShingle Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats
Ramos Morales, Eva
Fermentation
Microorganisms
Rumen cannula
Small ruminants
Stomach tube
title_short Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats
title_full Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats
title_fullStr Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats
title_full_unstemmed Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats
title_sort Use of stomach tubing as an alternative to rumen cannulation to study ruminal fermentation and microbiota in sheep and goats
dc.creator.none.fl_str_mv Ramos Morales, Eva
Arco Pérez, Ana
Martín García, A. Ignacio
Yáñez Ruiz, David R.
Frutos, Pilar
Hervás, Gonzalo
author Ramos Morales, Eva
author_facet Ramos Morales, Eva
Arco Pérez, Ana
Martín García, A. Ignacio
Yáñez Ruiz, David R.
Frutos, Pilar
Hervás, Gonzalo
author_role author
author2 Arco Pérez, Ana
Martín García, A. Ignacio
Yáñez Ruiz, David R.
Frutos, Pilar
Hervás, Gonzalo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fermentation
Microorganisms
Rumen cannula
Small ruminants
Stomach tube
topic Fermentation
Microorganisms
Rumen cannula
Small ruminants
Stomach tube
description 10 páginas, 4 tablas, 1 figura.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/107709
url http://hdl.handle.net/10261/107709
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.anifeedsci.2014.09.016
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402972681666560
score 15.812429

Similares en LA Referencia