Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection

Producción Científica

Detalhes bibliográficos
Autores: Ares Sacristán, Ana María, Martín Gómez, María Teresa, Toribio Recio, Laura, Bernal del Nozal, José
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/53850
Acesso em linha:https://doi.org/10.1007/s12161-022-02281-8
https://uvadoc.uva.es/handle/10324/53850
Access Level:acceso abierto
Palavra-chave:Bee pollen
Derivatization
Fluorescence detection
Free amino acid
Liquid chromatography
Solvent extraction
23 Química
2301 Química Analítica
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oai_identifier_str oai:uvadoc.uva.es:10324/53850
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repository_id_str
spelling Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detectionAres Sacristán, Ana MaríaMartín Gómez, María TeresaToribio Recio, LauraBernal del Nozal, JoséBee pollenDerivatizationFluorescence detectionFree amino acidLiquid chromatographySolvent extraction23 Química2301 Química AnalíticaProducción CientíficaBee pollen is one of the hive products that is of most interest today due to its multiple beneficial health properties, making it an increasingly popular food supplement. Bee pollen contains many bioactive compounds, such as fatty acids, vitamins, minerals, proteins, and amino acids, among others. In the present study, the free amino acid content was determined in bee pollen by using liquid chromatography coupled to a fluorescence detector. Sample treatment consisted of a solvent extraction of the free amino acids with ultrapure water and a further centrifugation of the extract, which was repeated twice. After that, it was necessary to perform a pre-column derivatization of the amino acids using a combination of two reagents (o-phthalaldehyde and 9-fluorenylmethyl chloroformate) prior to their separation in a Gemini® C18 reverse phase column in gradient elution mode. The analytical performance was evaluated, and several commercial bee pollen samples were analyzed. Significant differences in the free amino acid profile and concentration, which ranged between 19 and 192 mg/g, were observed depending on the botanical origin of the samples.Ministerio de Economía y Competitividad y el Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (project number RTA 2015–00013-C03-03)Publicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCLESpringer2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1007/s12161-022-02281-8https://uvadoc.uva.es/handle/10324/53850reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://link.springer.com/article/10.1007/s12161-022-02281-8info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:uvadoc.uva.es:10324/538502026-06-13T12:44:47Z
dc.title.none.fl_str_mv Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
title Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
spellingShingle Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
Ares Sacristán, Ana María
Bee pollen
Derivatization
Fluorescence detection
Free amino acid
Liquid chromatography
Solvent extraction
23 Química
2301 Química Analítica
title_short Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
title_full Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
title_fullStr Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
title_full_unstemmed Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
title_sort Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
dc.creator.none.fl_str_mv Ares Sacristán, Ana María
Martín Gómez, María Teresa
Toribio Recio, Laura
Bernal del Nozal, José
author Ares Sacristán, Ana María
author_facet Ares Sacristán, Ana María
Martín Gómez, María Teresa
Toribio Recio, Laura
Bernal del Nozal, José
author_role author
author2 Martín Gómez, María Teresa
Toribio Recio, Laura
Bernal del Nozal, José
author2_role author
author
author
dc.subject.none.fl_str_mv Bee pollen
Derivatization
Fluorescence detection
Free amino acid
Liquid chromatography
Solvent extraction
23 Química
2301 Química Analítica
topic Bee pollen
Derivatization
Fluorescence detection
Free amino acid
Liquid chromatography
Solvent extraction
23 Química
2301 Química Analítica
description Producción Científica
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1007/s12161-022-02281-8
https://uvadoc.uva.es/handle/10324/53850
url https://doi.org/10.1007/s12161-022-02281-8
https://uvadoc.uva.es/handle/10324/53850
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://link.springer.com/article/10.1007/s12161-022-02281-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402971980169216
score 15,300719

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