Efectos de parámetros ambientales en interacciones compatibles entre plantas y virus de RNA y su relación con los supresores de silenciamiento virales y sus propiedades
[EN] This Research Report presents and discusses results obtained in work that on the one hand studies effects that the environmental factors temperature and ambient CO2 levels cause on compatible infections of plants by positive-sense RNA viruses and also on the antiviral silencing defense and its...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/150806 |
| Acceso en línea: | http://hdl.handle.net/10261/150806 |
| Access Level: | acceso abierto |
| Palabra clave: | Plant-virus interaction Climate change Elevated temperature Elevated CO2 levels Potato virus Y, PVY Potato virus X, PVX PVX vectors HCPro suppression of silencing |
| Sumario: | [EN] This Research Report presents and discusses results obtained in work that on the one hand studies effects that the environmental factors temperature and ambient CO2 levels cause on compatible infections of plants by positive-sense RNA viruses and also on the antiviral silencing defense and its suppression by viral factors. And on the other hand, studies the properties of the viral suppressor of silencing HCPro from Potato virus Y (PVY), using cell and molecular biology experimental approaches. To study how increases in the values of those two environmental factors could affect viral infections of plants we addressed each separately in infections of the host Nicotiana benthamiana by several viruses: Cucumber mosaic virus (CMV), PVY, and a viral expression vector derived from Potato virus X (PVX) either empty, or expressing the suppressors of silencing of PVY or CMV, HCPro and 2b protein, respectively. Systemic viral accumulation levels, infection symptoms, and the activities of the viral suppressors were analyzed under elevated temperature conditions (30 ºC) or under elevated ambient levels of CO2 (970 parts per million, ppm) and were compared to those found at the “normal” conditions of 25 ºC and 401 ppm levels of CO2. |
|---|