Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour

Extraction solvent is a very important factor in the recovery of antioxidants from natural matrices. In this study, the effect of three solvents (ethanol, ethanol/water and water) on the phenolic composition, antioxidant and anti-cholinesterase activities and electrochemical behaviour of four winema...

Descripción completa

Detalles Bibliográficos
Autores: Jara Palacios, Mª José, Gonçalves, Sandra, Heredia Mira, Francisco José, Hernanz Vila, María Dolores, Romano, Anabela
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/100996
Acceso en línea:https://hdl.handle.net/11441/100996
https://doi.org/10.3390/antiox9080675
Access Level:acceso abierto
Palabra clave:Grape pomace
UHPLC
DPPH
ORAC
Acetylcholinesterase
Butyrylcholinesterase
Electrochemistry
id ES_06dfa05c3f169720db30fa69aead29a2
oai_identifier_str oai:idus.us.es:11441/100996
network_acronym_str ES
network_name_str España
repository_id_str
spelling Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical BehaviourJara Palacios, Mª JoséGonçalves, SandraHeredia Mira, Francisco JoséHernanz Vila, María DoloresRomano, AnabelaGrape pomaceUHPLCDPPHORACAcetylcholinesteraseButyrylcholinesteraseElectrochemistryExtraction solvent is a very important factor in the recovery of antioxidants from natural matrices. In this study, the effect of three solvents (ethanol, ethanol/water and water) on the phenolic composition, antioxidant and anti-cholinesterase activities and electrochemical behaviour of four winemaking byproducts (seeds, skins, stems, and pomace) was evaluated. Phenolic composition was determined by the Folin–Ciocalteu method and ultra-high-performance liquid chromatography (UHPLC), antioxidant activity by the capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, anti-cholinesterase activity by the Ellman’s method, and electrochemical behaviour by cyclic voltammetry. Eight phenolic compounds were quantified with higher content in water/ethanol extracts (e.g., epicatechin in pomace: 17 mg/100 g vs. 7 and 6 mg/100 g in ethanol and water extracts, respectively), although there were some exceptions (e.g., gallic acid in seeds was most abundant in water extracts). Moreover, the highest total phenolic content (TPC) and antioxidant activity were found in ethanol/water extracts (between 2 and 30-fold the values of the other extracts). Overall, the most active extracts in inhibiting both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were ethanol/water and ethanol extracts from seeds (between 31.11 and 53.90%). The electrochemical behaviour allowed for differentiating the extracts depending on the solvent and the byproduct. Our findings indicate that winemaking byproducts represent a source of phenolic compounds with antioxidant and anti-cholinesterase activities and suggest that cyclic voltammetry is a promising technique to evaluate the phenolic extraction process from these byproducts.Ministerio de Economía y Competitividad AGL2017-84793-C2-2-RMultidisciplinary Digital Publishing Institute (MDPI)Química AnalíticaNutrición y Bromatología, Toxicología y Medicina LegalUniversidad de Sevilla PP2020-143262020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/100996https://doi.org/10.3390/antiox9080675reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésAntioxidants, 9 (8), 675-.AGL2017-84793-C2-2-RPP2020-14326https://doi.org/10.3390/antiox9080675info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1009962026-06-17T12:51:07Z
dc.title.none.fl_str_mv Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour
title Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour
spellingShingle Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour
Jara Palacios, Mª José
Grape pomace
UHPLC
DPPH
ORAC
Acetylcholinesterase
Butyrylcholinesterase
Electrochemistry
title_short Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour
title_full Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour
title_fullStr Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour
title_full_unstemmed Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour
title_sort Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour
dc.creator.none.fl_str_mv Jara Palacios, Mª José
Gonçalves, Sandra
Heredia Mira, Francisco José
Hernanz Vila, María Dolores
Romano, Anabela
author Jara Palacios, Mª José
author_facet Jara Palacios, Mª José
Gonçalves, Sandra
Heredia Mira, Francisco José
Hernanz Vila, María Dolores
Romano, Anabela
author_role author
author2 Gonçalves, Sandra
Heredia Mira, Francisco José
Hernanz Vila, María Dolores
Romano, Anabela
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Química Analítica
Nutrición y Bromatología, Toxicología y Medicina Legal
Universidad de Sevilla PP2020-14326
dc.subject.none.fl_str_mv Grape pomace
UHPLC
DPPH
ORAC
Acetylcholinesterase
Butyrylcholinesterase
Electrochemistry
topic Grape pomace
UHPLC
DPPH
ORAC
Acetylcholinesterase
Butyrylcholinesterase
Electrochemistry
description Extraction solvent is a very important factor in the recovery of antioxidants from natural matrices. In this study, the effect of three solvents (ethanol, ethanol/water and water) on the phenolic composition, antioxidant and anti-cholinesterase activities and electrochemical behaviour of four winemaking byproducts (seeds, skins, stems, and pomace) was evaluated. Phenolic composition was determined by the Folin–Ciocalteu method and ultra-high-performance liquid chromatography (UHPLC), antioxidant activity by the capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, anti-cholinesterase activity by the Ellman’s method, and electrochemical behaviour by cyclic voltammetry. Eight phenolic compounds were quantified with higher content in water/ethanol extracts (e.g., epicatechin in pomace: 17 mg/100 g vs. 7 and 6 mg/100 g in ethanol and water extracts, respectively), although there were some exceptions (e.g., gallic acid in seeds was most abundant in water extracts). Moreover, the highest total phenolic content (TPC) and antioxidant activity were found in ethanol/water extracts (between 2 and 30-fold the values of the other extracts). Overall, the most active extracts in inhibiting both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were ethanol/water and ethanol extracts from seeds (between 31.11 and 53.90%). The electrochemical behaviour allowed for differentiating the extracts depending on the solvent and the byproduct. Our findings indicate that winemaking byproducts represent a source of phenolic compounds with antioxidant and anti-cholinesterase activities and suggest that cyclic voltammetry is a promising technique to evaluate the phenolic extraction process from these byproducts.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/100996
https://doi.org/10.3390/antiox9080675
url https://hdl.handle.net/11441/100996
https://doi.org/10.3390/antiox9080675
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Antioxidants, 9 (8), 675-.
AGL2017-84793-C2-2-R
PP2020-14326
https://doi.org/10.3390/antiox9080675
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402944632258560
score 15.300719

Similares en LA Referencia