The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific

Gene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances, this crosstalk contributes to buffer mRNA at a roughl...

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Detalles Bibliográficos
Autores: García Martínez, José, Medina, Daniel A., Bellvís, P., Sun, M., Cramer, P., Chávez de Diego, Sebastián, Pérez Ortín, José E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/144940
Acceso en línea:https://hdl.handle.net/11441/144940
https://doi.org/10.1261/rna. 078774.121
Access Level:acceso abierto
Palabra clave:Aneuploidy
Crosstalk
MRNA decay
NMD
Transcription
Yeast
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spelling The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specificGarcía Martínez, JoséMedina, Daniel A.Bellvís, P.Sun, M.Cramer, P.Chávez de Diego, SebastiánPérez Ortín, José E.AneuploidyCrosstalkMRNA decayNMDTranscriptionYeastGene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances, this crosstalk contributes to buffer mRNA at a roughly constant concentration. Whether the mRNA buffering concept operates on the total mRNA concentration or at the gene-specific level, and if the mechanism to do so is a global or a specific one, remain unknown. Here we assessed changes in mRNA concentrations and their synthesis rates along the transcriptome of aneuploid strains of the yeast Saccharomyces cerevisiae. We also assessed mRNA concentrations and their synthesis rates in nonsense-mediated decay (NMD) targets in euploid strains. We found that the altered synthesis rates in the genes from the aneuploid chromosome and the changes in their mRNA stabilities were not counterbalanced. In addition, the stability of NMD targets was not specifically compensated by the changes in synthesis rate. We conclude that there is no genetic compensation of NMD mRNA targets in yeast, and total mRNA buffering uses mostly a global system rather than a gene-specific one.European Commission. Fondo Europeo de Desarrollo Regional BFU2016-77728-C3-1-P, BFU2016-77728-C3-3-P, PID2020-112853GB-C31, RED2018-102467-TJunta de Andalucía BIO271Generalitat Valenciana AICO2019/088Cold Spring Harbor Laboratory PressGenéticaEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)Junta de AndalucíaGeneralitat Valenciana. España2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/144940https://doi.org/10.1261/rna. 078774.121reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésRNA, 27 (10), 1281-1290.BFU2016-77728-C3-1-PBFU2016-77728-C3-3-PPID2020-112853GB-C31RED2018-102467-TBIO271AICO2019/088https://dx.doi.org/10.1261/rna.078774.121info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1449402026-06-17T12:51:07Z
dc.title.none.fl_str_mv The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific
title The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific
spellingShingle The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific
García Martínez, José
Aneuploidy
Crosstalk
MRNA decay
NMD
Transcription
Yeast
title_short The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific
title_full The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific
title_fullStr The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific
title_full_unstemmed The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific
title_sort The Total mRNA Concentration Buffering System in Yeast is Global Rather than Gene-specific
dc.creator.none.fl_str_mv García Martínez, José
Medina, Daniel A.
Bellvís, P.
Sun, M.
Cramer, P.
Chávez de Diego, Sebastián
Pérez Ortín, José E.
author García Martínez, José
author_facet García Martínez, José
Medina, Daniel A.
Bellvís, P.
Sun, M.
Cramer, P.
Chávez de Diego, Sebastián
Pérez Ortín, José E.
author_role author
author2 Medina, Daniel A.
Bellvís, P.
Sun, M.
Cramer, P.
Chávez de Diego, Sebastián
Pérez Ortín, José E.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Junta de Andalucía
Generalitat Valenciana. España
dc.subject.none.fl_str_mv Aneuploidy
Crosstalk
MRNA decay
NMD
Transcription
Yeast
topic Aneuploidy
Crosstalk
MRNA decay
NMD
Transcription
Yeast
description Gene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances, this crosstalk contributes to buffer mRNA at a roughly constant concentration. Whether the mRNA buffering concept operates on the total mRNA concentration or at the gene-specific level, and if the mechanism to do so is a global or a specific one, remain unknown. Here we assessed changes in mRNA concentrations and their synthesis rates along the transcriptome of aneuploid strains of the yeast Saccharomyces cerevisiae. We also assessed mRNA concentrations and their synthesis rates in nonsense-mediated decay (NMD) targets in euploid strains. We found that the altered synthesis rates in the genes from the aneuploid chromosome and the changes in their mRNA stabilities were not counterbalanced. In addition, the stability of NMD targets was not specifically compensated by the changes in synthesis rate. We conclude that there is no genetic compensation of NMD mRNA targets in yeast, and total mRNA buffering uses mostly a global system rather than a gene-specific one.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/144940
https://doi.org/10.1261/rna. 078774.121
url https://hdl.handle.net/11441/144940
https://doi.org/10.1261/rna. 078774.121
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv RNA, 27 (10), 1281-1290.
BFU2016-77728-C3-1-P
BFU2016-77728-C3-3-P
PID2020-112853GB-C31
RED2018-102467-T
BIO271
AICO2019/088
https://dx.doi.org/10.1261/rna.078774.121
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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