Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor

An amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized...

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Autores: Pinacho, Daniel G., Sánchez Baeza, Francisco, Pividori, María Isabel|||0000-0002-5266-7873, Marco, María Pilar|||0000-0002-4064-1668
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:146729
Acceso en línea:https://ddd.uab.cat/record/146729
https://dx.doi.org/urn:doi:10.3390/s140915965
Access Level:acceso abierto
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spelling Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto ImmunosensorPinacho, Daniel G.Sánchez Baeza, FranciscoPividori, María Isabel|||0000-0002-5266-7873Marco, María Pilar|||0000-0002-4064-1668An amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized enzyme and a magnetic graphite-epoxy composite (m-GEC) electrode. After the immunochemical reaction with specific enzyme tracer, the antibody biomodified magnetic beads are easily captured by an electrode made of graphite-epoxy composite containing a magnet, which also acts as transducer for the electrochemical detection. In spite of the complexity of milk, the use of magnetic beads allows elimination of potential interferences caused by the matrix components; hence the AMIS could perform quantitative measurements, directly in these samples, without any additional sample cleanup or extraction step. The immunosensor is able to detect up to seven different fluoroquinolones far below the MRLs defined by the UE for milk; for example ciprofloxacin is detected directly in milk with an IC50 of 0.74 μg/L and a LOD of 0.009 μg/L. This strategy offers great promise for rapid, simple, cost-effective, and on-site analysis fluoroquinolones in complex samples. 22014-01-0120142014-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/146729https://dx.doi.org/urn:doi:10.3390/s140915965reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1467292026-06-06T12:50:31Z
dc.title.none.fl_str_mv Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor
title Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor
spellingShingle Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor
Pinacho, Daniel G.
title_short Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor
title_full Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor
title_fullStr Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor
title_full_unstemmed Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor
title_sort Electrochemical Detection of Fluoroquinolone Antibiotics in Milk Using a Magneto Immunosensor
dc.creator.none.fl_str_mv Pinacho, Daniel G.
Sánchez Baeza, Francisco
Pividori, María Isabel|||0000-0002-5266-7873
Marco, María Pilar|||0000-0002-4064-1668
author Pinacho, Daniel G.
author_facet Pinacho, Daniel G.
Sánchez Baeza, Francisco
Pividori, María Isabel|||0000-0002-5266-7873
Marco, María Pilar|||0000-0002-4064-1668
author_role author
author2 Sánchez Baeza, Francisco
Pividori, María Isabel|||0000-0002-5266-7873
Marco, María Pilar|||0000-0002-4064-1668
author2_role author
author
author
description An amperometric magneto-immunosensor (AMIS) for the detection of residues of fluoroquinolone antibiotics in milk samples is described for the first time. The immunosensor presented combines magnetic beads biomodified with an antibody with a broad recognition profile of fluoroquinolones, a haptenized enzyme and a magnetic graphite-epoxy composite (m-GEC) electrode. After the immunochemical reaction with specific enzyme tracer, the antibody biomodified magnetic beads are easily captured by an electrode made of graphite-epoxy composite containing a magnet, which also acts as transducer for the electrochemical detection. In spite of the complexity of milk, the use of magnetic beads allows elimination of potential interferences caused by the matrix components; hence the AMIS could perform quantitative measurements, directly in these samples, without any additional sample cleanup or extraction step. The immunosensor is able to detect up to seven different fluoroquinolones far below the MRLs defined by the UE for milk; for example ciprofloxacin is detected directly in milk with an IC50 of 0.74 μg/L and a LOD of 0.009 μg/L. This strategy offers great promise for rapid, simple, cost-effective, and on-site analysis fluoroquinolones in complex samples.
publishDate 2014
dc.date.none.fl_str_mv 2
2014-01-01
2014
2014-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/146729
https://dx.doi.org/urn:doi:10.3390/s140915965
url https://ddd.uab.cat/record/146729
https://dx.doi.org/urn:doi:10.3390/s140915965
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/3.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/3.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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