Storage conditions and stability of global DNA methylation in placental tissue
AIM: The placenta is an informative and easily available tissue for many epidemiological studies. We analyzed the extent to which storage delay affects DNA methylation. MATERIAL & METHODS: Biopsies from two placentas were sequentially stored at -80°C after standing at room temperature for 30...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/36592 |
| Acceso en línea: | http://hdl.handle.net/10230/36592 http://dx.doi.org/10.2217/epi.13.29 |
| Access Level: | acceso abierto |
| Palabra clave: | ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme |
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Storage conditions and stability of global DNA methylation in placental tissueVilahur Chiaraviglio, Nadia, 1982-Baccarelli, Andrea A.Bustamante Pineda, MarionaAgramunt, SilviaByun, Hyang-MinFernandez, Mariana F.Sunyer Deu, JordiEstivill, Xavier, 1955-ADN -- MetabolismeADN -- MetilacióPlacenta -- MetabolismeAIM: The placenta is an informative and easily available tissue for many epidemiological studies. We analyzed the extent to which storage delay affects DNA methylation. MATERIAL & METHODS: Biopsies from two placentas were sequentially stored at -80°C after standing at room temperature for 30 min, 1 h, 2 h, 6 h and 24 h. Global DNA methylation was measured by bisulfite pyrosequencing of repetitive elements and the luminometric methylation assay. RESULTS: Small changes in global DNA methylation in relation to time-to-storage were observed by pyrosequencing, with a coefficient of variation (COV) of 2.49% (placenta 1) and 2.86% (placenta 2), similar to the mean technical variation observed for pyrosequencing (COV: 1.91 and 1.51%, respectively). A luminometric methylation assay yielded more variable results in the two placentas analyzed, both among time points (COV: 9.13 and 10.35%, respectively) and technical replicates (COV: 11.60 and 9.80%, respectively). CONCLUSION: Global DNA methylation is stable at room temperature. However, some techniques to measure methylation might be confounded by DNA degradation caused by a delay in storage.This study was funded by grants from the Spanish Ministry of Health (FIS-PI041436 and PI11/00610), Instituto de Salud Carlos III (Red Infancia y MedioAmbiente G03/176 and CB06/02/0041) and the Generalitat de Catalunya-Comissió Interdepartamental de Recerca i Innovació Tecnològica 1999SGR 00241. AA Baccarelli receives support from the Harvard School of Public Health and National Institute of Environmental Health Sciences Center for Environmental Health (ES000002). N Vilahur was supported by a Formación de Personal Investigador Grant from the Spanish Ministry of Health and a Formación de Personal Investigador Grant for Short Research Stays in Foreign Institutions (BES-2009-023933) and MF Fernandez by the Ramon y Cajal Research Grant from the Spanish Ministry of EducationFuture Medicine201920192013info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/36592http://dx.doi.org/10.2217/epi.13.29reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésEpigenomics. 2013 Jun;5(3):341-8© 2013 Future Medicine Ltdinfo:eu-repo/semantics/openAccessoai:recercat.cat:10230/365922026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Storage conditions and stability of global DNA methylation in placental tissue |
| title |
Storage conditions and stability of global DNA methylation in placental tissue |
| spellingShingle |
Storage conditions and stability of global DNA methylation in placental tissue Vilahur Chiaraviglio, Nadia, 1982- ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme |
| title_short |
Storage conditions and stability of global DNA methylation in placental tissue |
| title_full |
Storage conditions and stability of global DNA methylation in placental tissue |
| title_fullStr |
Storage conditions and stability of global DNA methylation in placental tissue |
| title_full_unstemmed |
Storage conditions and stability of global DNA methylation in placental tissue |
| title_sort |
Storage conditions and stability of global DNA methylation in placental tissue |
| dc.creator.none.fl_str_mv |
Vilahur Chiaraviglio, Nadia, 1982- Baccarelli, Andrea A. Bustamante Pineda, Mariona Agramunt, Silvia Byun, Hyang-Min Fernandez, Mariana F. Sunyer Deu, Jordi Estivill, Xavier, 1955- |
| author |
Vilahur Chiaraviglio, Nadia, 1982- |
| author_facet |
Vilahur Chiaraviglio, Nadia, 1982- Baccarelli, Andrea A. Bustamante Pineda, Mariona Agramunt, Silvia Byun, Hyang-Min Fernandez, Mariana F. Sunyer Deu, Jordi Estivill, Xavier, 1955- |
| author_role |
author |
| author2 |
Baccarelli, Andrea A. Bustamante Pineda, Mariona Agramunt, Silvia Byun, Hyang-Min Fernandez, Mariana F. Sunyer Deu, Jordi Estivill, Xavier, 1955- |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme |
| topic |
ADN -- Metabolisme ADN -- Metilació Placenta -- Metabolisme |
| description |
AIM: The placenta is an informative and easily available tissue for many epidemiological studies. We analyzed the extent to which storage delay affects DNA methylation. MATERIAL & METHODS: Biopsies from two placentas were sequentially stored at -80°C after standing at room temperature for 30 min, 1 h, 2 h, 6 h and 24 h. Global DNA methylation was measured by bisulfite pyrosequencing of repetitive elements and the luminometric methylation assay. RESULTS: Small changes in global DNA methylation in relation to time-to-storage were observed by pyrosequencing, with a coefficient of variation (COV) of 2.49% (placenta 1) and 2.86% (placenta 2), similar to the mean technical variation observed for pyrosequencing (COV: 1.91 and 1.51%, respectively). A luminometric methylation assay yielded more variable results in the two placentas analyzed, both among time points (COV: 9.13 and 10.35%, respectively) and technical replicates (COV: 11.60 and 9.80%, respectively). CONCLUSION: Global DNA methylation is stable at room temperature. However, some techniques to measure methylation might be confounded by DNA degradation caused by a delay in storage. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013 2019 2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/36592 http://dx.doi.org/10.2217/epi.13.29 |
| url |
http://hdl.handle.net/10230/36592 http://dx.doi.org/10.2217/epi.13.29 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Epigenomics. 2013 Jun;5(3):341-8 |
| dc.rights.none.fl_str_mv |
© 2013 Future Medicine Ltd info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
© 2013 Future Medicine Ltd |
| eu_rights_str_mv |
openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Future Medicine |
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Future Medicine |
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reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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