Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine

Human milk is a crucial source of thyroid hormones, essential for neonatal development. We developed and validated a three-phase hollow-fiber liquid-phase microextraction method, coupled to ultra-high performance liquid chromatography and ion mobility mass spectrometry, to simultaneously quantify ei...

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Detalles Bibliográficos
Autores: Vélez Pérez, Rafael de Fátima, Arias Borrego, Ana, Velasco, Inés, Soldevila, Berta, García Barrera, Tamara
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad de Huelva (UHU)
Repositorio:Arias Montano. Repositorio Institucional de la Universidad de Huelva
Idioma:inglés
OAI Identifier:oai:ariasmontano.uhu.es:10272/27350
Acceso en línea:https://hdl.handle.net/10272/27350
Access Level:acceso abierto
Palabra clave:Hollow-fiber liquid-phase microextraction
Ion mobility
Ultra-high-performance liquid chromatography
Lodine speciation
Quadrupole time of flight
Thyroid hormones
Human milk
2301.03 Análisis Cromatográfico
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spelling Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxineVélez Pérez, Rafael de FátimaArias Borrego, AnaVelasco, InésSoldevila, BertaGarcía Barrera, TamaraHollow-fiber liquid-phase microextractionIon mobilityUltra-high-performance liquid chromatographyLodine speciationQuadrupole time of flightThyroid hormonesHuman milk2301.03 Análisis CromatográficoHuman milk is a crucial source of thyroid hormones, essential for neonatal development. We developed and validated a three-phase hollow-fiber liquid-phase microextraction method, coupled to ultra-high performance liquid chromatography and ion mobility mass spectrometry, to simultaneously quantify eight thyroid hormones, including the chiral forms D-thyroxine and L-thyroxine in human milk. The method showed excellent linearity (R2 > 0.999), low limits of detection (0.7–19.8 μg L-1), and high recoveries (83–114 %). The analysis of 30 human milk samples revealed that D-thyroxine (115.1–157.5 μg L-1) was more abundant than L-thyroxine (47.9–193.6 μg L-1), and reversed triiodothyronine prevailed over triiodothyronine. This is the first report of chiral forms of thyroxine, diiodotirosine, and thyronine in human milk, with potential implications for lipid metabolism and infant thyroid regulation. These findings highlight the method sensitivity and biological relevance, offering a robust tool for future nutritional and endocrine studies.Elsevier20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10272/27350reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/273502026-06-02T14:58:11Z
dc.title.none.fl_str_mv Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
title Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
spellingShingle Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
Vélez Pérez, Rafael de Fátima
Hollow-fiber liquid-phase microextraction
Ion mobility
Ultra-high-performance liquid chromatography
Lodine speciation
Quadrupole time of flight
Thyroid hormones
Human milk
2301.03 Análisis Cromatográfico
title_short Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
title_full Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
title_fullStr Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
title_full_unstemmed Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
title_sort Liquid chromatography (ion mobility) coupled to organic and inorganic mass spectrometry for determination of eight thyroid hormones in human milk with enantiomeric separation of thyroxine
dc.creator.none.fl_str_mv Vélez Pérez, Rafael de Fátima
Arias Borrego, Ana
Velasco, Inés
Soldevila, Berta
García Barrera, Tamara
author Vélez Pérez, Rafael de Fátima
author_facet Vélez Pérez, Rafael de Fátima
Arias Borrego, Ana
Velasco, Inés
Soldevila, Berta
García Barrera, Tamara
author_role author
author2 Arias Borrego, Ana
Velasco, Inés
Soldevila, Berta
García Barrera, Tamara
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Hollow-fiber liquid-phase microextraction
Ion mobility
Ultra-high-performance liquid chromatography
Lodine speciation
Quadrupole time of flight
Thyroid hormones
Human milk
2301.03 Análisis Cromatográfico
topic Hollow-fiber liquid-phase microextraction
Ion mobility
Ultra-high-performance liquid chromatography
Lodine speciation
Quadrupole time of flight
Thyroid hormones
Human milk
2301.03 Análisis Cromatográfico
description Human milk is a crucial source of thyroid hormones, essential for neonatal development. We developed and validated a three-phase hollow-fiber liquid-phase microextraction method, coupled to ultra-high performance liquid chromatography and ion mobility mass spectrometry, to simultaneously quantify eight thyroid hormones, including the chiral forms D-thyroxine and L-thyroxine in human milk. The method showed excellent linearity (R2 > 0.999), low limits of detection (0.7–19.8 μg L-1), and high recoveries (83–114 %). The analysis of 30 human milk samples revealed that D-thyroxine (115.1–157.5 μg L-1) was more abundant than L-thyroxine (47.9–193.6 μg L-1), and reversed triiodothyronine prevailed over triiodothyronine. This is the first report of chiral forms of thyroxine, diiodotirosine, and thyronine in human milk, with potential implications for lipid metabolism and infant thyroid regulation. These findings highlight the method sensitivity and biological relevance, offering a robust tool for future nutritional and endocrine studies.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10272/27350
url https://hdl.handle.net/10272/27350
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva
instname:Universidad de Huelva (UHU)
instname_str Universidad de Huelva (UHU)
reponame_str Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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