Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway

18 páginas.- 6 figuras.- 7 tablas.- 80 referencias.- Supplementary data associated with this article can be found in the online version at doi:10.1016/j.jhazmat.2024.136128

Detalles Bibliográficos
Autores: Lara Moreno, Alba, Vargas-Ordóñez, Antonio, Villaverde Capellán, J., Madrid Díaz, Fernando, Carlier, Jorge D., Santos, Juan Luis, Alonso, Esteban, Morillo González, Esmeralda
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/370408
Acceso en línea:http://hdl.handle.net/10261/370408
Access Level:acceso abierto
Palabra clave:Paracetamol
Sewage sludge
Pseudomonas extremaustralis
Stutzerimonas stutzeri
Biodegradation
Mineralization
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spelling Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathwayLara Moreno, AlbaVargas-Ordóñez, AntonioVillaverde Capellán, J.Madrid Díaz, FernandoCarlier, Jorge D.Santos, Juan LuisAlonso, EstebanMorillo González, EsmeraldaParacetamolSewage sludgePseudomonas extremaustralisStutzerimonas stutzeriBiodegradationMineralization18 páginas.- 6 figuras.- 7 tablas.- 80 referencias.- Supplementary data associated with this article can be found in the online version at doi:10.1016/j.jhazmat.2024.136128Wastewater treatment plants (WWTPs) are recognized as significant contributors of paracetamol (APAP) into the environment due to their limited ability to degrade it. This study used a bioaugmentation strategy with Pseudomonas extremaustralis CSW01 and Stutzerimonas stutzeri CSW02 to achieve APAP biodegradation in solution in wide ranges of temperature (10–40 °C) and pH (5−9), reaching DT50 values < 1.5 h to degrade 500 mg L−1 APAP. Bacterial strains also mineralized APAP in solution (<30 %), but when forming consortia with Mycolicibacterium aubagnense HPB1.1, mineralization significantly increased (up to 74 % and 58 % for CSW01 +HPB1.1 and CSW02 +HPB1.1, respectively), decreasing DT50 values to only 1 and 9 days. Despite the complete degradation of APAP and its high mineralization, residual toxicity throughout the process was observed. Three APAP metabolites were identified (4-aminophenol, hydroquinone and trans-2-hexenoic acid) that quickly disappeared, but residual toxicity remained, indicating the presence of other non-detected intermediates. CSW01 and CSW02 degraded also 100 % APAP (50 mg kg−1) adsorbed on sewage sludge, with DT50 values of only 0.7 and 0.3 days, respectively, but < 15 % APAP was mineralized. A genome-based analysis of CSW01 and CSW02 revealed that amidases, deaminases, hydroxylases, and dioxygenases enzymes were involved in APAP biodegradation, and a possible metabolic pathway was proposed.This work was supported by Junta de Andalucía (Council of Economic Transformation, Industry, Knowledge and University) under the research project PY20_01069 (FEDER Andalucía PO 2014-2020), and by Portuguese National Funds from FCT-Fundação para a Ciência e a Tecnologia, I.P., within the scope of the project PTDC/CTA-AMB/7782/2020. A.L.M. acknowledges the Margarita Salas grant to the University of Seville (funded by the European Union’s Next Generation EU). A.V.O. acknowledges the Youth Employment fellowship (AND21_IRNAS_M2_158) funded by Junta de Andalucía.Peer reviewedElsevierJunta de AndalucíaEuropean CommissionFundação para a Ciência e a Tecnologia (Portugal)Universidad de SevillaLara Moreno, Alba [0000-0002-5741-7662]Vargas-Ordoñez, Antonio [0000-0001-6909-8075]Villaverde Capellán, J. [0000-0002-8694-7929]Madrid Díaz, Fernando [0000-0002-2921-3515]Morillo González, Esmeralda [0000-0002-4485-2315]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/370408reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/Junta de Andalucía/Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020)/PY20_01069http://dx.doi.org/10.1016/j.jhazmat.2024.136128Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3704082026-05-22T06:33:51Z
dc.title.none.fl_str_mv Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway
title Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway
spellingShingle Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway
Lara Moreno, Alba
Paracetamol
Sewage sludge
Pseudomonas extremaustralis
Stutzerimonas stutzeri
Biodegradation
Mineralization
title_short Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway
title_full Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway
title_fullStr Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway
title_full_unstemmed Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway
title_sort Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway
dc.creator.none.fl_str_mv Lara Moreno, Alba
Vargas-Ordóñez, Antonio
Villaverde Capellán, J.
Madrid Díaz, Fernando
Carlier, Jorge D.
Santos, Juan Luis
Alonso, Esteban
Morillo González, Esmeralda
author Lara Moreno, Alba
author_facet Lara Moreno, Alba
Vargas-Ordóñez, Antonio
Villaverde Capellán, J.
Madrid Díaz, Fernando
Carlier, Jorge D.
Santos, Juan Luis
Alonso, Esteban
Morillo González, Esmeralda
author_role author
author2 Vargas-Ordóñez, Antonio
Villaverde Capellán, J.
Madrid Díaz, Fernando
Carlier, Jorge D.
Santos, Juan Luis
Alonso, Esteban
Morillo González, Esmeralda
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
European Commission
Fundação para a Ciência e a Tecnologia (Portugal)
Universidad de Sevilla
Lara Moreno, Alba [0000-0002-5741-7662]
Vargas-Ordoñez, Antonio [0000-0001-6909-8075]
Villaverde Capellán, J. [0000-0002-8694-7929]
Madrid Díaz, Fernando [0000-0002-2921-3515]
Morillo González, Esmeralda [0000-0002-4485-2315]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Paracetamol
Sewage sludge
Pseudomonas extremaustralis
Stutzerimonas stutzeri
Biodegradation
Mineralization
topic Paracetamol
Sewage sludge
Pseudomonas extremaustralis
Stutzerimonas stutzeri
Biodegradation
Mineralization
description 18 páginas.- 6 figuras.- 7 tablas.- 80 referencias.- Supplementary data associated with this article can be found in the online version at doi:10.1016/j.jhazmat.2024.136128
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/370408
url http://hdl.handle.net/10261/370408
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/Junta de Andalucía/Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020)/PY20_01069
http://dx.doi.org/10.1016/j.jhazmat.2024.136128
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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