Evaluating Host Defense Peptides

Saubi, Cristina|||0000-0002-1866-8418; Carratalá, José Vicente|||0000-0001-6950-2939; Bello Madruga, Roberto|||0009-0003-8474-3951; López-Cano, Adrià; Navarro, Susanna|||0000-0001-8160-9536; Arís i Giralt, Anna|||0000-0001-7830-888X; Garcia-Fruitos, Elena|||0000-0001-7498-4864

Evaluating Host Defense Peptides

The global antibiotic resistance crisis raises concerns about antibiotic use, and alternative strategies are urgently needed. In this context, host defense peptides (HDPs) have rapidly gained interest. However, one of the main obstacles is their production strategy. Chemical synthesis is the most wi...

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Autores:	Saubi, Cristina\|\|\|0000-0002-1866-8418, Carratalá, José Vicente\|\|\|0000-0001-6950-2939, Bello Madruga, Roberto\|\|\|0009-0003-8474-3951, López-Cano, Adrià, Navarro, Susanna\|\|\|0000-0001-8160-9536, Arís i Giralt, Anna\|\|\|0000-0001-7830-888X, Garcia-Fruitos, Elena\|\|\|0000-0001-7498-4864
Tipo de recurso:	artículo
Fecha de publicación:	2025
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:318771
Acceso en línea:	https://ddd.uab.cat/record/318771 https://dx.doi.org/urn:doi:10.3390/biom15070980
Access Level:	acceso abierto
Palabra clave:	Host defense peptides Recombinant production Concatemers Antimicrobial activity Lactococcus lactis

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spelling	Evaluating Host Defense PeptidesA Comparative Analysis of Synthetic Peptides and Recombinant ConcatemersSaubi, Cristina\|\|\|0000-0002-1866-8418Carratalá, José Vicente\|\|\|0000-0001-6950-2939Bello Madruga, Roberto\|\|\|0009-0003-8474-3951López-Cano, AdriàNavarro, Susanna\|\|\|0000-0001-8160-9536Arís i Giralt, Anna\|\|\|0000-0001-7830-888XGarcia-Fruitos, Elena\|\|\|0000-0001-7498-4864Host defense peptidesRecombinant productionConcatemersAntimicrobial activityLactococcus lactisThe global antibiotic resistance crisis raises concerns about antibiotic use, and alternative strategies are urgently needed. In this context, host defense peptides (HDPs) have rapidly gained interest. However, one of the main obstacles is their production strategy. Chemical synthesis is the most widely used, although it is not scalable and has sequence limitations. A possible alternative is recombinant production, but the strategies used so far have limited efficiency. In this study, we aim to compare the activity and main characteristics of different HDPs produced by both chemical synthesis and by recombinant production, using an approach based on tetramers to ameliorate the production process. The results obtained showed that the production of HDPs as tetrameric peptides by recombinant production in Lactococcus lactis enhanced the peptide activity, with HDPs being much more active in terms of antimicrobial activity, more structurally stable, and nanostructured. Thus, the recombinant strategy described herein, fusing four repetitions of the same peptide, can become a real alternative to produce highly active HDPs through a scalable production process. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/318771https://dx.doi.org/urn:doi:10.3390/biom15070980reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2022-136521OB-I00Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PRE2021-097678Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-01552open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3187712026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Evaluating Host Defense Peptides A Comparative Analysis of Synthetic Peptides and Recombinant Concatemers
title	Evaluating Host Defense Peptides
spellingShingle	Evaluating Host Defense Peptides Saubi, Cristina\|\|\|0000-0002-1866-8418 Host defense peptides Recombinant production Concatemers Antimicrobial activity Lactococcus lactis
title_short	Evaluating Host Defense Peptides
title_full	Evaluating Host Defense Peptides
title_fullStr	Evaluating Host Defense Peptides
title_full_unstemmed	Evaluating Host Defense Peptides
title_sort	Evaluating Host Defense Peptides
dc.creator.none.fl_str_mv	Saubi, Cristina\|\|\|0000-0002-1866-8418 Carratalá, José Vicente\|\|\|0000-0001-6950-2939 Bello Madruga, Roberto\|\|\|0009-0003-8474-3951 López-Cano, Adrià Navarro, Susanna\|\|\|0000-0001-8160-9536 Arís i Giralt, Anna\|\|\|0000-0001-7830-888X Garcia-Fruitos, Elena\|\|\|0000-0001-7498-4864
author	Saubi, Cristina\|\|\|0000-0002-1866-8418
author_facet	Saubi, Cristina\|\|\|0000-0002-1866-8418 Carratalá, José Vicente\|\|\|0000-0001-6950-2939 Bello Madruga, Roberto\|\|\|0009-0003-8474-3951 López-Cano, Adrià Navarro, Susanna\|\|\|0000-0001-8160-9536 Arís i Giralt, Anna\|\|\|0000-0001-7830-888X Garcia-Fruitos, Elena\|\|\|0000-0001-7498-4864
author_role	author
author2	Carratalá, José Vicente\|\|\|0000-0001-6950-2939 Bello Madruga, Roberto\|\|\|0009-0003-8474-3951 López-Cano, Adrià Navarro, Susanna\|\|\|0000-0001-8160-9536 Arís i Giralt, Anna\|\|\|0000-0001-7830-888X Garcia-Fruitos, Elena\|\|\|0000-0001-7498-4864
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Host defense peptides Recombinant production Concatemers Antimicrobial activity Lactococcus lactis
topic	Host defense peptides Recombinant production Concatemers Antimicrobial activity Lactococcus lactis
description	The global antibiotic resistance crisis raises concerns about antibiotic use, and alternative strategies are urgently needed. In this context, host defense peptides (HDPs) have rapidly gained interest. However, one of the main obstacles is their production strategy. Chemical synthesis is the most widely used, although it is not scalable and has sequence limitations. A possible alternative is recombinant production, but the strategies used so far have limited efficiency. In this study, we aim to compare the activity and main characteristics of different HDPs produced by both chemical synthesis and by recombinant production, using an approach based on tetramers to ameliorate the production process. The results obtained showed that the production of HDPs as tetrameric peptides by recombinant production in Lactococcus lactis enhanced the peptide activity, with HDPs being much more active in terms of antimicrobial activity, more structurally stable, and nanostructured. Thus, the recombinant strategy described herein, fusing four repetitions of the same peptide, can become a real alternative to produce highly active HDPs through a scalable production process.
publishDate	2025
dc.date.none.fl_str_mv	2 2025-01-01 2025 2025-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/318771 https://dx.doi.org/urn:doi:10.3390/biom15070980
url	https://ddd.uab.cat/record/318771 https://dx.doi.org/urn:doi:10.3390/biom15070980
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2022-136521OB-I00 Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 PRE2021-097678 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-01552
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.812429

Evaluating Host Defense Peptides

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