More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses

[EN] Main conclusion Plastoglobules are ubiquitous under non-stress conditions and their morphology, closely related to their composition, changes differently depending on the specific stress that the plant undergoes. Plastoglobules are lipoprotein structures attached to thylakoid membranes, which p...

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Autores: Arzac Garmendia, Miren Irati, Fernández Marín, Beatriz, García Plazaola, José Ignacio
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/55648
Acceso en línea:http://hdl.handle.net/10810/55648
Access Level:acceso abierto
Palabra clave:chloroplast
plastoglobule
stress response
transmission electron microscopy
thylakoid
ultrastructure
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spelling More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responsesArzac Garmendia, Miren IratiFernández Marín, BeatrizGarcía Plazaola, José Ignaciochloroplastplastoglobulestress responsetransmission electron microscopythylakoidultrastructure[EN] Main conclusion Plastoglobules are ubiquitous under non-stress conditions and their morphology, closely related to their composition, changes differently depending on the specific stress that the plant undergoes. Plastoglobules are lipoprotein structures attached to thylakoid membranes, which participate in chloroplast metabolism and stress responses. Their structure contains a coating lipid monolayer and a hydrophobic core that differ in composition. Their function in chloroplasts has been studied focussing on their composition. However, we currently lack a comprehensive study that quantitatively evaluates the occurrence and morphology of plastoglobules. Following a literature search strategy, we quantified the main morphological attributes of plastoglobules from photosynthetic chloroplasts of more than 1000 TEM images published over the last 53 years, covering more than 100 taxa and 15 stress types. The analysis shows that plastoglobules under non-stress conditions are spherical, with an average diameter of 100-200 nm and cover less than 3% of the chloroplast cross-section area. This percentage rises under almost every type of stress, particularly in senescence. Interestingly, an apparent trade-off between increasing either the number or the diameter of plastoglobules governs this response. Our results show that plastoglobules are ubiquitous in chloroplasts of higher plants under non-stress conditions. Besides, provided the specific molecular composition of the core and coat of plastoglobules, we conclude that specific stress-related variation in plastoglobules attributes may allow inferring precise responses of the chloroplast metabolism.This work was supported by the project PGC2018-093824-B-C44 from the Ministerio de Ciencia, Innovación y Universidades (MCIU, Spain) and the European Regional Development Fund (ERDF) and grant UPV/EHU IT-1018-16 from the Basque Government (Spain). MIA received a pre-doctoral grant from the Basque Government. We thank J. Miranda-Apodaca for helpful assistance with PCA analysis. Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature.Springer202220222022info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/55648reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MICIU/PGC2018-093824-B-C44/https://link.springer.com/article/10.1007/s00425-022-03848-9info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Atribución 3.0 Españaoai:addi.ehu.eus:10810/556482026-06-18T09:23:17Z
dc.title.none.fl_str_mv More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses
title More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses
spellingShingle More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses
Arzac Garmendia, Miren Irati
chloroplast
plastoglobule
stress response
transmission electron microscopy
thylakoid
ultrastructure
title_short More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses
title_full More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses
title_fullStr More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses
title_full_unstemmed More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses
title_sort More than just lipid balls: quantitative analysis of plastoglobule attributes and their stress‑related responses
dc.creator.none.fl_str_mv Arzac Garmendia, Miren Irati
Fernández Marín, Beatriz
García Plazaola, José Ignacio
author Arzac Garmendia, Miren Irati
author_facet Arzac Garmendia, Miren Irati
Fernández Marín, Beatriz
García Plazaola, José Ignacio
author_role author
author2 Fernández Marín, Beatriz
García Plazaola, José Ignacio
author2_role author
author
dc.subject.none.fl_str_mv chloroplast
plastoglobule
stress response
transmission electron microscopy
thylakoid
ultrastructure
topic chloroplast
plastoglobule
stress response
transmission electron microscopy
thylakoid
ultrastructure
description [EN] Main conclusion Plastoglobules are ubiquitous under non-stress conditions and their morphology, closely related to their composition, changes differently depending on the specific stress that the plant undergoes. Plastoglobules are lipoprotein structures attached to thylakoid membranes, which participate in chloroplast metabolism and stress responses. Their structure contains a coating lipid monolayer and a hydrophobic core that differ in composition. Their function in chloroplasts has been studied focussing on their composition. However, we currently lack a comprehensive study that quantitatively evaluates the occurrence and morphology of plastoglobules. Following a literature search strategy, we quantified the main morphological attributes of plastoglobules from photosynthetic chloroplasts of more than 1000 TEM images published over the last 53 years, covering more than 100 taxa and 15 stress types. The analysis shows that plastoglobules under non-stress conditions are spherical, with an average diameter of 100-200 nm and cover less than 3% of the chloroplast cross-section area. This percentage rises under almost every type of stress, particularly in senescence. Interestingly, an apparent trade-off between increasing either the number or the diameter of plastoglobules governs this response. Our results show that plastoglobules are ubiquitous in chloroplasts of higher plants under non-stress conditions. Besides, provided the specific molecular composition of the core and coat of plastoglobules, we conclude that specific stress-related variation in plastoglobules attributes may allow inferring precise responses of the chloroplast metabolism.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/55648
url http://hdl.handle.net/10810/55648
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICIU/PGC2018-093824-B-C44/
https://link.springer.com/article/10.1007/s00425-022-03848-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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