Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures

Alonso-Alonso, Sandra|||0000-0001-9873-9134; Esteve-Codina, Anna|||0000-0003-0361-2873; Martin-Mur, Beatriz; Álvarez-González, Lucía|||0000-0001-8154-8614; Ruiz Herrera Moreno, Aurora|||0000-0003-3868-6151; Santaló, Josep|||0000-0002-1698-9699; Ibáñez, Elena|||0000-0002-0275-6958

Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures

Embryonic stem cell (ESC) derivation from single blastomeres of 8-cell mouse embryos results in lower derivation rates than that from whole blastocysts, raising a biological question about the developmental potential of sister blastomeres. We aimed to assess the ability of 8-cell blastomeres to prod...

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Autores:	Alonso-Alonso, Sandra\|\|\|0000-0001-9873-9134, Esteve-Codina, Anna\|\|\|0000-0003-0361-2873, Martin-Mur, Beatriz, Álvarez-González, Lucía\|\|\|0000-0001-8154-8614, Ruiz Herrera Moreno, Aurora\|\|\|0000-0003-3868-6151, Santaló, Josep\|\|\|0000-0002-1698-9699, Ibáñez, Elena\|\|\|0000-0002-0275-6958
Tipo de recurso:	artículo
Fecha de publicación:	2023
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:293977
Acceso en línea:	https://ddd.uab.cat/record/293977 https://dx.doi.org/urn:doi:10.3389/fcell.2023.1274660
Access Level:	acceso abierto
Palabra clave:	Cell fate Developmental potential ESCs Epiblast Pluripotency

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spelling	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signaturesAlonso-Alonso, Sandra\|\|\|0000-0001-9873-9134Esteve-Codina, Anna\|\|\|0000-0003-0361-2873Martin-Mur, BeatrizÁlvarez-González, Lucía\|\|\|0000-0001-8154-8614Ruiz Herrera Moreno, Aurora\|\|\|0000-0003-3868-6151Santaló, Josep\|\|\|0000-0002-1698-9699Ibáñez, Elena\|\|\|0000-0002-0275-6958Cell fateDevelopmental potentialESCsEpiblastPluripotencyEmbryonic stem cell (ESC) derivation from single blastomeres of 8-cell mouse embryos results in lower derivation rates than that from whole blastocysts, raising a biological question about the developmental potential of sister blastomeres. We aimed to assess the ability of 8-cell blastomeres to produce epiblast cells and ESC lines after isolation, and the properties of the resulting lines. Our results revealed unequal competence among sister blastomeres to produce ESC lines. At least half of the blastomeres possess a lower potential to generate ESCs, although culture conditions and blastomeres plasticity can redirect their non-pluripotent fate towards the epiblast lineage, allowing us to generate up to seven lines from the same embryo. Lines originated from the same embryo segregated into two groups according to their transcriptional signatures. While the expression of genes related to pluripotency and development was higher in one group, no differences were found in their trilineage differentiation ability. These results may help to improve our understanding of the ESC derivation process from single blastomeres and cell fate determination in the preimplantation mouse embryos. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/293977https://dx.doi.org/urn:doi:10.3389/fcell.2023.1274660reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00122Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-112557GB-I00Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PRE-2018-083257open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2939772026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures
title	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures
spellingShingle	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures Alonso-Alonso, Sandra\|\|\|0000-0001-9873-9134 Cell fate Developmental potential ESCs Epiblast Pluripotency
title_short	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures
title_full	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures
title_fullStr	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures
title_full_unstemmed	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures
title_sort	Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures
dc.creator.none.fl_str_mv	Alonso-Alonso, Sandra\|\|\|0000-0001-9873-9134 Esteve-Codina, Anna\|\|\|0000-0003-0361-2873 Martin-Mur, Beatriz Álvarez-González, Lucía\|\|\|0000-0001-8154-8614 Ruiz Herrera Moreno, Aurora\|\|\|0000-0003-3868-6151 Santaló, Josep\|\|\|0000-0002-1698-9699 Ibáñez, Elena\|\|\|0000-0002-0275-6958
author	Alonso-Alonso, Sandra\|\|\|0000-0001-9873-9134
author_facet	Alonso-Alonso, Sandra\|\|\|0000-0001-9873-9134 Esteve-Codina, Anna\|\|\|0000-0003-0361-2873 Martin-Mur, Beatriz Álvarez-González, Lucía\|\|\|0000-0001-8154-8614 Ruiz Herrera Moreno, Aurora\|\|\|0000-0003-3868-6151 Santaló, Josep\|\|\|0000-0002-1698-9699 Ibáñez, Elena\|\|\|0000-0002-0275-6958
author_role	author
author2	Esteve-Codina, Anna\|\|\|0000-0003-0361-2873 Martin-Mur, Beatriz Álvarez-González, Lucía\|\|\|0000-0001-8154-8614 Ruiz Herrera Moreno, Aurora\|\|\|0000-0003-3868-6151 Santaló, Josep\|\|\|0000-0002-1698-9699 Ibáñez, Elena\|\|\|0000-0002-0275-6958
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Cell fate Developmental potential ESCs Epiblast Pluripotency
topic	Cell fate Developmental potential ESCs Epiblast Pluripotency
description	Embryonic stem cell (ESC) derivation from single blastomeres of 8-cell mouse embryos results in lower derivation rates than that from whole blastocysts, raising a biological question about the developmental potential of sister blastomeres. We aimed to assess the ability of 8-cell blastomeres to produce epiblast cells and ESC lines after isolation, and the properties of the resulting lines. Our results revealed unequal competence among sister blastomeres to produce ESC lines. At least half of the blastomeres possess a lower potential to generate ESCs, although culture conditions and blastomeres plasticity can redirect their non-pluripotent fate towards the epiblast lineage, allowing us to generate up to seven lines from the same embryo. Lines originated from the same embryo segregated into two groups according to their transcriptional signatures. While the expression of genes related to pluripotency and development was higher in one group, no differences were found in their trilineage differentiation ability. These results may help to improve our understanding of the ESC derivation process from single blastomeres and cell fate determination in the preimplantation mouse embryos.
publishDate	2023
dc.date.none.fl_str_mv	2 2023-01-01 2023 2023-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/293977 https://dx.doi.org/urn:doi:10.3389/fcell.2023.1274660
url	https://ddd.uab.cat/record/293977 https://dx.doi.org/urn:doi:10.3389/fcell.2023.1274660
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.relation.none.fl_str_mv	Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00122 Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-112557GB-I00 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 PRE-2018-083257
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
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Blastomeres of 8-cell mouse embryos differ in their ability to generate embryonic stem cells and produce lines with different transcriptional signatures

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