Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals

This article belongs to the Section Molecular Pharmacology.

Detalhes bibliográficos
Autores: Gyenes, Andrea, Tapasztó, Zsófia, Quirce, Susana, Luna, Carolina, Frutos-Rincón, Laura, Gallar, Juana, Acosta, M. Carmen, Kovács, Illés
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/352970
Acesso em linha:http://hdl.handle.net/10261/352970
Access Level:acceso abierto
Palavra-chave:Dry eye
Cold thermoreceptors
Cyclosporine A
Corneal nerves
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spelling Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminalsGyenes, AndreaTapasztó, ZsófiaQuirce, SusanaLuna, CarolinaFrutos-Rincón, LauraGallar, JuanaAcosta, M. CarmenKovács, IllésDry eyeCold thermoreceptorsCyclosporine ACorneal nervesThis article belongs to the Section Molecular Pharmacology.Cyclosporine A (CsA) is used for the treatment of dry eye (DE) with good clinical results, improving tear secretion and decreasing subjective symptoms. These effects are attributed to the improved tear film dynamics, but there are no data on the effect of CsA on the abnormal sensory nerve activity characteristic in DE. Our purpose was to evaluate the CsA effect on the enhanced activity of corneal cold thermoreceptors in a tear-deficient DE animal model using in vitro extracellular recording of cold thermoreceptors nerve terminal impulses (NTIs) before and in the presence of CsA. NTI shape was also analyzed. Blinking frequency and tearing rate were also measured in awake animals before and after topical CsA. CsA increased the tearing and blinking of treated animals. CsA significantly decreased the peak response to cold of cold thermoreceptors. Neither their spontaneous NTIs discharge rate nor their cooling threshold were modified. CsA also seemed to reverse some of the changes in NTI shape induced by tear deficiency. These data suggest that, at least in part, the beneficial clinical effects of CsA in DE can be attributed to a direct effect on sensory nerve endings, although the precise mechanisms underlying this effect need further studies to be fully clarified.This work was supported by grant PID2020-115934RB-I00 (JG and MCA) and predoctoral fellowship BES-2015-072638 (SQ), financed by the Spanish Agencia Estatal de Investigación (MCIN/AEI/10.13039/501100011033), grant CIPROM/2021/48 (JG) and predoctoral fellowship ACIF/2017/169 (LF-R) from the Generalitat Valenciana, Spain, and by the Hungarian Scientific Research Fund—European FP7 Marie Curie Mobility grant Human-MB08A 80372 and OTKA NN106649 (IK).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Ministerio de Ciencia e Innovación (España)Generalitat ValencianaAgencia Estatal de Investigación (España)European CommissionMinisterio de Economía y Competitividad (España)Hungarian Scientific Research FundConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/352970reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115934RB-I00info:eu-repo/grantAgreement/MINECO//BES-2015-072638info:eu-repo/grantAgreement/EC/FP7/80372https://doi.org/10.3390/ijms241613025Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3529702026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals
title Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals
spellingShingle Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals
Gyenes, Andrea
Dry eye
Cold thermoreceptors
Cyclosporine A
Corneal nerves
title_short Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals
title_full Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals
title_fullStr Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals
title_full_unstemmed Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals
title_sort Cyclosporine A decreases dryness-induced hyperexcitability of corneal cold-sensitive nerve terminals
dc.creator.none.fl_str_mv Gyenes, Andrea
Tapasztó, Zsófia
Quirce, Susana
Luna, Carolina
Frutos-Rincón, Laura
Gallar, Juana
Acosta, M. Carmen
Kovács, Illés
author Gyenes, Andrea
author_facet Gyenes, Andrea
Tapasztó, Zsófia
Quirce, Susana
Luna, Carolina
Frutos-Rincón, Laura
Gallar, Juana
Acosta, M. Carmen
Kovács, Illés
author_role author
author2 Tapasztó, Zsófia
Quirce, Susana
Luna, Carolina
Frutos-Rincón, Laura
Gallar, Juana
Acosta, M. Carmen
Kovács, Illés
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Ciencia e Innovación (España)
Generalitat Valenciana
Agencia Estatal de Investigación (España)
European Commission
Ministerio de Economía y Competitividad (España)
Hungarian Scientific Research Fund
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Dry eye
Cold thermoreceptors
Cyclosporine A
Corneal nerves
topic Dry eye
Cold thermoreceptors
Cyclosporine A
Corneal nerves
description This article belongs to the Section Molecular Pharmacology.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/352970
url http://hdl.handle.net/10261/352970
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115934RB-I00
info:eu-repo/grantAgreement/MINECO//BES-2015-072638
info:eu-repo/grantAgreement/EC/FP7/80372
https://doi.org/10.3390/ijms241613025
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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