Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection

© 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credi...

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Autores: Casais, Rosa, Granda García, Víctor, Balseiro Morales, Ana María, Cerro, Ana del, Dalton, Kevin P., González, Roxana, Bravo, Pablo, Prieto, J. M., Montoya, María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/19900
Acceso en línea:https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1717-9
https://hdl.handle.net/10612/19900
Access Level:acceso abierto
Palabra clave:Inmunología
Sanidad animal
Veterinaria
Sarcoptic mange
Sarcoptes scabiei
Immunodominant antigens
Vaccine candidates
Clinical protection
3109 Ciencias Veterinarias
3109.03 Inmunología
id ES_05b5b40ba60c4ece8b7216013fa810d0
oai_identifier_str oai:buleria.unileon.es:10612/19900
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection
title Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection
spellingShingle Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection
Casais, Rosa
Inmunología
Sanidad animal
Veterinaria
Sarcoptic mange
Sarcoptes scabiei
Immunodominant antigens
Vaccine candidates
Clinical protection
3109 Ciencias Veterinarias
3109.03 Inmunología
title_short Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection
title_full Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection
title_fullStr Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection
title_full_unstemmed Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection
title_sort Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection
dc.creator.none.fl_str_mv Casais, Rosa
Granda García, Víctor
Balseiro Morales, Ana María
Cerro, Ana del
Dalton, Kevin P.
González, Roxana
Bravo, Pablo
Prieto, J. M.
Montoya, María
author Casais, Rosa
author_facet Casais, Rosa
Granda García, Víctor
Balseiro Morales, Ana María
Cerro, Ana del
Dalton, Kevin P.
González, Roxana
Bravo, Pablo
Prieto, J. M.
Montoya, María
author_role author
author2 Granda García, Víctor
Balseiro Morales, Ana María
Cerro, Ana del
Dalton, Kevin P.
González, Roxana
Bravo, Pablo
Prieto, J. M.
Montoya, María
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sanidad Animal
Facultad de Veterinaria
dc.subject.none.fl_str_mv Inmunología
Sanidad animal
Veterinaria
Sarcoptic mange
Sarcoptes scabiei
Immunodominant antigens
Vaccine candidates
Clinical protection
3109 Ciencias Veterinarias
3109.03 Inmunología
topic Inmunología
Sanidad animal
Veterinaria
Sarcoptic mange
Sarcoptes scabiei
Immunodominant antigens
Vaccine candidates
Clinical protection
3109 Ciencias Veterinarias
3109.03 Inmunología
description © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1717-9
https://hdl.handle.net/10612/19900
url https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1717-9
https://hdl.handle.net/10612/19900
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2010-22200-C02-01
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protectionCasais, RosaGranda García, VíctorBalseiro Morales, Ana MaríaCerro, Ana delDalton, Kevin P.González, RoxanaBravo, PabloPrieto, J. M.Montoya, MaríaInmunologíaSanidad animalVeterinariaSarcoptic mangeSarcoptes scabieiImmunodominant antigensVaccine candidatesClinical protection3109 Ciencias Veterinarias3109.03 Inmunología© 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.[EN]Background: Vaccination is an attractive ecological alternative to the use of acaricides for parasite control. However, effective anti-parasite vaccines against sarcoptic mange have not yet been developed. The purpose of this study was first to identify Sarcoptes scabiei immunodominant antigens and second to evaluate them as vaccine candidates in a rabbit/S. scabiei var. cuniculi model. Methods: The S. scabiei Ssλ15 immunodominant antigen was selected by immunoscreening of a S. scabiei var. hominis cDNA. The full-length cDNA was sequenced and cloned into the pGEX vector and the recombinant protein expressed in BL21 (DE3) cells and purified. A vaccination trial was performed consisting of a test group (n = 8) immunised with recAgs (a mix of two recombinant antigens, Ssλ15 and the previously described Ssλ20ΔB3) and a control group (n = 8) immunised with PBS. All analyses were performed with R Statistical Environment with α set at 0.050. Results: The full-length open reading frame of the 1,821 nt cloned cDNA encodes a 64 kDa polypeptide, the sequence of which had 96 % identity with a hypothetical protein of S. scabiei. Ssλ15 was localised by immunostaining of skin sections in the tegument surrounding the mouthparts and the coxa in the legs of mites. Rabbit immunisation with recAgs induced high levels of specific IgG (P < 0.010) and increased levels of total IgEs. However, no significant clinical protection against S. scabiei challenge was detected. Unexpectedly, the group immunised with the recAgs mix had significantly higher lesion scores (P = 0.050) although lower mean mite densities than those observed in the control group. These results might indicate that the lesions in the recAgs group were due not only to the mites density but also to an exacerbated immunological response after challenge, which is in agreement with the specific high levels of pro-inflammatory cytokines (IL-1 and TNFα) detected after challenge in this group. Conclusions: The selected antigens delivered as recombinant proteins had no clinical protective efficacy against S. scabiei infestation although immunisation reduced mite density. However, these results pave the way for future studies on alternative production systems, adjuvants, delivery methods and combinations of antigens in order to manage stimulation of clinical protective immune responses.SIThis work was partially funded by grant RTA11-00087-00-00 from the Spanish Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Fondo Europeo de Desarrollo Regional (FEDER), AGL2010-22200-C02-01 from Spanish Ministry (MINECO) and the Biotechnology and Biological Sciences Research Council (BBSRC) grant BBS/E/I/00002014.BMCSanidad AnimalFacultad de Veterinaria2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1717-9https://hdl.handle.net/10612/19900reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2010-22200-C02-01http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/199002026-06-24T12:43:27Z
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