Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies

13 p.-2 fig.

Detalles Bibliográficos
Autores: Kamal, Khaled Y., van Loon, Jack JWA, Medina, F. Javier, Herranz, Raúl
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/205529
Acceso en línea:http://hdl.handle.net/10261/205529
Access Level:acceso abierto
Palabra clave:Simulated microgravity
Ground-based facilities
Arabidopsis thaliana
Suspension cell culture
Immobilized cells
Agarose embedding
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spelling Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studiesKamal, Khaled Y.van Loon, Jack JWAMedina, F. JavierHerranz, RaúlSimulated microgravityGround-based facilitiesArabidopsis thalianaSuspension cell cultureImmobilized cellsAgarose embedding13 p.-2 fig.Gravity plays a role in modulating plant growth and development and its alteration induces changes in these processes. Microgravity research has recently been extended to the use of in vitro plant cell cultures which are considered as an ideal model system to study cell proliferation and growth. In general, among the ground-based facilities available for microgravity simulation, the 2D pipette clinostat had been previously considered a suitable facility to be used for unicellular biological models although studies using single plant cell cultures raised some concerns. The incompatibility comes from the standard requirement of shaking a suspension culture for assuring its viability and active proliferation status in the control samples. Moreover, a related issue applies to the use of the random positioning machine (RPM) for cell suspension experiments. Here, we demonstrate an alternative culture method based on the immobilization of the culture before the altered gravity treatment occurs, such that it behaves as a solid object. Our immobilization procedure preserved plant cell culture viability without compromising basic cell properties as viability, morphology, cell cycle phases distribution, or chromatin organization, when compared with a standard cell suspension under shaking as a control. This approach should allow the space biology community to improve the quantity and quality of plant cell results in future simulated microgravity experiments or spaceflight opportunities.This work was supported by grants of the Spanish National Plan for Research and Development, Ref. Nos.AYA2012-33982 and ESP2015-64323-R, the Dutch Space Research Organization (NWOALW-SRON) [Grant number MG-057] and the ESA-ELIPS Program[ESA SEGMGSPE Ph1 Project, contract number 4200022650]. KYK was supported by the Spanish CSIC JAE-PreDoc Program (Ref.JAEPre 2010 01894).Peer reviewedSpringer NatureMinisterio de Economía y Competitividad (España)Netherlands Institute for Space ResearchEuropean CommissionConsejo Superior de Investigaciones Científicas (España)Kamal, Khaled Y. [0000-0002-6909-8056]van Loon, Jack JWA [0000-0001-9051-6016]Medina, F. Javier [0000-0002-0866-7710]Herranz, Raúl [0000-0002-0246-9449]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/205529reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/ESP2015-64323-Rhttps://doi.org/10.1007/s12217-016-9531-8Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2055292026-05-22T06:33:51Z
dc.title.none.fl_str_mv Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies
title Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies
spellingShingle Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies
Kamal, Khaled Y.
Simulated microgravity
Ground-based facilities
Arabidopsis thaliana
Suspension cell culture
Immobilized cells
Agarose embedding
title_short Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies
title_full Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies
title_fullStr Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies
title_full_unstemmed Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies
title_sort Embedding arabidopsis plant cell suspensions in low-melting agarose facilitates altered gravity studies
dc.creator.none.fl_str_mv Kamal, Khaled Y.
van Loon, Jack JWA
Medina, F. Javier
Herranz, Raúl
author Kamal, Khaled Y.
author_facet Kamal, Khaled Y.
van Loon, Jack JWA
Medina, F. Javier
Herranz, Raúl
author_role author
author2 van Loon, Jack JWA
Medina, F. Javier
Herranz, Raúl
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Netherlands Institute for Space Research
European Commission
Consejo Superior de Investigaciones Científicas (España)
Kamal, Khaled Y. [0000-0002-6909-8056]
van Loon, Jack JWA [0000-0001-9051-6016]
Medina, F. Javier [0000-0002-0866-7710]
Herranz, Raúl [0000-0002-0246-9449]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Simulated microgravity
Ground-based facilities
Arabidopsis thaliana
Suspension cell culture
Immobilized cells
Agarose embedding
topic Simulated microgravity
Ground-based facilities
Arabidopsis thaliana
Suspension cell culture
Immobilized cells
Agarose embedding
description 13 p.-2 fig.
publishDate 2017
dc.date.none.fl_str_mv 2017
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/205529
url http://hdl.handle.net/10261/205529
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/ESP2015-64323-R
https://doi.org/10.1007/s12217-016-9531-8
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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