Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils

The presence of tens of Clostridium botulinum spores per gram of honey can cause infantile botulism. Thermal treatment is insufficient to inactivate these resistant forms. This study explored the effectiveness of supercritical CO2 (scCO2 ) on its own and combined with lemon (LEO), clove (CLEO), and...

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Detalles Bibliográficos
Autores: Dacal Gutierrez, Alejandro, Tirado Armesto, Diego Felipe, Calvo Garrido, María Lourdes
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/72971
Acceso en línea:https://hdl.handle.net/20.500.14352/72971
Access Level:acceso abierto
Palabra clave:66.0
high-pressure carbon dioxide
lemon essential oil
clove essential oil
cinnamon essential oil
Clostridium sporogenes
Ingeniería química
3303 Ingeniería y Tecnología Químicas
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oai_identifier_str oai:docta.ucm.es:20.500.14352/72971
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network_name_str España
repository_id_str
spelling Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential OilsDacal Gutierrez, AlejandroTirado Armesto, Diego FelipeCalvo Garrido, María Lourdes66.0high-pressure carbon dioxidelemon essential oilclove essential oilcinnamon essential oilClostridium sporogenesIngeniería química3303 Ingeniería y Tecnología QuímicasThe presence of tens of Clostridium botulinum spores per gram of honey can cause infantile botulism. Thermal treatment is insufficient to inactivate these resistant forms. This study explored the effectiveness of supercritical CO2 (scCO2 ) on its own and combined with lemon (LEO), clove (CLEO), and cinnamon (CEO) essential oils on the inactivation of Clostridium sporogenes (CECT 553) as a surrogate of Clostridium botulinum. In water, the degree of inactivation at 10 MPa after 60 min increased with the increasing temperature, reducing the population by 90% at 40 ◦C and by 99.7% at 80 ◦C. In contrast, when applied to honey, scCO2 did not inactivate Clostridium spores satisfactorily at temperatures below 70 ◦C, which was related to the protective effect of honey. Meanwhile, scCO2 modified with CEOMDPIUniversidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/72971reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/729712026-06-02T12:44:21Z
dc.title.none.fl_str_mv Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils
title Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils
spellingShingle Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils
Dacal Gutierrez, Alejandro
66.0
high-pressure carbon dioxide
lemon essential oil
clove essential oil
cinnamon essential oil
Clostridium sporogenes
Ingeniería química
3303 Ingeniería y Tecnología Químicas
title_short Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils
title_full Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils
title_fullStr Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils
title_full_unstemmed Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils
title_sort Inactivation of Clostridium Spores in Honey with Supercritical CO2 and in Combination with Essential Oils
dc.creator.none.fl_str_mv Dacal Gutierrez, Alejandro
Tirado Armesto, Diego Felipe
Calvo Garrido, María Lourdes
author Dacal Gutierrez, Alejandro
author_facet Dacal Gutierrez, Alejandro
Tirado Armesto, Diego Felipe
Calvo Garrido, María Lourdes
author_role author
author2 Tirado Armesto, Diego Felipe
Calvo Garrido, María Lourdes
author2_role author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 66.0
high-pressure carbon dioxide
lemon essential oil
clove essential oil
cinnamon essential oil
Clostridium sporogenes
Ingeniería química
3303 Ingeniería y Tecnología Químicas
topic 66.0
high-pressure carbon dioxide
lemon essential oil
clove essential oil
cinnamon essential oil
Clostridium sporogenes
Ingeniería química
3303 Ingeniería y Tecnología Químicas
description The presence of tens of Clostridium botulinum spores per gram of honey can cause infantile botulism. Thermal treatment is insufficient to inactivate these resistant forms. This study explored the effectiveness of supercritical CO2 (scCO2 ) on its own and combined with lemon (LEO), clove (CLEO), and cinnamon (CEO) essential oils on the inactivation of Clostridium sporogenes (CECT 553) as a surrogate of Clostridium botulinum. In water, the degree of inactivation at 10 MPa after 60 min increased with the increasing temperature, reducing the population by 90% at 40 ◦C and by 99.7% at 80 ◦C. In contrast, when applied to honey, scCO2 did not inactivate Clostridium spores satisfactorily at temperatures below 70 ◦C, which was related to the protective effect of honey. Meanwhile, scCO2 modified with CEO
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/72971
url https://hdl.handle.net/20.500.14352/72971
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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