Identification of proximal SUMO-dependent interactors using SUMO-ID

[EN]The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation by TurboID and protein-fragment complementation to find SUMO-dependent interactors of...

ver descrição completa

Detalhes bibliográficos
Autores: Barroso Gomila, Orhi, Trulsson, Erik, Muratore, Veronica, Canosa, Iñigo, Merino Cacho, Laura, Cortázar, Ana Rosa, Pérez Fernández, Coralia, Azkargorta, Mikel, Iloro, Ibon, Carracedo Pérez, Arkaitz, Aransay Bañares, Ana María, Elortza, Felix, Mayor Martínez, Ugo, Verteegal, Alfred C. O., Barrio Olano, María Rosa, Sutherland, James D.
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/54398
Acesso em linha:http://hdl.handle.net/10810/54398
Access Level:acceso abierto
Palavra-chave:ubiquitin
SUMO-ID
id ES_0534ee9aa3fdade70ae2966d86e83ceb
oai_identifier_str oai:addi.ehu.eus:10810/54398
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Identification of proximal SUMO-dependent interactors using SUMO-ID
title Identification of proximal SUMO-dependent interactors using SUMO-ID
spellingShingle Identification of proximal SUMO-dependent interactors using SUMO-ID
Barroso Gomila, Orhi
ubiquitin
SUMO-ID
title_short Identification of proximal SUMO-dependent interactors using SUMO-ID
title_full Identification of proximal SUMO-dependent interactors using SUMO-ID
title_fullStr Identification of proximal SUMO-dependent interactors using SUMO-ID
title_full_unstemmed Identification of proximal SUMO-dependent interactors using SUMO-ID
title_sort Identification of proximal SUMO-dependent interactors using SUMO-ID
dc.creator.none.fl_str_mv Barroso Gomila, Orhi
Trulsson, Erik
Muratore, Veronica
Canosa, Iñigo
Merino Cacho, Laura
Cortázar, Ana Rosa
Pérez Fernández, Coralia
Azkargorta, Mikel
Iloro, Ibon
Carracedo Pérez, Arkaitz
Aransay Bañares, Ana María
Elortza, Felix
Mayor Martínez, Ugo
Verteegal, Alfred C. O.
Barrio Olano, María Rosa
Sutherland, James D.
author Barroso Gomila, Orhi
author_facet Barroso Gomila, Orhi
Trulsson, Erik
Muratore, Veronica
Canosa, Iñigo
Merino Cacho, Laura
Cortázar, Ana Rosa
Pérez Fernández, Coralia
Azkargorta, Mikel
Iloro, Ibon
Carracedo Pérez, Arkaitz
Aransay Bañares, Ana María
Elortza, Felix
Mayor Martínez, Ugo
Verteegal, Alfred C. O.
Barrio Olano, María Rosa
Sutherland, James D.
author_role author
author2 Trulsson, Erik
Muratore, Veronica
Canosa, Iñigo
Merino Cacho, Laura
Cortázar, Ana Rosa
Pérez Fernández, Coralia
Azkargorta, Mikel
Iloro, Ibon
Carracedo Pérez, Arkaitz
Aransay Bañares, Ana María
Elortza, Felix
Mayor Martínez, Ugo
Verteegal, Alfred C. O.
Barrio Olano, María Rosa
Sutherland, James D.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
dc.subject.none.fl_str_mv ubiquitin
SUMO-ID
topic ubiquitin
SUMO-ID
description [EN]The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation by TurboID and protein-fragment complementation to find SUMO-dependent interactors of proteins of interest. We develop an optimized split-TurboID version and show SUMO interaction-dependent labelling of proteins proximal to PML and RANGAP1. SUMO-dependent interactors of PML are involved in transcription, DNA damage, stress response and SUMO modification and are highly enriched in SUMO Interacting Motifs, but may only represent a subset of the total PML proximal proteome. Likewise, SUMO-ID also allow us to identify interactors of SUMOylated SALL1, a less characterized SUMO substrate. Furthermore, using TP53 as a substrate, we identify SUMO1, SUMO2 and Ubiquitin preferential interactors. Thus, SUMO-ID is a powerful tool that allows to study the consequences of SUMO-dependent interactions, and may further unravel the complexity of the ubiquitin code.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/54398
url http://hdl.handle.net/10810/54398
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/EC/H2020/765445
info:eu-repo/grantAgreement/MINECO/SAF2017-90900-REDT/
info:eu-repo/grantAgreement/MINECO/BFU2017-84653-P/
info:eu-repo/grantAgreement/MICIU/SEV-2016-0644/
info:eu-repo/grantAgreement/MICIU/PRE2018-086230/
info:eu-repo/grantAgreement/MICINN/PID2020-114178GB-I00/
info:eu-repo/grantAgreement/MICINN/PID2019-108787RB-I00/
info:eu-repo/grantAgreement/MICIU/RED2018-102769-T/
info:eu-repo/grantAgreement/EC/FP7/336343
info:eu-repo/grantAgreement/EC/H2020/754627
info:eu-repo/grantAgreement/EC/H2020/819242
info:eu-repo/grantAgreement/MINECO/SAF2016-76898-P/
https://www-nature-com.ehu.idm.oclc.org/articles/s41467-021-26807-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402840354521088
spelling Identification of proximal SUMO-dependent interactors using SUMO-IDBarroso Gomila, OrhiTrulsson, ErikMuratore, VeronicaCanosa, IñigoMerino Cacho, LauraCortázar, Ana RosaPérez Fernández, CoraliaAzkargorta, MikelIloro, IbonCarracedo Pérez, ArkaitzAransay Bañares, Ana MaríaElortza, FelixMayor Martínez, UgoVerteegal, Alfred C. O.Barrio Olano, María RosaSutherland, James D.ubiquitinSUMO-ID[EN]The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation by TurboID and protein-fragment complementation to find SUMO-dependent interactors of proteins of interest. We develop an optimized split-TurboID version and show SUMO interaction-dependent labelling of proteins proximal to PML and RANGAP1. SUMO-dependent interactors of PML are involved in transcription, DNA damage, stress response and SUMO modification and are highly enriched in SUMO Interacting Motifs, but may only represent a subset of the total PML proximal proteome. Likewise, SUMO-ID also allow us to identify interactors of SUMOylated SALL1, a less characterized SUMO substrate. Furthermore, using TP53 as a substrate, we identify SUMO1, SUMO2 and Ubiquitin preferential interactors. Thus, SUMO-ID is a powerful tool that allows to study the consequences of SUMO-dependent interactions, and may further unravel the complexity of the ubiquitin code.We are thankful to Iraide Escobes for her work in the Proteomics Platform at CIC bioGUNE and Arnoud de Ru in the Center for Proteomics and Metabolomics at the LUMC. O.B.-G., F.T., R.B. and A.C.O.V. acknowledge funding by the grant 765445-EU (UbiCODE Program). R.B. acknowledges funding by grants BFU2017-84653-P and PID2020-114178GB-I00 (MINECO/FEDER, EU), SEV-2016-0644 (Severo Ochoa Excellence Program), SAF2017-90900-REDT (UBIRed Program) and IT1165-19 (Basque Country Government). Additional support was provided by the Department of Industry, Tourism, and Trade of the Basque Country Government (Elkartek Research Programs) and by the Innovation Technology Department of the Bizkaia County. VM acknowledges FPI grant PRE2018-086230 (MINECO/FEDER, EU). F.E. is at Proteomics Platform, member of ProteoRed-ISCIII (PT13/0001/0027). F.E. and A.M.A. acknowledge CIBERehd. A.C. acknowledges the Basque Department of education (IKERTALDE IT1106-16), the MCIU (PID2019-108787RB-I00 (FEDER/EU), Severo Ochoa Excellence Accreditation SEV-2016-0644, Excellence Networks RED2018-102769-T), the AECC (GCTRA18006CARR), La Caixa Foundation (ID 100010434), under the agreement LCF/PR/HR17, and the European Research Council (Starting Grant 336343, PoC 754627, Consolidator grant 819242). CIBERONC was co-funded with FEDER funds. U.M. acknowledges the Basque Government Department of Education (IT1165-19) and the Spanish MCIU (SAF2016-76898-P (FEDER/EU)).Nature ResearchEuropean Commission202120212021info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/54398reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/EC/H2020/765445info:eu-repo/grantAgreement/MINECO/SAF2017-90900-REDT/info:eu-repo/grantAgreement/MINECO/BFU2017-84653-P/info:eu-repo/grantAgreement/MICIU/SEV-2016-0644/info:eu-repo/grantAgreement/MICIU/PRE2018-086230/info:eu-repo/grantAgreement/MICINN/PID2020-114178GB-I00/info:eu-repo/grantAgreement/MICINN/PID2019-108787RB-I00/info:eu-repo/grantAgreement/MICIU/RED2018-102769-T/info:eu-repo/grantAgreement/EC/FP7/336343info:eu-repo/grantAgreement/EC/H2020/754627info:eu-repo/grantAgreement/EC/H2020/819242info:eu-repo/grantAgreement/MINECO/SAF2016-76898-P/https://www-nature-com.ehu.idm.oclc.org/articles/s41467-021-26807-6info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Atribución 3.0 Españaoai:addi.ehu.eus:10810/543982026-06-18T09:23:17Z
score 15,198674

Registos relacionados