A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast
Splicing is an important step of gene expression regulation in eukaryotes, as there are many mRNA precursors that can be alternatively spliced in different tissues, at different cell cycle phases or under different external stimuli. We have developed several integrated fluorescence-based in vivo spl...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/60777 |
| Acceso en línea: | http://hdl.handle.net/10230/60777 http://dx.doi.org/10.1371/journal.pgen.1011316 |
| Access Level: | acceso abierto |
| Palabra clave: | Introns Schizosaccharomyces pombe DNA transcription Gene regulation Gene expression Yellow fluorescent protein RNA splicing Saccharomyces cerevisiae |
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A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeastBorao Arriazu, Sonia, 1993-Vega, MontserratBoronat i Llop, Susanna, 1965-Hidalgo Hernando, ElenaHümmer, StefanAyté del Olmo, JoséIntronsSchizosaccharomyces pombeDNA transcriptionGene regulationGene expressionYellow fluorescent proteinRNA splicingSaccharomyces cerevisiaeSplicing is an important step of gene expression regulation in eukaryotes, as there are many mRNA precursors that can be alternatively spliced in different tissues, at different cell cycle phases or under different external stimuli. We have developed several integrated fluorescence-based in vivo splicing reporter constructs that allow the quantification of fission yeast splicing in vivo on intact cells, and we have compared their splicing efficiency in a wild type strain and in a prp2-1 (U2AF65) genetic background, showing a clear dependency between Prp2 and a consensus signal at 5' splicing site (5'SS). To isolate novel genes involved in regulated splicing, we have crossed the reporter showing more intron retention with the Schizosaccharomyces pombe knock out collection. Among the candidate genes involved in the regulation of splicing, we have detected strong splicing defects in two of the mutants -Δcwf12, a member of the NineTeen Complex (NTC) and Δsaf5, a methylosome subunit that acts together with the survival motor neuron (SMN) complex in small nuclear ribonucleoproteins (snRNP) biogenesis. We have identified that strains with mutations in cwf12 have inefficient splicing, mainly when the 5'SS differs from the consensus. However, although Δsaf5 cells also have some dependency on 5'SS sequence, we noticed that when one intron of a given pre-mRNA was affected, the rest of the introns of the same pre-mRNA had high probabilities of being also affected. This observation points Saf5 as a link between transcription rate and splicing.This work was supported by grants BFU2018-PGC2018-097248-B-I00 and PID2022-136449NB-I00 funded by MICIU/AEI/10.13039/501100011033 and ERDF/EU to JA and by Unidad de Excelencia Maria de Maeztu (CEX2018-000792-M) to JA and EH. EH is a recipient of an ICREA Academia Award (Generalitat de Catalunya). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of Science (PLoS)202420242024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/60777http://dx.doi.org/10.1371/journal.pgen.1011316reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS Genet. 2024 Jun 4;20(6):e1011316info:eu-repo/grantAgreement/ES/2PE/PGC2018-097248-B-I00info:eu-repo/grantAgreement/ES/3PE/PID2022-136449NB-I00info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M© 2024 Borao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/607772026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast |
| title |
A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast |
| spellingShingle |
A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast Borao Arriazu, Sonia, 1993- Introns Schizosaccharomyces pombe DNA transcription Gene regulation Gene expression Yellow fluorescent protein RNA splicing Saccharomyces cerevisiae |
| title_short |
A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast |
| title_full |
A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast |
| title_fullStr |
A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast |
| title_full_unstemmed |
A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast |
| title_sort |
A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast |
| dc.creator.none.fl_str_mv |
Borao Arriazu, Sonia, 1993- Vega, Montserrat Boronat i Llop, Susanna, 1965- Hidalgo Hernando, Elena Hümmer, Stefan Ayté del Olmo, José |
| author |
Borao Arriazu, Sonia, 1993- |
| author_facet |
Borao Arriazu, Sonia, 1993- Vega, Montserrat Boronat i Llop, Susanna, 1965- Hidalgo Hernando, Elena Hümmer, Stefan Ayté del Olmo, José |
| author_role |
author |
| author2 |
Vega, Montserrat Boronat i Llop, Susanna, 1965- Hidalgo Hernando, Elena Hümmer, Stefan Ayté del Olmo, José |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Introns Schizosaccharomyces pombe DNA transcription Gene regulation Gene expression Yellow fluorescent protein RNA splicing Saccharomyces cerevisiae |
| topic |
Introns Schizosaccharomyces pombe DNA transcription Gene regulation Gene expression Yellow fluorescent protein RNA splicing Saccharomyces cerevisiae |
| description |
Splicing is an important step of gene expression regulation in eukaryotes, as there are many mRNA precursors that can be alternatively spliced in different tissues, at different cell cycle phases or under different external stimuli. We have developed several integrated fluorescence-based in vivo splicing reporter constructs that allow the quantification of fission yeast splicing in vivo on intact cells, and we have compared their splicing efficiency in a wild type strain and in a prp2-1 (U2AF65) genetic background, showing a clear dependency between Prp2 and a consensus signal at 5' splicing site (5'SS). To isolate novel genes involved in regulated splicing, we have crossed the reporter showing more intron retention with the Schizosaccharomyces pombe knock out collection. Among the candidate genes involved in the regulation of splicing, we have detected strong splicing defects in two of the mutants -Δcwf12, a member of the NineTeen Complex (NTC) and Δsaf5, a methylosome subunit that acts together with the survival motor neuron (SMN) complex in small nuclear ribonucleoproteins (snRNP) biogenesis. We have identified that strains with mutations in cwf12 have inefficient splicing, mainly when the 5'SS differs from the consensus. However, although Δsaf5 cells also have some dependency on 5'SS sequence, we noticed that when one intron of a given pre-mRNA was affected, the rest of the introns of the same pre-mRNA had high probabilities of being also affected. This observation points Saf5 as a link between transcription rate and splicing. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024 2024 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/60777 http://dx.doi.org/10.1371/journal.pgen.1011316 |
| url |
http://hdl.handle.net/10230/60777 http://dx.doi.org/10.1371/journal.pgen.1011316 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
PLoS Genet. 2024 Jun 4;20(6):e1011316 info:eu-repo/grantAgreement/ES/2PE/PGC2018-097248-B-I00 info:eu-repo/grantAgreement/ES/3PE/PID2022-136449NB-I00 info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M |
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http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Public Library of Science (PLoS) |
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Public Library of Science (PLoS) |
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reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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