The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation

Liquid storage is the primary preservation method in the swine breeding industry because of its advantages over cryopreservation. Calcium (Ca2+), a key regulator of cell physiology, plays a crucial role during liquid preservation. Sarcoplasmic/Endoplasmic Reticulum Ca2+ ATPases (SERCA) belong to a f...

Descripción completa

Detalles Bibliográficos
Autores: Garriga, Ferran, Martínez-Hernández, Jesús., Parra-Balaguer, Ainhoa, Llavanera, Marc, Yeste Oliveras, Marc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/26476
Acceso en línea:http://hdl.handle.net/10256/26476
Access Level:acceso abierto
Palabra clave:Citometria de fluxe
Flow cytometry
Semen -- Conservació
Semen -- Conservation
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
id ES_04cbb2f0dedddaba96f666f44f455850
oai_identifier_str oai:recercat.cat:10256/26476
network_acronym_str ES
network_name_str España
repository_id_str
spelling The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservationGarriga, FerranMartínez-Hernández, Jesús.Parra-Balaguer, AinhoaLlavanera, MarcYeste Oliveras, MarcCitometria de fluxeFlow cytometrySemen -- ConservacióSemen -- ConservationPorcs -- Espermatozoides -- InvestigacióSwine -- Spermatozoa -- ResearchLiquid storage is the primary preservation method in the swine breeding industry because of its advantages over cryopreservation. Calcium (Ca2+), a key regulator of cell physiology, plays a crucial role during liquid preservation. Sarcoplasmic/Endoplasmic Reticulum Ca2+ ATPases (SERCA) belong to a family of P-type ATPases that regulate Ca2+ homeostasis within cells and have been previously described to play a function in the sperm of various mammalian species. Herein, we hypothesized that SERCA2 is present in pig sperm and is involved in the resilience of this cell to liquid preservation at 17 °C. For this purpose, sperm were incubated with different concentrations of thapsigargin (Thg; 0, 5, 25, and 50 µM) and stored at 17 °C for ten days. The presence and localization of SERCA2 were evaluated using immunoblotting and immunofluorescence, respectively. On days 0, 4, and 10, sperm motility was assessed using a computer-assisted sperm analysis (CASA) system, and sperm viability, membrane lipid disorder, acrosome integrity, mitochondrial membrane potential (MMP), and intracellular levels of Ca2+, superoxides and total reactive oxygen species (ROS) were evaluated by flow cytometry. We localized SERCA2 in the acrosome and midpiece of pig sperm. Furthermore, inhibition of SERCA with Thg resulted in reduced sperm viability and membrane stability, and increased MMP, and Ca2+ and ROS levels. In conclusion, the activity of SERCA prevents the accumulation of intracellular Ca2+ in sperm, which is detrimental to sperm quality and function during liquid storage at 17 °C. We thus suggest that the function of SERCA is crucial for the preservation of pig semenThis study was supported by the Ministry of Science, Innovation and Universities, Spain (grants: PID2020-113320RB-I00 and PRE2021-098896), the European Union-Next Generation EU Funds (University of Murcia, Margarita Salas Scheme 181/MSJD/22), the Regional Government of Catalonia (2021-SGR-0900), and the Catalan Institution for Research and Advanced Studies (ICREA)Nature Publishing GroupAgencia Estatal de Investigación2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewed1.01999999999953 p.application/pdfhttp://hdl.handle.net/10256/26476http://hdl.handle.net/10256/26476Scientific Reports, 2025, vol. 15, art.núm.4184, p. 4184.1-4184.12Articles publicats (D-B)Garriga, Ferran Martínez-Hernández, Jesús. Parra-Balaguer, Ainhoa Llavanera, Marc Yeste Oliveras, Marc 2025 The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation Scientific Reports 15 art.núm.4184 4184.1 4184.12reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-025-88012-5info:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113320RB-I00Attribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/264762026-05-29T05:05:01Z
dc.title.none.fl_str_mv The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation
title The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation
spellingShingle The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation
Garriga, Ferran
Citometria de fluxe
Flow cytometry
Semen -- Conservació
Semen -- Conservation
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
title_short The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation
title_full The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation
title_fullStr The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation
title_full_unstemmed The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation
title_sort The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation
dc.creator.none.fl_str_mv Garriga, Ferran
Martínez-Hernández, Jesús.
Parra-Balaguer, Ainhoa
Llavanera, Marc
Yeste Oliveras, Marc
author Garriga, Ferran
author_facet Garriga, Ferran
Martínez-Hernández, Jesús.
Parra-Balaguer, Ainhoa
Llavanera, Marc
Yeste Oliveras, Marc
author_role author
author2 Martínez-Hernández, Jesús.
Parra-Balaguer, Ainhoa
Llavanera, Marc
Yeste Oliveras, Marc
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación
dc.subject.none.fl_str_mv Citometria de fluxe
Flow cytometry
Semen -- Conservació
Semen -- Conservation
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
topic Citometria de fluxe
Flow cytometry
Semen -- Conservació
Semen -- Conservation
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
description Liquid storage is the primary preservation method in the swine breeding industry because of its advantages over cryopreservation. Calcium (Ca2+), a key regulator of cell physiology, plays a crucial role during liquid preservation. Sarcoplasmic/Endoplasmic Reticulum Ca2+ ATPases (SERCA) belong to a family of P-type ATPases that regulate Ca2+ homeostasis within cells and have been previously described to play a function in the sperm of various mammalian species. Herein, we hypothesized that SERCA2 is present in pig sperm and is involved in the resilience of this cell to liquid preservation at 17 °C. For this purpose, sperm were incubated with different concentrations of thapsigargin (Thg; 0, 5, 25, and 50 µM) and stored at 17 °C for ten days. The presence and localization of SERCA2 were evaluated using immunoblotting and immunofluorescence, respectively. On days 0, 4, and 10, sperm motility was assessed using a computer-assisted sperm analysis (CASA) system, and sperm viability, membrane lipid disorder, acrosome integrity, mitochondrial membrane potential (MMP), and intracellular levels of Ca2+, superoxides and total reactive oxygen species (ROS) were evaluated by flow cytometry. We localized SERCA2 in the acrosome and midpiece of pig sperm. Furthermore, inhibition of SERCA with Thg resulted in reduced sperm viability and membrane stability, and increased MMP, and Ca2+ and ROS levels. In conclusion, the activity of SERCA prevents the accumulation of intracellular Ca2+ in sperm, which is detrimental to sperm quality and function during liquid storage at 17 °C. We thus suggest that the function of SERCA is crucial for the preservation of pig semen
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/26476
http://hdl.handle.net/10256/26476
url http://hdl.handle.net/10256/26476
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-025-88012-5
info:eu-repo/semantics/altIdentifier/issn/2045-2322
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113320RB-I00
dc.rights.none.fl_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1.01999999999953 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Scientific Reports, 2025, vol. 15, art.núm.4184, p. 4184.1-4184.12
Articles publicats (D-B)
Garriga, Ferran Martínez-Hernández, Jesús. Parra-Balaguer, Ainhoa Llavanera, Marc Yeste Oliveras, Marc 2025 The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation Scientific Reports 15 art.núm.4184 4184.1 4184.12
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402815762268160
score 15,811543

Similares en LA Referencia