Flow cytometric minimal residual disease assessment in B-cell precursor acute lymphoblastic leukaemia patients treated with CD19-targeted therapies — a EuroFlow study

The standardized EuroFlow protocol, including CD19 as primary B-cell marker, enables highly sensitive and reliable minimal residual disease (MRD) assessment in B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) patients treated with chemotherapy. We developed and validated an alternative gatin...

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Detalles Bibliográficos
Autores: Verbeek, Martijn W. C., Buracchi, Chiara, Laqua, Anna, Nierkens, Stefan, Sedek, Lukasz, Flores-Montero, Juan, Hofmans, Mattias, Costa, Elaine S., Novákova, Michaela, Mejstrikova, Ester, Barrena, Susana, Kohlscheen, Saskia, Szczepanowski, Monika, Kulis, Jan, Oliveira, Elen, Jugooa, Romana, Jong, Anja X. de, Szczepanski, Tomasz, Philippé, J., Dongen, J. J. M. van, Orfao, Alberto, Brüggemann, Monika, Gaipa, Giuseppe, Velden, Vincent H. J. van der
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/282850
Acceso en línea:http://hdl.handle.net/10261/282850
Access Level:acceso abierto
Palabra clave:Acute leukaemia
Diagnostic haematology
Flow cytometry
Minimal residual disease
Descripción
Sumario:The standardized EuroFlow protocol, including CD19 as primary B-cell marker, enables highly sensitive and reliable minimal residual disease (MRD) assessment in B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) patients treated with chemotherapy. We developed and validated an alternative gating strategy allowing reliable MRD analysis in BCP-ALL patients treated with CD19-targeting therapies. Concordant data were obtained in 92% of targeted therapy patients who remained CD19-positive, whereas this was 81% in patients that became (partially) CD19-negative. Nevertheless, in both groups median MRD values showed excellent correlation with the original MRD data, indicating that, despite higher interlaboratory variation, the overall MRD analysis was correct.

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