Three independent signaling pathways repress motility in Pseudomonas fluorescens F113

La investigación fue financiada por la beca BIO2006-08596 del Ministerio de Ciencia e Innovación (MICINN) y el programa de investigación MICROAMBIENTE-CM de la Comunidad de Madrid.

Detalles Bibliográficos
Autores: Navazo, Ana, Barahona, Emma, Redondo-Nieto, Miguel, Martínez-Granero, Francisco, Rivilla, Rafael, Martín, Marta
Tipo de recurso: artículo
Fecha de publicación:2009
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
OAI Identifier:oai:burjcdigital.urjc.es:10115/27848
Acceso en línea:https://hdl.handle.net/10115/27848
Access Level:acceso abierto
Palabra clave:Motility
rhizosphere-colonizing
GacA/ GacS two-component system
Epistasis
cyclic di-GMP turnover
phenotypic variants
swimming
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spelling Three independent signaling pathways repress motility in Pseudomonas fluorescens F113Navazo, AnaBarahona, EmmaRedondo-Nieto, MiguelMartínez-Granero, FranciscoRivilla, RafaelMartín, MartaMotilityrhizosphere-colonizingGacA/ GacS two-component systemEpistasiscyclic di-GMP turnoverphenotypic variantsswimmingLa investigación fue financiada por la beca BIO2006-08596 del Ministerio de Ciencia e Innovación (MICINN) y el programa de investigación MICROAMBIENTE-CM de la Comunidad de Madrid.Motility is one of the most important traits for rhizosphere colonization by pseudomonads. Despite this importance, motility is severely repressed in the rhizosphere-colonizing strain Pseudomonas fluorescens F113. This bacterium is unable to swarm under laboratory conditions and produce relatively small swimming haloes. However, phenotypic variants with the ability to swarm and producing swimming haloes up to 300% larger than the wild-type strain, arise during rhizosphere colonization. These variants harbour mutations in the genes encoding the GacA/ GacS two-component system and in other genes. In order to identify genes and pathways implicated in motility repression, we have used generalized mutagenesis with transposons. Analysis of the mutants has shown that besides the Gac system, the Wsp system and the sadB gene, which have been previously implicated in cyclic di-GMP turnover, are implicated in motility repression: mutants in the gacS, sadB or wspR genes can swarm and produce swimming haloes larger than the wild-type strain. Epistasis analysis has shown that the pathways defined by each of these genes are independent, because double and triple mutants show an additive phenotype. Furthermore, GacS, SadB and WspR act at different levels. Expression of the fleQ gene, encoding the master regulator of flagella synthesis is higher in the gacS- and sadB- backgrounds than in the wild-type strain and this differential expression is reflected by a higher secretion of the flagellin protein FliC. Conversely, no differences in fleQ expression or FliC secretion were observed between the wild-type strain and the wspR- mutant.Applied Microbiology International202320232009info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10115/27848reponame:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlosinstname:Universidad Rey Juan CarlosInglésAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:burjcdigital.urjc.es:10115/278482026-06-24T12:48:17Z
dc.title.none.fl_str_mv Three independent signaling pathways repress motility in Pseudomonas fluorescens F113
title Three independent signaling pathways repress motility in Pseudomonas fluorescens F113
spellingShingle Three independent signaling pathways repress motility in Pseudomonas fluorescens F113
Navazo, Ana
Motility
rhizosphere-colonizing
GacA/ GacS two-component system
Epistasis
cyclic di-GMP turnover
phenotypic variants
swimming
title_short Three independent signaling pathways repress motility in Pseudomonas fluorescens F113
title_full Three independent signaling pathways repress motility in Pseudomonas fluorescens F113
title_fullStr Three independent signaling pathways repress motility in Pseudomonas fluorescens F113
title_full_unstemmed Three independent signaling pathways repress motility in Pseudomonas fluorescens F113
title_sort Three independent signaling pathways repress motility in Pseudomonas fluorescens F113
dc.creator.none.fl_str_mv Navazo, Ana
Barahona, Emma
Redondo-Nieto, Miguel
Martínez-Granero, Francisco
Rivilla, Rafael
Martín, Marta
author Navazo, Ana
author_facet Navazo, Ana
Barahona, Emma
Redondo-Nieto, Miguel
Martínez-Granero, Francisco
Rivilla, Rafael
Martín, Marta
author_role author
author2 Barahona, Emma
Redondo-Nieto, Miguel
Martínez-Granero, Francisco
Rivilla, Rafael
Martín, Marta
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Motility
rhizosphere-colonizing
GacA/ GacS two-component system
Epistasis
cyclic di-GMP turnover
phenotypic variants
swimming
topic Motility
rhizosphere-colonizing
GacA/ GacS two-component system
Epistasis
cyclic di-GMP turnover
phenotypic variants
swimming
description La investigación fue financiada por la beca BIO2006-08596 del Ministerio de Ciencia e Innovación (MICINN) y el programa de investigación MICROAMBIENTE-CM de la Comunidad de Madrid.
publishDate 2009
dc.date.none.fl_str_mv 2009
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10115/27848
url https://hdl.handle.net/10115/27848
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Applied Microbiology International
publisher.none.fl_str_mv Applied Microbiology International
dc.source.none.fl_str_mv reponame:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
collection BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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