Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines

In recent years the terms "micro-/nanoplastics" (MNPLs) have caught special attention due to the increasing levels by which humans are exposed. Among MNPLs, polystyrene nanoparticles (PSNPs) are one of the most represented MNPLs in the environment. These tiny particles may enter into the h...

Descripción completa

Detalles Bibliográficos
Autores: Rubio Lorente, Laura|||0000-0002-2088-3803, Barguilla, Irene|||0000-0003-1081-4457, Domenech, Josefa|||0000-0003-1375-0604, Marcos Dauder, Ricardo|||0000-0001-7891-357X, Hernández Bonilla, Alba|||0000-0001-6938-1233
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:325707
Acceso en línea:https://ddd.uab.cat/record/325707
https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2020.122900
Access Level:acceso abierto
Palabra clave:Polystyrene nanoparticles
ROS
Genotoxicity
THP-1
Raji-B
TK6
id ES_04ab4eabc669f86599b410c772d5cbbc
oai_identifier_str oai:ddd.uab.cat:325707
network_acronym_str ES
network_name_str España
repository_id_str
spelling Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell linesRubio Lorente, Laura|||0000-0002-2088-3803Barguilla, Irene|||0000-0003-1081-4457Domenech, Josefa|||0000-0003-1375-0604Marcos Dauder, Ricardo|||0000-0001-7891-357XHernández Bonilla, Alba|||0000-0001-6938-1233Polystyrene nanoparticlesROSGenotoxicityTHP-1Raji-BTK6In recent years the terms "micro-/nanoplastics" (MNPLs) have caught special attention due to the increasing levels by which humans are exposed. Among MNPLs, polystyrene nanoparticles (PSNPs) are one of the most represented MNPLs in the environment. These tiny particles may enter into the human body, translocate through human barriers, interacting with blood and lymphatic immune cells, and reaching secondary organs. By using three different human leukocytic cell lines: Raji-B (B-lymphocytes), TK6 (lymphoblasts) and THP-1 (monocytes), we pursued to determine the effects of these PSNPs on the immune cell population. With this aim, the three cell lines were exposed to spherical PSNPs of about 50 nm of diameter and cytotoxicity, cellular uptake, reactive oxygen species (ROS) production, and genotoxicity were assessed at different time-points. Results show differences in all the measured endpoints, among the selected cell lines. Thus, whilst the monocytic THP-1 cells showed the highest particle internalization, no adverse effects were observed in such cells. On the other side, although Raji-B and TK6 showed lesser PSNPs uptake, mild toxicity, ROS production and genotoxicity were detected. These results highlight the importance of the cell line selection when the biological effects of PSNPs are evaluated. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/325707https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2020.122900reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3257072026-06-06T12:50:31Z
dc.title.none.fl_str_mv Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
title Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
spellingShingle Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
Rubio Lorente, Laura|||0000-0002-2088-3803
Polystyrene nanoparticles
ROS
Genotoxicity
THP-1
Raji-B
TK6
title_short Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
title_full Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
title_fullStr Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
title_full_unstemmed Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
title_sort Biological effects, including oxidative stress and genotoxic damage, of polystyrene nanoparticles in different human hematopoietic cell lines
dc.creator.none.fl_str_mv Rubio Lorente, Laura|||0000-0002-2088-3803
Barguilla, Irene|||0000-0003-1081-4457
Domenech, Josefa|||0000-0003-1375-0604
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
author Rubio Lorente, Laura|||0000-0002-2088-3803
author_facet Rubio Lorente, Laura|||0000-0002-2088-3803
Barguilla, Irene|||0000-0003-1081-4457
Domenech, Josefa|||0000-0003-1375-0604
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
author_role author
author2 Barguilla, Irene|||0000-0003-1081-4457
Domenech, Josefa|||0000-0003-1375-0604
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
author2_role author
author
author
author
dc.subject.none.fl_str_mv Polystyrene nanoparticles
ROS
Genotoxicity
THP-1
Raji-B
TK6
topic Polystyrene nanoparticles
ROS
Genotoxicity
THP-1
Raji-B
TK6
description In recent years the terms "micro-/nanoplastics" (MNPLs) have caught special attention due to the increasing levels by which humans are exposed. Among MNPLs, polystyrene nanoparticles (PSNPs) are one of the most represented MNPLs in the environment. These tiny particles may enter into the human body, translocate through human barriers, interacting with blood and lymphatic immune cells, and reaching secondary organs. By using three different human leukocytic cell lines: Raji-B (B-lymphocytes), TK6 (lymphoblasts) and THP-1 (monocytes), we pursued to determine the effects of these PSNPs on the immune cell population. With this aim, the three cell lines were exposed to spherical PSNPs of about 50 nm of diameter and cytotoxicity, cellular uptake, reactive oxygen species (ROS) production, and genotoxicity were assessed at different time-points. Results show differences in all the measured endpoints, among the selected cell lines. Thus, whilst the monocytic THP-1 cells showed the highest particle internalization, no adverse effects were observed in such cells. On the other side, although Raji-B and TK6 showed lesser PSNPs uptake, mild toxicity, ROS production and genotoxicity were detected. These results highlight the importance of the cell line selection when the biological effects of PSNPs are evaluated.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/325707
https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2020.122900
url https://ddd.uab.cat/record/325707
https://dx.doi.org/urn:doi:10.1016/j.jhazmat.2020.122900
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402806952132608
score 15,811543

Similares en LA Referencia