Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals

The developing cardiovascular system of zebrafish is a sensitive target for many environmental pollutants, including dioxin-like compounds and pesticides. Some polychlorinated biphenyls (PCBs) can compromise the cardiovascular endothelial function by activating oxidative stress-sensitive signaling p...

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Authors: Teixidó Condomines, Elisabet, Barenys Espadaler, Marta, Piqué Benages, Maria Esther, Llobet Mallafré, Joan M. (Joan Maria), Gómez Catalán, Jesús
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/165764
Online Access:https://hdl.handle.net/2445/165764
Access Level:Open access
Keyword:Embriologia
Fenotip
Farmacologia
Toxicologia
Estrès oxidatiu
Peix zebra
Embryology
Phenotype
Pharmacology
Toxicology
Oxidative stress
Zebra danio
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oai_identifier_str oai:diposit.ub.edu:2445/165764
network_acronym_str ES
network_name_str España
repository_id_str
spelling Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicalsTeixidó Condomines, ElisabetBarenys Espadaler, MartaPiqué Benages, Maria EstherLlobet Mallafré, Joan M. (Joan Maria)Gómez Catalán, JesúsEmbriologiaFenotipFarmacologiaToxicologiaEstrès oxidatiuPeix zebraEmbryologyPhenotypePharmacologyToxicologyOxidative stressZebra danioThe developing cardiovascular system of zebrafish is a sensitive target for many environmental pollutants, including dioxin-like compounds and pesticides. Some polychlorinated biphenyls (PCBs) can compromise the cardiovascular endothelial function by activating oxidative stress-sensitive signaling pathways. Therefore, we exposed zebrafish embryos to PCB126 or to several redox-modulating chemicals to study their ability to modulate the dysmorphogenesis produced by PCB126. PCB126 produced a concentration-dependent induction of pericardial edema and circulatory failure, and a concentration-dependent reduction of cardiac output and body length at 80 hours post fertilization (hpf). Among several modulators tested, the effects of PCB126 could be both positively and negatively modulated by different compounds; co-treatment with -tocopherol (vitamin E liposoluble) prevented the adverse effects of PCB126 in pericardial edema, whereas co-treatment with sodium nitroprusside (a vasodilator compound) significantly worsened PCB126 effects. Gene expression analysis showed an up-regulation of cyp1a, hsp70, and gstp1, indicative of PCB126 interaction with the aryl hydrocarbon receptor (AhR), while the transcription of antioxidant genes (sod1, sod2; cat and gpx1a) was not affected. Further studies are necessary to understand the role of oxidative stress in the developmental toxicity of low concentrations of PCB126 (25 nM). Our results give insights into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of environmental pollutants.MDPI2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/165764Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/ijms20051065International Journal of Molecular Sciences, 2019, vol. 20, num. 5, p. 1065https://doi.org/10.3390/ijms20051065cc-by (c) Teixidó Condomines, Elisabet et al., 2019http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1657642026-05-27T06:46:51Z
dc.title.none.fl_str_mv Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals
title Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals
spellingShingle Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals
Teixidó Condomines, Elisabet
Embriologia
Fenotip
Farmacologia
Toxicologia
Estrès oxidatiu
Peix zebra
Embryology
Phenotype
Pharmacology
Toxicology
Oxidative stress
Zebra danio
title_short Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals
title_full Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals
title_fullStr Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals
title_full_unstemmed Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals
title_sort Cardiovascular effects of PCB 126 (3,3',4,4',5-pentachlorobiphenyl) in zebrafish embryos and impact of co-exposure to redox modulating chemicals
dc.creator.none.fl_str_mv Teixidó Condomines, Elisabet
Barenys Espadaler, Marta
Piqué Benages, Maria Esther
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
author Teixidó Condomines, Elisabet
author_facet Teixidó Condomines, Elisabet
Barenys Espadaler, Marta
Piqué Benages, Maria Esther
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
author_role author
author2 Barenys Espadaler, Marta
Piqué Benages, Maria Esther
Llobet Mallafré, Joan M. (Joan Maria)
Gómez Catalán, Jesús
author2_role author
author
author
author
dc.subject.none.fl_str_mv Embriologia
Fenotip
Farmacologia
Toxicologia
Estrès oxidatiu
Peix zebra
Embryology
Phenotype
Pharmacology
Toxicology
Oxidative stress
Zebra danio
topic Embriologia
Fenotip
Farmacologia
Toxicologia
Estrès oxidatiu
Peix zebra
Embryology
Phenotype
Pharmacology
Toxicology
Oxidative stress
Zebra danio
description The developing cardiovascular system of zebrafish is a sensitive target for many environmental pollutants, including dioxin-like compounds and pesticides. Some polychlorinated biphenyls (PCBs) can compromise the cardiovascular endothelial function by activating oxidative stress-sensitive signaling pathways. Therefore, we exposed zebrafish embryos to PCB126 or to several redox-modulating chemicals to study their ability to modulate the dysmorphogenesis produced by PCB126. PCB126 produced a concentration-dependent induction of pericardial edema and circulatory failure, and a concentration-dependent reduction of cardiac output and body length at 80 hours post fertilization (hpf). Among several modulators tested, the effects of PCB126 could be both positively and negatively modulated by different compounds; co-treatment with -tocopherol (vitamin E liposoluble) prevented the adverse effects of PCB126 in pericardial edema, whereas co-treatment with sodium nitroprusside (a vasodilator compound) significantly worsened PCB126 effects. Gene expression analysis showed an up-regulation of cyp1a, hsp70, and gstp1, indicative of PCB126 interaction with the aryl hydrocarbon receptor (AhR), while the transcription of antioxidant genes (sod1, sod2; cat and gpx1a) was not affected. Further studies are necessary to understand the role of oxidative stress in the developmental toxicity of low concentrations of PCB126 (25 nM). Our results give insights into the use of zebrafish embryos for exploring mechanisms underlying the oxidative potential of environmental pollutants.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/165764
url https://hdl.handle.net/2445/165764
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms20051065
International Journal of Molecular Sciences, 2019, vol. 20, num. 5, p. 1065
https://doi.org/10.3390/ijms20051065
dc.rights.none.fl_str_mv cc-by (c) Teixidó Condomines, Elisabet et al., 2019
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Teixidó Condomines, Elisabet et al., 2019
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869402800839983104
score 15,300719

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