Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)

The effects of partial (75%; diet PP75) and total (100%; diet PP100) replacement of fish meal by plant protein sources on the intestinal nutrient absorption of gilthead sea bream and rainbow trout were examined over a 12-week growth trial. The diets comprised a mixture of plant ingredients (corn glu...

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Autores: Santigosa, Ester, García-Meilán, Irene, Valentín, Juana María, Pérez-Sánchez, Jaume, Médale, Françoise, Kaushik, Sadasivam, Gallardo, María Ángeles
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/52097
Acceso en línea:http://hdl.handle.net/10261/52097
Access Level:acceso abierto
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spelling Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)Santigosa, EsterGarcía-Meilán, IreneValentín, Juana MaríaPérez-Sánchez, JaumeMédale, FrançoiseKaushik, SadasivamGallardo, María ÁngelesThe effects of partial (75%; diet PP75) and total (100%; diet PP100) replacement of fish meal by plant protein sources on the intestinal nutrient absorption of gilthead sea bream and rainbow trout were examined over a 12-week growth trial. The diets comprised a mixture of plant ingredients (corn gluten meal, wheat gluten, extruded peas and rapeseed meal) and met the amino acid requirements of the fish. A third group of fish on a fish meal diet was used as a control for comparative purposes. Diets were tested in triplicate and fish were fed twice a day until visual satiation. At the end of the trial, we measured amino acid (l-leucine, l-lysine, l-phenylalanine, l-alanine and l-proline) and d-glucose absorption at short-term (6. h) and long-term (36. h and 48. h in rainbow trout and sea bream respectively) post-feeding by means of brush border membrane vesicles obtained from pyloric caeca, proximal intestine and distal intestine. The absorption pattern at 6. h post-feeding was modified in both species in response to fish meal replacement. In PP75-fed trout absorption was delayed from pyloric caeca to proximal and/or distal intestinal segments, thus total absorption capacity was maintained in this group. On the contrary, total uptake was significantly decreased in trout fed the PP100 diet and in sea bream on both PP75 and PP100 diets. Glucose transport capacity was increased in both experimental sea bream groups and in PP75 trout. Long-term transport capacity was up-regulated for both species. Our results show that intestinal nutrient absorption is modified in response to the use of high levels of plant protein sources and that these changes are species-specific. © 2011 Elsevier B.V.This research was funded by the EU (Q5RS-2000-30068; “Perspectives of Plant Protein Use in Aquaculture”, PEPPA).Peer ReviewedElsevier2012201220112012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/52097reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/520972026-05-22T06:33:51Z
dc.title.none.fl_str_mv Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)
title Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)
spellingShingle Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)
Santigosa, Ester
title_short Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)
title_full Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)
title_fullStr Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)
title_full_unstemmed Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)
title_sort Modifications of intestinal nutrient absorption in response to dietary fish meal replacement by plant protein sources in sea bream (Sparus aurata) and rainbow trout (Onchorynchus mykiss)
dc.creator.none.fl_str_mv Santigosa, Ester
García-Meilán, Irene
Valentín, Juana María
Pérez-Sánchez, Jaume
Médale, Françoise
Kaushik, Sadasivam
Gallardo, María Ángeles
author Santigosa, Ester
author_facet Santigosa, Ester
García-Meilán, Irene
Valentín, Juana María
Pérez-Sánchez, Jaume
Médale, Françoise
Kaushik, Sadasivam
Gallardo, María Ángeles
author_role author
author2 García-Meilán, Irene
Valentín, Juana María
Pérez-Sánchez, Jaume
Médale, Françoise
Kaushik, Sadasivam
Gallardo, María Ángeles
author2_role author
author
author
author
author
author
description The effects of partial (75%; diet PP75) and total (100%; diet PP100) replacement of fish meal by plant protein sources on the intestinal nutrient absorption of gilthead sea bream and rainbow trout were examined over a 12-week growth trial. The diets comprised a mixture of plant ingredients (corn gluten meal, wheat gluten, extruded peas and rapeseed meal) and met the amino acid requirements of the fish. A third group of fish on a fish meal diet was used as a control for comparative purposes. Diets were tested in triplicate and fish were fed twice a day until visual satiation. At the end of the trial, we measured amino acid (l-leucine, l-lysine, l-phenylalanine, l-alanine and l-proline) and d-glucose absorption at short-term (6. h) and long-term (36. h and 48. h in rainbow trout and sea bream respectively) post-feeding by means of brush border membrane vesicles obtained from pyloric caeca, proximal intestine and distal intestine. The absorption pattern at 6. h post-feeding was modified in both species in response to fish meal replacement. In PP75-fed trout absorption was delayed from pyloric caeca to proximal and/or distal intestinal segments, thus total absorption capacity was maintained in this group. On the contrary, total uptake was significantly decreased in trout fed the PP100 diet and in sea bream on both PP75 and PP100 diets. Glucose transport capacity was increased in both experimental sea bream groups and in PP75 trout. Long-term transport capacity was up-regulated for both species. Our results show that intestinal nutrient absorption is modified in response to the use of high levels of plant protein sources and that these changes are species-specific. © 2011 Elsevier B.V.
publishDate 2011
dc.date.none.fl_str_mv 2011
2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/52097
url http://hdl.handle.net/10261/52097
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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