Contribution of gut microbial lysine to liver and milk amino acids in lactating does

The contribution of microbial amino acids through caecotrophy to tissue protein metabolism was investigated in lactating does. Attempts were made to vary microbial supply through a dietary antibiotic, Zn bacitracin, and to vary tissue demand through manipulation of litter size. Three groups of eight...

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Autores: Abecia, Leticia, Balcells Terés, Joaquim, Fondevila, Manuel, Belenguer, Álvaro, Holtrop, G., Lobley, Gerald E.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2008
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/64879
Acceso en línea:https://doi.org/10.1017/S0007114508957986
http://hdl.handle.net/10459.1/64879
Access Level:acceso abierto
Palabra clave:Caecotrophy
Rabbit does
Microbial lysine
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spelling Contribution of gut microbial lysine to liver and milk amino acids in lactating doesAbecia, LeticiaBalcells Terés, JoaquimFondevila, ManuelBelenguer, ÁlvaroHoltrop, G.Lobley, Gerald E.CaecotrophyRabbit doesMicrobial lysineThe contribution of microbial amino acids through caecotrophy to tissue protein metabolism was investigated in lactating does. Attempts were made to vary microbial supply through a dietary antibiotic, Zn bacitracin, and to vary tissue demand through manipulation of litter size. Three groups of eight New Zealand does were fed different experimental diets from day 28 of pregnancy to day 26 of lactation. The control group received the basal diet formulated to meet requirements with grass hay, wheat, soyabean meal and barley grain. The second (no antibiotic) group and the third (bacitracin; BAC) group ingested the basal diet supplemented with ammonium sulfate (5 g/kg), initially unlabelled (day 1 to day 8) then labelled with 15N (day 9 to day 30), while the BAC diet was also supplemented throughout with antibiotic (Zn bacitracin; 100 mg/kg). From just after birth each group of does was subdivided into two groups, each of four females, with the litter size either five (LS5) or nine (LS9) pups. The 15N enrichment in liver, milk and caecal bacteria amino acids was determined by GC-combustion-isotope ratio MS. All amino acids in bacterial protein were enriched with the (15NH4)2SO4 treatment, with lysine 15N enrichment significantly greater in caecal bacteria (0·23 (SE 0·0063) atom % excess (ape)) than in liver (0·04 (SE 0·0004) ape) or milk protein (0·05 (SE 0·0018) ape), confirming the double origin (bacterial and dietary) of tissue lysine. The contribution of microbes to tissue lysine was 0·23 (SE 0·006) when milk protein was used as reference.Work was financed by the Dirección General de Aragón (DGA), through the Research Project reference PM 095/2006. L. A. was funded by a doctoral fellowship (Programa de Formación de Investigadores del Departamento de Educación, Universidades e Investigación del Gobierno Vasco) and by a Marie Curie Training site award (HPMT-CT-2001-00409) during her stage at the Rowett Research Institute. Part of the present study was funded by core grants to the Rowett Research Institute, and Biomathematics and Statistics Scotland (BioSS) by the Scottish Executive.Cambridge University Press2008info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1017/S0007114508957986http://hdl.handle.net/10459.1/64879reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a https://doi.org/10.1017/S0007114508957986British Journal of Nutrition, 2008, vol. 100, núm. 5, p. 977-983(c) Cambridge University Press, 2008info:eu-repo/semantics/openAccessoai:repositori.udl.cat:10459.1/648792026-06-24T12:42:17Z
dc.title.none.fl_str_mv Contribution of gut microbial lysine to liver and milk amino acids in lactating does
title Contribution of gut microbial lysine to liver and milk amino acids in lactating does
spellingShingle Contribution of gut microbial lysine to liver and milk amino acids in lactating does
Abecia, Leticia
Caecotrophy
Rabbit does
Microbial lysine
title_short Contribution of gut microbial lysine to liver and milk amino acids in lactating does
title_full Contribution of gut microbial lysine to liver and milk amino acids in lactating does
title_fullStr Contribution of gut microbial lysine to liver and milk amino acids in lactating does
title_full_unstemmed Contribution of gut microbial lysine to liver and milk amino acids in lactating does
title_sort Contribution of gut microbial lysine to liver and milk amino acids in lactating does
dc.creator.none.fl_str_mv Abecia, Leticia
Balcells Terés, Joaquim
Fondevila, Manuel
Belenguer, Álvaro
Holtrop, G.
Lobley, Gerald E.
author Abecia, Leticia
author_facet Abecia, Leticia
Balcells Terés, Joaquim
Fondevila, Manuel
Belenguer, Álvaro
Holtrop, G.
Lobley, Gerald E.
author_role author
author2 Balcells Terés, Joaquim
Fondevila, Manuel
Belenguer, Álvaro
Holtrop, G.
Lobley, Gerald E.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Caecotrophy
Rabbit does
Microbial lysine
topic Caecotrophy
Rabbit does
Microbial lysine
description The contribution of microbial amino acids through caecotrophy to tissue protein metabolism was investigated in lactating does. Attempts were made to vary microbial supply through a dietary antibiotic, Zn bacitracin, and to vary tissue demand through manipulation of litter size. Three groups of eight New Zealand does were fed different experimental diets from day 28 of pregnancy to day 26 of lactation. The control group received the basal diet formulated to meet requirements with grass hay, wheat, soyabean meal and barley grain. The second (no antibiotic) group and the third (bacitracin; BAC) group ingested the basal diet supplemented with ammonium sulfate (5 g/kg), initially unlabelled (day 1 to day 8) then labelled with 15N (day 9 to day 30), while the BAC diet was also supplemented throughout with antibiotic (Zn bacitracin; 100 mg/kg). From just after birth each group of does was subdivided into two groups, each of four females, with the litter size either five (LS5) or nine (LS9) pups. The 15N enrichment in liver, milk and caecal bacteria amino acids was determined by GC-combustion-isotope ratio MS. All amino acids in bacterial protein were enriched with the (15NH4)2SO4 treatment, with lysine 15N enrichment significantly greater in caecal bacteria (0·23 (SE 0·0063) atom % excess (ape)) than in liver (0·04 (SE 0·0004) ape) or milk protein (0·05 (SE 0·0018) ape), confirming the double origin (bacterial and dietary) of tissue lysine. The contribution of microbes to tissue lysine was 0·23 (SE 0·006) when milk protein was used as reference.
publishDate 2008
dc.date.none.fl_str_mv 2008
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1017/S0007114508957986
http://hdl.handle.net/10459.1/64879
url https://doi.org/10.1017/S0007114508957986
http://hdl.handle.net/10459.1/64879
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.1017/S0007114508957986
British Journal of Nutrition, 2008, vol. 100, núm. 5, p. 977-983
dc.rights.none.fl_str_mv (c) Cambridge University Press, 2008
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Cambridge University Press, 2008
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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