Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium
The retinal pigment epithelium (RPE) is progressively degenerated during age-related macular degeneration (AMD), one of the leading causes of irreversible blindness, which clinical hallmark is the buildup of sub-RPE extracellular material. Clinical observations indicate that Zn dyshomeostasis can in...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/339030 |
| Acceso en línea: | http://hdl.handle.net/10261/339030 |
| Access Level: | acceso abierto |
| Palabra clave: | Retinal pigment epithelium Cell culture Sub-RPE deposits AMD in vitro model Zinc dyshomeostasis Physical barrier |
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Altered zinc homeostasis in a primary cell culture model of the retinal pigment epitheliumÁlvarez-Barrios, AnaÁlvarez, LydiaArtime, EnolGarcía, MontserratLengyel, ImrePereiro, RosarioGonzález-Iglesias, HéctorRetinal pigment epitheliumCell cultureSub-RPE depositsAMD in vitro modelZinc dyshomeostasisPhysical barrierThe retinal pigment epithelium (RPE) is progressively degenerated during age-related macular degeneration (AMD), one of the leading causes of irreversible blindness, which clinical hallmark is the buildup of sub-RPE extracellular material. Clinical observations indicate that Zn dyshomeostasis can initiate detrimental intracellular events in the RPE. In this study, we used a primary human fetal RPE cell culture model producing sub-RPE deposits accumulation that recapitulates features of early AMD to study Zn homeostasis and metalloproteins changes. RPE cell derived samples were collected at 10, 21 and 59 days in culture and processed for RNA sequencing, elemental mass spectrometry and the abundance and cellular localization of specific proteins. RPE cells developed processes normal to RPE, including intercellular unions formation and expression of RPE proteins. Punctate deposition of apolipoprotein E, marker of sub-RPE material accumulation, was observed from 3 weeks with profusion after 2 months in culture. Zn cytoplasmic concentrations significantly decreased 0.2 times at 59 days, from 0.264 ± 0.119 ng·μg at 10 days to 0.062 ± 0.043 ng·μg at 59 days (p < 0.05). Conversely, increased levels of Cu (1.5-fold in cytoplasm, 5.0-fold in cell nuclei and membranes), Na (3.5-fold in cytoplasm, 14.0-fold in cell nuclei and membranes) and K (6.8-fold in cytoplasm) were detected after 59-days long culture. The Zn-regulating proteins metallothioneins showed significant changes in gene expression over time, with a potent down-regulation at RNA and protein level of the most abundant isoform in primary RPE cells, from 0.141 ± 0.016 ng·mL at 10 days to 0.056 ± 0.023 ng·mL at 59 days (0.4-fold change, p < 0.05). Zn influx and efflux transporters were also deregulated, along with an increase in oxidative stress and alterations in the expression of antioxidant enzymes, including superoxide dismutase, catalase and glutathione peroxidase. The RPE cell model producing early accumulation of extracellular deposits provided evidences on an altered Zn homeostasis, exacerbated by changes in cytosolic Zn-binding proteins and Zn transporters, along with variations in other metals and metalloproteins, suggesting a potential role of altered Zn homeostasis during AMD development.The Instituto Oftalmológico Fernández-Vega and Fundación de Investigación Oftalmológica acknowledge financial support from the Fundación Rafael del Pino (http://www.frdelpino.es), through the “Cátedra Rafael del Pino.” This work was partially financed through project (PID2019-107838RB-I00/Agencia Estatal de Investigación (AEI)/10.13039/501100011033). AÁ-B acknowledges the FPU grant (Ref. FPU20/00608, Ministry of Universities of Spain).Frontiers MediaFundación de Investigación Oftalmológica Fernández-VegaFundación Rafael del PinoAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/339030reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107838RB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at http://dx.doi.org/10.3389/fnut.2023.1124987http://dx.doi.org/10.3389/fnut.2023.1124987Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3390302026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium |
| title |
Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium |
| spellingShingle |
Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium Álvarez-Barrios, Ana Retinal pigment epithelium Cell culture Sub-RPE deposits AMD in vitro model Zinc dyshomeostasis Physical barrier |
| title_short |
Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium |
| title_full |
Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium |
| title_fullStr |
Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium |
| title_full_unstemmed |
Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium |
| title_sort |
Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium |
| dc.creator.none.fl_str_mv |
Álvarez-Barrios, Ana Álvarez, Lydia Artime, Enol García, Montserrat Lengyel, Imre Pereiro, Rosario González-Iglesias, Héctor |
| author |
Álvarez-Barrios, Ana |
| author_facet |
Álvarez-Barrios, Ana Álvarez, Lydia Artime, Enol García, Montserrat Lengyel, Imre Pereiro, Rosario González-Iglesias, Héctor |
| author_role |
author |
| author2 |
Álvarez, Lydia Artime, Enol García, Montserrat Lengyel, Imre Pereiro, Rosario González-Iglesias, Héctor |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Fundación de Investigación Oftalmológica Fernández-Vega Fundación Rafael del Pino Agencia Estatal de Investigación (España) Ministerio de Ciencia, Innovación y Universidades (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Retinal pigment epithelium Cell culture Sub-RPE deposits AMD in vitro model Zinc dyshomeostasis Physical barrier |
| topic |
Retinal pigment epithelium Cell culture Sub-RPE deposits AMD in vitro model Zinc dyshomeostasis Physical barrier |
| description |
The retinal pigment epithelium (RPE) is progressively degenerated during age-related macular degeneration (AMD), one of the leading causes of irreversible blindness, which clinical hallmark is the buildup of sub-RPE extracellular material. Clinical observations indicate that Zn dyshomeostasis can initiate detrimental intracellular events in the RPE. In this study, we used a primary human fetal RPE cell culture model producing sub-RPE deposits accumulation that recapitulates features of early AMD to study Zn homeostasis and metalloproteins changes. RPE cell derived samples were collected at 10, 21 and 59 days in culture and processed for RNA sequencing, elemental mass spectrometry and the abundance and cellular localization of specific proteins. RPE cells developed processes normal to RPE, including intercellular unions formation and expression of RPE proteins. Punctate deposition of apolipoprotein E, marker of sub-RPE material accumulation, was observed from 3 weeks with profusion after 2 months in culture. Zn cytoplasmic concentrations significantly decreased 0.2 times at 59 days, from 0.264 ± 0.119 ng·μg at 10 days to 0.062 ± 0.043 ng·μg at 59 days (p < 0.05). Conversely, increased levels of Cu (1.5-fold in cytoplasm, 5.0-fold in cell nuclei and membranes), Na (3.5-fold in cytoplasm, 14.0-fold in cell nuclei and membranes) and K (6.8-fold in cytoplasm) were detected after 59-days long culture. The Zn-regulating proteins metallothioneins showed significant changes in gene expression over time, with a potent down-regulation at RNA and protein level of the most abundant isoform in primary RPE cells, from 0.141 ± 0.016 ng·mL at 10 days to 0.056 ± 0.023 ng·mL at 59 days (0.4-fold change, p < 0.05). Zn influx and efflux transporters were also deregulated, along with an increase in oxidative stress and alterations in the expression of antioxidant enzymes, including superoxide dismutase, catalase and glutathione peroxidase. The RPE cell model producing early accumulation of extracellular deposits provided evidences on an altered Zn homeostasis, exacerbated by changes in cytosolic Zn-binding proteins and Zn transporters, along with variations in other metals and metalloproteins, suggesting a potential role of altered Zn homeostasis during AMD development. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10261/339030 |
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http://hdl.handle.net/10261/339030 |
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Inglés |
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Inglés |
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#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107838RB-I00 The underlying dataset has been published as supplementary material of the article in the publisher platform at http://dx.doi.org/10.3389/fnut.2023.1124987 http://dx.doi.org/10.3389/fnut.2023.1124987 Sí |
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Frontiers Media |
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