Corneal sensory nerve activity in an experimental model of UV keratitis

[Purpose] To produce in guinea pigs a UV-induced keratitis, to analyze the effects of this pathology on corneal nerve activity.

Detalles Bibliográficos
Autores: Acosta, M. Carmen, Luna, Carolina, Quirce, Susana, Belmonte, Carlos, Gallar, Juana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/289549
Acceso en línea:http://hdl.handle.net/10261/289549
Access Level:acceso abierto
Palabra clave:UV-induced inflammation
Photokeratitis
Corneal innervation
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spelling Corneal sensory nerve activity in an experimental model of UV keratitisAcosta, M. CarmenLuna, CarolinaQuirce, SusanaBelmonte, CarlosGallar, JuanaUV-induced inflammationPhotokeratitisCorneal innervation[Purpose] To produce in guinea pigs a UV-induced keratitis, to analyze the effects of this pathology on corneal nerve activity.[Methods] In anesthetized animals, one eye was exposed to 254 nm UV-C radiation (500-1000 mJ/cm(2)), excised 24 to 48 hours later and superfused in vitro. Nerve impulse activity was recorded in ciliary nerve filaments or in corneal sensory terminals of intact and UV-irradiated eyes. Impulse activity in response to mechanical (von Frey hairs), chemical (98.5% CO2 gas jets), and thermal stimulation (cooling from 34°C to 20°C; heating to 50°C) was analyzed. Duration of eyelid closure and blinking and tearing rates were evaluated in control and in UV-irradiated eyes, before and after application of TRPV1, TRPA1, and TRPM8 agonists (100 μM capsaicin; 10 mM AITC, and 200 μM menthol, respectively).[Results] After irradiation, mechanical threshold of mechano-nociceptor corneo-scleral fibers was reduced (0.59 ± 0.4 vs. 0.27 ± 0.07 mN; P < 0.05) while polymodal nociceptors increased their response to chemical stimulation (1.7 ± 0.2 vs. 3.4 ± 0.5 imps/s; P < 0.05). In contrast, cold thermoreceptors showed a significantly lower ongoing activity at 34°C (8.6 ± 0.5 vs. 6.1 ± 0.9 imp/s; P < 0.05) and a reduced responsiveness to cooling pulses (peak frequency = 29.8 ± 1.3 vs. 18.9 ± 1.8 imp/s; P < 0.001). Blinking but not tearing rate was significantly higher; behavioral responses to topical capsaicin and AITC, but not to menthol were enhanced in UV-irradiated animals.[Conclusions] Sensitization of nociceptor and depression of cold thermoreceptor activity following UV radiation appear to result from an action of inflammatory mediators on TRP channels selectively expressed by sensory nerve terminals. Changes in nerve activity possibly underlie discomfort sensations associated with corneo-conjunctival inflammation induced by UV exposure.Supported in part by Grants GV/2007/030 (MCA) from Generalitat Valenciana, Spain, and SAF2011-22500 (JG), and by Grant BFU2008-04425 (CB) from the Ministerio de Economía y Competitividad, Spain.Peer reviewedAssociation for Research in Vision and OphthalmologyMinisterio de Economía y Competitividad (España)Generalitat ValencianaMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232014info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/289549reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//SAF2011-22500info:eu-repo/grantAgreement/MICINN//BFU2008-04425Investigative Ophthalmology and Visual Sciencehttps://doi.org/10.1167/iovs.13-13774Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2895492026-05-22T06:33:51Z
dc.title.none.fl_str_mv Corneal sensory nerve activity in an experimental model of UV keratitis
title Corneal sensory nerve activity in an experimental model of UV keratitis
spellingShingle Corneal sensory nerve activity in an experimental model of UV keratitis
Acosta, M. Carmen
UV-induced inflammation
Photokeratitis
Corneal innervation
title_short Corneal sensory nerve activity in an experimental model of UV keratitis
title_full Corneal sensory nerve activity in an experimental model of UV keratitis
title_fullStr Corneal sensory nerve activity in an experimental model of UV keratitis
title_full_unstemmed Corneal sensory nerve activity in an experimental model of UV keratitis
title_sort Corneal sensory nerve activity in an experimental model of UV keratitis
dc.creator.none.fl_str_mv Acosta, M. Carmen
Luna, Carolina
Quirce, Susana
Belmonte, Carlos
Gallar, Juana
author Acosta, M. Carmen
author_facet Acosta, M. Carmen
Luna, Carolina
Quirce, Susana
Belmonte, Carlos
Gallar, Juana
author_role author
author2 Luna, Carolina
Quirce, Susana
Belmonte, Carlos
Gallar, Juana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Generalitat Valenciana
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv UV-induced inflammation
Photokeratitis
Corneal innervation
topic UV-induced inflammation
Photokeratitis
Corneal innervation
description [Purpose] To produce in guinea pigs a UV-induced keratitis, to analyze the effects of this pathology on corneal nerve activity.
publishDate 2014
dc.date.none.fl_str_mv 2014
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/289549
url http://hdl.handle.net/10261/289549
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//SAF2011-22500
info:eu-repo/grantAgreement/MICINN//BFU2008-04425
Investigative Ophthalmology and Visual Science
https://doi.org/10.1167/iovs.13-13774
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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