Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization

9 pages, 4 figures, 4 tables

Detalles Bibliográficos
Autores: Valcárcel Barros, Jesús, García, Miriam R., Sampayo, Lucía F., Vázquez, José Antonio
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/193588
Acceso en línea:http://hdl.handle.net/10261/193588
Access Level:acceso abierto
Palabra clave:Chondroitin sulfate
Glycosaminoglycan
Hyaluronidase
Chondroitinase
Enzymatic depolymerization
Depolymerization kinetics
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spelling Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerizationValcárcel Barros, JesúsGarcía, Miriam R.Sampayo, Lucía F.Vázquez, José AntonioChondroitin sulfateGlycosaminoglycanHyaluronidaseChondroitinaseEnzymatic depolymerizationDepolymerization kinetics9 pages, 4 figures, 4 tablesChondroitin sulfate (CS) is a sulfated glycosaminoglycan with diverse biological activities, which are influenced by molecular weight (Mw) and sulfation pattern. In the present work, we take advantage of the characteristic high Mw of fish CS (51–70 kDa) to obtain lower Mw fragments with hyaluronidase and chondroitinase ABC. With this aim, we present a pseudo-mechanistic model capable of reproducing the decrease in Mw of CS from five different fish species over 24 h at four enzyme to substrate ratios. The fitting parameters of the model for each species allow to establish conditions of reaction to produce CS of the desired Mw. Furthermore, the main features of the sulfation pattern of fish CS remain in the depolymerized fragments, highlighting the feasibility of the proposed approachThis study was financed by projects IBEROS (0245_IBEROS_1_E, POCTEP 2015), CVMar+I (0302_CVMAR_I_1_P, POCTEP 2015), BLUEHUMAN (EAPA_151/2016, UE-INTERREG Atlantic Area Programme) and Xunta de Galicia (Grupos de Potencial Crecimiento, IN607B 2018/19). M.R.G thanks RTI2018-093560-J-I00 (MCIU/AEI/FEDER, UE)Peer reviewedElsevierInterregXunta de GaliciaMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/193588reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093560-J-I00The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.1016/j.carbpol.2019.115450https://doi.org/10.1016/j.carbpol.2019.115450Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1935882026-05-22T06:33:51Z
dc.title.none.fl_str_mv Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization
title Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization
spellingShingle Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization
Valcárcel Barros, Jesús
Chondroitin sulfate
Glycosaminoglycan
Hyaluronidase
Chondroitinase
Enzymatic depolymerization
Depolymerization kinetics
title_short Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization
title_full Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization
title_fullStr Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization
title_full_unstemmed Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization
title_sort Marine chondroitin sulfate of defined molecular weight by enzymatic depolymerization
dc.creator.none.fl_str_mv Valcárcel Barros, Jesús
García, Miriam R.
Sampayo, Lucía F.
Vázquez, José Antonio
author Valcárcel Barros, Jesús
author_facet Valcárcel Barros, Jesús
García, Miriam R.
Sampayo, Lucía F.
Vázquez, José Antonio
author_role author
author2 García, Miriam R.
Sampayo, Lucía F.
Vázquez, José Antonio
author2_role author
author
author
dc.contributor.none.fl_str_mv Interreg
Xunta de Galicia
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chondroitin sulfate
Glycosaminoglycan
Hyaluronidase
Chondroitinase
Enzymatic depolymerization
Depolymerization kinetics
topic Chondroitin sulfate
Glycosaminoglycan
Hyaluronidase
Chondroitinase
Enzymatic depolymerization
Depolymerization kinetics
description 9 pages, 4 figures, 4 tables
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/193588
url http://hdl.handle.net/10261/193588
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093560-J-I00
The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.1016/j.carbpol.2019.115450
https://doi.org/10.1016/j.carbpol.2019.115450
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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