Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)

11 páginas, 5 figguras, 2 tablas

Bibliographic Details
Authors: Vázquez-Sánchez, Daniel, López Cabo, Marta, Saá Ibusquiza, Paula, Rodríguez Herrera, Juan José
Format: article
Publication Date:2012
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/52501
Online Access:http://hdl.handle.net/10261/52501
Access Level:Open access
Keyword:Staphylococcus aureus
Fishery products
Retail level
Enterotoxin genes
Antibiotic resistance
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spelling Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)Vázquez-Sánchez, DanielLópez Cabo, MartaSaá Ibusquiza, PaulaRodríguez Herrera, Juan JoséStaphylococcus aureusFishery productsRetail levelEnterotoxin genesAntibiotic resistance11 páginas, 5 figguras, 2 tablasA total of 298 fishery products purchased from retail outlets in Galicia (NW Spain) between January 2008 and May 2009 were analyzed for the presence of Staphylococcus aureus. S. aureus was detected in a significant proportion of products (~ 25%). Incidence was highest in fresh (43%) and frozen products (30%), but it was high in all other categories: salted fish (27%), smoked fish (26%), ready-to-cook products (25%), non-frozen surimis (20%), fish roes (17%) and other ready-to-eat products (10%). A significant proportion of smoked fish, surimis, fish roes and other ready-to-eat products did not comply with legal limits in force. RAPD-PCR of 125 S. aureus isolated from fishery products was carried out using three primers (AP-7, ERIC-2 and S). Isolates displayed 33 fingerprint patterns. Each pattern was attributed to a single bacterial clone. Cluster analysis based on similarity values between RAPD fingerprints did not find relationship between any RAPD pattern and any product category. Isolates were also tested for se genes and susceptibility to a range of antibiotics (cephalothin, clindamycin, chloramphenicol, erythromycin, gentamicin, oxacillin, penicillin G, tetracycline, vancomycin, methicillin, ciprofloxacin and trimethoprim-sulfamethoxazole). Most isolates (88%) were found to be sea positive. Putative enterotoxigenic strains counts reached high risk levels in 17 products. No relationship was found between the presence of se genes and RAPD patterns. All isolates were resistant to penicillin, chloramphenicol and ciprofloxacin, and most to tetracycline (82.4%), but none was methicillin-resistant. A revision of pre-requisite programs leading to improve hygienic practices in handling and processing operations from fishing or farming to retail is recommended to ensure fishery products safety.This work was financially supported by the Xunta de Galicia (PGIDIT 07 TAL 014402PR). Authors DVS and PSI were supported by a research grant (JAE Program) by CSIC. Author JJRH was supported by the Ramón y Cajal Programme (co-financed by the European Social Fund).Peer reviewedElsevier201220122012info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/52501reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.ijfoodmicro.2012.05.021info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/525012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)
title Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)
spellingShingle Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)
Vázquez-Sánchez, Daniel
Staphylococcus aureus
Fishery products
Retail level
Enterotoxin genes
Antibiotic resistance
title_short Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)
title_full Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)
title_fullStr Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)
title_full_unstemmed Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)
title_sort Incidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (Northwest Spain)
dc.creator.none.fl_str_mv Vázquez-Sánchez, Daniel
López Cabo, Marta
Saá Ibusquiza, Paula
Rodríguez Herrera, Juan José
author Vázquez-Sánchez, Daniel
author_facet Vázquez-Sánchez, Daniel
López Cabo, Marta
Saá Ibusquiza, Paula
Rodríguez Herrera, Juan José
author_role author
author2 López Cabo, Marta
Saá Ibusquiza, Paula
Rodríguez Herrera, Juan José
author2_role author
author
author
dc.subject.none.fl_str_mv Staphylococcus aureus
Fishery products
Retail level
Enterotoxin genes
Antibiotic resistance
topic Staphylococcus aureus
Fishery products
Retail level
Enterotoxin genes
Antibiotic resistance
description 11 páginas, 5 figguras, 2 tablas
publishDate 2012
dc.date.none.fl_str_mv 2012
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/52501
url http://hdl.handle.net/10261/52501
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.ijfoodmicro.2012.05.021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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